Estadísticas

 array:18 [
  "pii" => "13111840"
  "issn" => "0213005X"
  "estado" => "S300"
  "fechaPublicacion" => "2007-10-30"
  "documento" => "article"
  "crossmark" => 0
  "subdocumento" => "fla"
  "cita" => "Enferm Infecc Microbiol Clin. 2007;25 Supl 3:66-71"
  "abierto" => array:3 [
    "ES" => false
    "ES2" => false
    "LATM" => false
  ]
  "gratuito" => false
  "lecturas" => array:2 [
    "total" => 4445
    "formatos" => array:3 [
      "EPUB" => 9
      "HTML" => 1804
      "PDF" => 2632
    ]
  ]
  "itemAnterior" => array:15 [
    "pii" => "13111839"
    "issn" => "0213005X"
    "estado" => "S300"
    "fechaPublicacion" => "2007-10-30"
    "documento" => "article"
    "crossmark" => 0
    "subdocumento" => "fla"
    "cita" => "Enferm Infecc Microbiol Clin. 2007;25 Supl 3:60-5"
    "abierto" => array:3 [
      "ES" => false
      "ES2" => false
      "LATM" => false
    ]
    "gratuito" => false
    "lecturas" => array:2 [
      "total" => 2158
      "formatos" => array:3 [
        "EPUB" => 10
        "HTML" => 1598
        "PDF" => 550
      ]
    ]
    "es" => array:10 [
      "idiomaDefecto" => true
      "titulo" => "Virus respiratorios: viejos y nuevos virus. Revisión de métodos diagnósticos"
      "tienePdf" => "es"
      "tieneTextoCompleto" => 0
      "tieneResumen" => array:2 [
        0 => "es"
        1 => "en"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "60"
          "paginaFinal" => "65"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "en" => array:1 [
          "titulo" => "Respiratory viruses: old and new. Review of diagnostic methods"
        ]
      ]
      "contieneResumen" => array:2 [
        "es" => true
        "en" => true
      ]
      "contienePdf" => array:1 [
        "es" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "José María Navarro-Marí, Mercedes Pérez-Ruiz"
          "autores" => array:2 [
            0 => array:2 [
              "nombre" => "José María"
              "apellidos" => "Navarro-Marí"
            ]
            1 => array:2 [
              "nombre" => "Mercedes"
              "apellidos" => "Pérez-Ruiz"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "es"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/13111839?idApp=UINPBA00004N"
    "url" => "/0213005X/00000025000000S3/v0_201307121326/13111839/v0_201307121326/es/main.assets"
  ]
  "es" => array:11 [
    "idiomaDefecto" => true
    "titulo" => "Métodos moleculares para la determinación del genotipo del virus de la hepatitis C"
    "tieneTextoCompleto" => 0
    "paginas" => array:1 [
      0 => array:2 [
        "paginaInicial" => "66"
        "paginaFinal" => "71"
      ]
    ]
    "autores" => array:1 [
      0 => array:3 [
        "autoresLista" => "David Navarro Ortega, Marta Jiménez Mayordomo, M Desamparados Martínez Aparicio"
        "autores" => array:3 [
          0 => array:3 [
            "nombre" => "David"
            "apellidos" => "Navarro Ortega"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "affa"
              ]
            ]
          ]
          1 => array:3 [
            "nombre" => "Marta"
            "apellidos" => "Jim&#233;nez Mayordomo"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "affb"
              ]
            ]
          ]
          2 => array:3 [
            "nombre" => "M Desamparados"
            "apellidos" => "Mart&#237;nez Aparicio"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "affb"
              ]
            ]
          ]
        ]
        "afiliaciones" => array:2 [
          0 => array:3 [
            "entidad" => "Servicio de Microbiología. Hospital Clínico Universitario de Valencia. Valencia. España. Departamento de Microbiología. Facultad de Medicina de Valencia. Valencia. España."
            "etiqueta" => "<span class="elsevierStyleSup">a</span>"
            "identificador" => "affa"
          ]
          1 => array:3 [
            "entidad" => "Servicio de Microbiología. Hospital Clínico Universitario de Valencia. Valencia. España."
