Estadísticas

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      "titulo" => "Enfermedad por Mycobacterium kansasii"
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          "titulo" => "Disease by Mycobacterium kansasii"
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          "autoresLista" => "J Repáraz"
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      "titulo" => "Adherencia al tratamiento antirretroviral de gran actividad: impacto de una intervención de asesoramiento individualizado"
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          "titulo" => "Adherence to very active antiretroviral treatment: impact of individualized assessment"
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          "autoresLista" => "H Knobel, A Carmona, JL López, JL Gimeno, P Saballs, A González, A Guelar, A Díez"
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    "titulo" => "Comparación entre centrifugación y sedimentación previa al cultivo en el aislamiento de citomegalovirus en la orina de receptores de trasplante renal"
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        "titulo" => "Comparison between centrifugation and sedimentation prior to cultures in the isolation of cytomegalovirus in the urine of renal transplant receptors"
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        "resumen" => "<span class="elsevierStyleBold">Fundamento&#58;</span> Realizar un estudio comparativo entre el proceso de centrifugaci&#243;n convencional y la sedimentaci&#243;n de la orina en su capacidad para detectar la presencia de citomegalovirus en esta muestra&#46; <br/> <span class="elsevierStyleBold">Material y m&#233;todos&#58;</span> Se han estudiado las orinas procedentes de 45 pacientes receptores de un trasplante renal&#46; Cada una de las muestras se dividi&#243; en dos partes y&#44; tras su descontaminaci&#243;n&#44; una de ellas fue sometida a centrifugaci&#243;n &#40;1&#46;500 rpm durante 10 min&#41; y la otra a sedimentaci&#243;n a temperatura ambiente durante 30 min&#46; Del sobrenadante de cada una de ellas se sembraron 250 &#181;l en un shell-vial de la l&#237;nea MRC-5&#46; Los cultivos se incubaron durante 48 h a 37 oC y fueron revelados mediante una inmunofluorescencia indirecta&#46; Las orinas consideradas como t&#243;xicas totales fueron resembradas despu&#233;s de su diluci&#243;n al 1&#58;1 con medio de mantenimiento&#46; <br/> <span class="elsevierStyleBold">Resultados&#58;</span> Se han analizado 845 orinas&#44; 743 &#40;88&#37;&#41; fueron negativas y 102 &#40;12&#37;&#41; positivas&#59; 66 &#40;7&#44;8&#37;&#41; fueron consideradas como t&#243;xicas&#58; 35 &#40;4&#44;1&#37;&#41; con toxicidad parcial y 31 &#40;3&#44;6&#37;&#41; con toxicidad total&#46; Las orinas centrifugadas fueron positivas en el 86&#44;2&#37; de los casos frente al 98&#37; de las sedimentadas &#40;p &#61; 0&#44;004&#41;&#46; De las 31 orinas consideradas como t&#243;xicas totales&#44; 17 &#40;2&#44;2&#37;&#41; correspond&#237;an a orinas negativas y 14 &#40;13&#44;7&#37;&#41; a las positivas&#46; De ellas&#44; 12 &#40;85&#44;7&#37;&#41; correspond&#237;an a las orinas centrifugadas y 2 &#40;14&#44;3&#37;&#41; a las sedimentadas &#40;p &#61; 0&#44;001&#41;&#46; <br/> <span class="elsevierStyleBold">Conclusiones&#58;</span> El porcentaje de toxicidad de las orinas ha sido bajo &#40;7&#44;8&#37;&#41;&#44; lo cual parece no recomendar la diluci&#243;n sistem&#225;tica de todas las orinas&#46; El proceso de sedimentaci&#243;n ha permitido detectar un mayor n&#250;mero de orinas positivas para citomegalovirus con una menor toxicidad sobre las monocapas&#46; La utilizaci&#243;n de este proceso disminuir&#237;a el n&#250;mero de orinas que precisar&#237;an ser resembradas"
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        "resumen" => "<span class="elsevierStyleBold">Background&#58;</span> To compare the capacity of conventional centrifugation and spontaneous sedimentation to detect the presence of cytomegalovirus &#40;CMV&#41; in urine samples&#46; <br/> <span class="elsevierStyleBold">Material and Methods&#58;</span> We studied urine samples from 45 renal transplant recipients&#46; After decontamination half of each sample was centrifuged &#40;1&#44;500 rpm for 10 minutes&#41; and the other half was allowed to sediment at room temperature for 30 minutes&#46; From the supernatant of each of these 250 &#181;l was inoculated in a shell-vial &#40;MRC-5&#41;&#46; Cultures were incubated for 48 hours at 37 &#176;C and stained by an indirect immunofluorescence assay&#46; The samples considered totally toxic were re-inoculated after dilution 1&#58;1 with maintainance medium&#46; <br/> <span class="elsevierStyleBold">Results&#58;</span> Of 845 urine samples analyzed&#44; 743 &#40;88&#37;&#41; were negative and 102 &#40;12&#37;&#41; positive&#46; 66 &#40;7&#46;8&#37;&#41; were considered toxic&#58; 35 &#40;4&#46;1&#37;&#41; partially toxic and 31 &#40;3&#46;6&#37;&#41; totally toxic&#46; Of the centrifuged urine samples 86&#46;2&#37; were positive against 98&#37; of the sedimented samples &#40;p &#61; 0&#46;004&#41;&#46; Of the 31 samples considered totally toxic&#44; 17 &#40;2&#46;2&#37;&#41; corresponded with negative urine samples and 14 &#40;13&#46;7&#37;&#41; with positive samples&#46; Of these&#44; 12 &#40;85&#46;7&#37;&#41; were detected in centrifuged samples and 2 &#40;14&#46;3&#37;&#41; in sedimented samples &#40;p &#61; 0&#46;001&#41;&#46; <br/> <span class="elsevierStyleBold">Conclusions&#58;</span> The percentage of toxicity in the urine samples was low &#40;7&#46;8&#37;&#41; which does not seem to suggest the need for systematic dilution of all samples&#46; The sedimentation process enabled us to detect a greater number of urine samples positive for CMV with a lower rate of toxicity of the monolayers&#46; The use of this process would reduce the number of samples requiring re-inoculation"
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