            "etiqueta" => "<span class="elsevierStyleSup">b</span>"
            "identificador" => "affb"
          ]
        ]
      ]
    ]
    "titulosAlternativos" => array:1 [
      "en" => array:1 [
        "titulo" => "Molecular methods for genotypic determination of the hepatitis C virus"
      ]
    ]
    "pdfFichero" => "28v25nSupl.3a13111840pdf001.pdf"
    "tienePdf" => true
    "PalabrasClave" => array:2 [
      "es" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Palabras clave"
          "identificador" => "xpalclavsec205230"
          "palabras" => array:5 [
            0 => "Virus de la hepatitis C"
            1 => "Genotipo"
            2 => "Subtipo"
            3 => "Secuenciaci&#243;n directa"
            4 => "LiPA"
          ]
        ]
      ]
      "en" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Keywords"
          "identificador" => "xpalclavsec205231"
          "palabras" => array:5 [
            0 => "Hepatitis C virus &#40;HCV&#41;"
            1 => "Genotype"
            2 => "Subtype"
            3 => "Direct sequencing"
            4 => "LiPA"
          ]
        ]
      ]
    ]
    "tieneResumen" => true
    "resumen" => array:2 [
      "es" => array:1 [
        "resumen" => "Se han descrito 6 genotipos y hasta 80 subtipos del virus de la hepatitis C &#40;VHC&#41;&#46; El conocimiento del genotipo infectante es crucial para tratar adecuadamente al paciente&#46; El m&#233;todo de referencia para la tipificaci&#243;n molecular del VHC es la secuenciaci&#243;n completa del genoma viral y su posterior an&#225;lisis filogen&#233;tico&#46; Sin embargo&#44; esta posibilidad no est&#225; al alcance de la mayor&#237;a de los laboratorios hospitalarios&#46; Existen varios m&#233;todos alternativos comercializados&#59; los de uso m&#225;s extendido se basan en el an&#225;lisis de la regi&#243;n 5&#191;UTR mediante amplificaci&#243;n y posterior secuenciaci&#243;n directa &#40;TRUGENE 5&#191;NC Genotyping Kit&#59; Visible Genetics&#44; Toronto&#44; Ontario&#44; Canad&#225;&#41; o hibridaci&#243;n inversa de los amplicones con sondas dispuestas en l&#237;nea sobre membrana-LiPA- &#40;INNO-LiPA HCV I&#47;II&#47; VERSANT HCV Genotype Assay 1&#46;0&#59; Innogenetics&#44; N&#46;V&#46;&#44; Zwijnaarde&#44; Bayer Healthcare&#44; B&#233;lgica&#41;&#46; Los resultados obtenidos con estos ensayos son generalmente superponibles&#59; identifican correctamente los genotipos en un 95-100&#37; &#40;clasifican algunos genotipos 4 y 6 como genotipos 1&#41;&#44; pero su &#237;ndice de acierto es menor &#40;70-85&#37;&#41; con los subtipos&#44; particularmente 1a&#44;1b&#44; 2a&#47;2c y 4a&#47;4c&#46; Existe una nueva generaci&#243;n de pruebas comercializadas con mejores prestaciones&#58; GEN-ETI-K DEIA kit &#40;Sorin&#44; Saluggia&#44; Italy&#41; que analiza la regi&#243;n core&#59; TRUGENE NS5B Genotyping Assay &#40;Bayer Healhcare&#44; B&#233;lgica&#41;&#44; la regi&#243;n NS5B&#59; Real Time HCV Genotyping Test &#40;Abbott&#44; MS&#41; las regiones 5&#191;UTR y la NS5B y VERSANT 2&#46;0 &#40;Bayer&#41;&#44; las regiones 5&#191;UTR y core&#59; estos ensayos son muy certeros en la determinaci&#243;n del genotipo y de la mayor&#237;a de subtipos conflictivos&#44; particularmente 1a y 1b&#46;"
      ]
      "en" => array:1 [
        "resumen" => "Six genotypes and up to 80 subtypes of hepatitis C virus &#40;HCV&#41; have been described&#46; Knowledge of the infecting genotype is crucial for appropriate therapeutic management of HCV infection&#46; Whole genome sequencing and subsequent phylogenetic analysis is the gold standard for HCV molecular typing&#59; however&#44; this procedure cannot be implemented in routine clinical laboratories&#46; A number of alternative methods have been commercialized&#44; the most widely used being those that target the 5&#191;UTR region&#46; In these assays&#44; amplicons are first generated and then analyzed by direct sequencing &#40;TRUGENE 5&#191;NC genotyping kit&#59; Visible Genetics&#44; Toronto&#44; Notario&#44; Canada&#41; or by reverse hybridization of amplicons with membrane immobilized line probes -LiPA- &#40;INNO-LiPA HCV I&#47;II&#47; VERSANT HCV genotype assay 1&#46;0&#59; Innogenetics&#44; N&#46;V&#46;&#44; Zwijnaarde&#44; Belgium&#47;Bayer Healthcare&#41;&#46; Both assays produce comparable results&#58; the overall accuracy for genotype determination is 95-100&#37; &#40;a number of genotypes 4 and 6 are misclassified as genotype 1&#41;&#44; but only 70-85&#37; for subtyping &#40;with frequent mistyping of subtypes 1a&#44; 1b&#44; 2a&#47;2c&#44; 4a&#47;4c&#41;&#46; A new generation of commercialized assays with better performance is currently available&#58; GEN-ETI-K DEIA kit &#40;Sorin&#44; Saluggia&#44; Italy&#41;&#44; which analyzes the core region&#44; TRUGENE NS5B genotyping assay &#40;Bayer Healhcare&#41; for the NS5B region&#44; RealTime HCV Genotyping Test &#40;Abbott&#44; MS&#41; for both 5&#191;UTR and NS5B regions and VERSANT 2&#46;0 &#40;Bayer&#41;&#44; both for the 5&#191;UTR and core regions&#59; these assays are highly accurate for genotype determination and typing of most difficult subtypes&#44; particularly 1a and 1b&#46;"
      ]
    ]
  ]
  "idiomaDefecto" => "es"
  "url" => "/0213005X/00000025000000S3/v0_201307121326/13111840/v0_201307121326/es/main.assets"
  "Apartado" => array:4 [
    "identificador" => "15268"
    "tipo" => "SECCION"
    "es" => array:2 [
      "titulo" => "Programa de Control Externo de Calidad SEIMC&#44; a&#241;o 2006"
      "idiomaDefecto" => true
    ]
    "idiomaDefecto" => "es"
  ]
  "PDF" => "https://static.elsevier.es/multimedia/0213005X/00000025000000S3/v0_201307121326/13111840/v0_201307121326/es/28v25nSupl.3a13111840pdf001.pdf?idApp=UINPBA00004N&text.app=https://www.elsevier.es/"
  "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/13111840?idApp=UINPBA00004N"
]