metricas
covid
Buscar en
Gastroenterología y Hepatología
Toda la web
Inicio Gastroenterología y Hepatología Accuracy of the Ultra-Rapid Urease Test for diagnosis of Helicobacter pylori inf...
Información de la revista
Vol. 40. Núm. 10.
Páginas 651-657 (diciembre 2017)
Compartir
Compartir
Descargar PDF
Más opciones de artículo
Visitas
10864
Vol. 40. Núm. 10.
Páginas 651-657 (diciembre 2017)
Original Article
Acceso a texto completo
Accuracy of the Ultra-Rapid Urease Test for diagnosis of Helicobacter pylori infection
Exactitud del test Ultra-Rapido de la Ureasa para el Diagnóstico de la infección por Helicobacter pylori
Visitas
10864
Adrian Gerald McNicholla,b,
Autor para correspondencia
adrian.mcn@gmail.com

Corresponding author.
, Julio Duconsc, Jesús Barriod, Luis Bujandab,e, Montserrat Forné-Barderaf, Reyes Aparcerog, Julio Ponceb,h, Robin Riverai, José María Dedeu-Cusoj, Pilar Garcia-Iglesiasb,k, Miguel Montorol, Alicia Bejeranoa, Yolanda Ber-Nietoc, Belen Madrigald, Eva Zapatab,e, Carmen Loras-Alastrueyf, Manuel Castrog, Andrea Nevarezh, Isabel Mendezi, Felipe Bory-Rosj..., Mireia Miquel-Planasb,k, Isabel Veral, Olga P. Nyssena,b, Javier P. Gisberta,b, on behalf of the Helicobacter pylori Study Group of the Asociación Española de Gastroenterología (AEG) Ver más
a Gastroenterology Unit, Hospital Universitario de La Princesa and Instituto de Investigación Sanitaria Princesa (IIS-IP), Madrid, Spain
b Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Madrid, Spain
c Hospital Lozano Blesa, Zaragoza, Spain
d Hospital Río Hortega, Valladolid, Spain
e Hospital de Donostia, San Sebastián, Spain
f Mutua de Terrassa, Barcelona, Spain
g Hospital de Valme, Sevilla, Spain
h Hospital La Fe, Valencia, Spain
i Hospital Costa del Sol, Málaga, Spain
j Hospital del Mar, Barcelona, Spain
k Hospital de Sabadell, Barcelona, Spain
l Hospital San Jorge, Huesca, Spain
Ver más
Este artículo ha recibido
Información del artículo
Resumen
Texto completo
Bibliografía
Descargar PDF
Estadísticas
Figuras (1)
Tablas (5)
Table 1. CrossTable of Rapid Urease Tests (by commercial brand) vs. histology in the diagnosis of H. pylori infection.
Table 2. CrossTable of Ultra Rapid Urease Test (URUT) (by time of assessment) vs. histology in the diagnosis of Helicobacter pylori infection.
Table 3. CrossTable of Rapid and Ultra Rapid Urease Tests (RUT and URUT) vs. histology in the diagnosis of Helicobacter pylori infection in patients not taking proton pump inhibitor treatment.
Table 4. Rapid and Ultra Rapid Urease Test's (RUT and URUT) accuracy for the diagnosis of Helicobacter pylori infection in all subanalyses.
Mostrar másMostrar menos
Abstract
Background

Rapid Urease Test (RUT) is a simple, cheap and relatively fast method for diagnosing Helicobacter pylori infection. It is therefore the preferred method used for patients undergoing gastroscopy. Most kits require 24h to give results. The new Ultra-Rapid Urease Test (URUT) kit by Biohit® requires less than 1h.

Objective

To determine URUT's diagnostic accuracy.

Method

Prospective, blind, multi-centre study involving dyspeptic patients. One corpus biopsy and three antral biopsies were obtained during gastroscopy for standard histological analysis, RUT and URUT. The URUT result was checked after 1min, 5min, 30min and 60min and the RUT was checked over the course of 24h. Histology was used as the gold standard test.

Results

144 patients were included, 68% female, with a mean age of 49 years old; 50% were H. pylori positive. RUT and URUT diagnoses were correct in 85.9% and 90% of the cases, respectively. The mean waiting time for a positive RUT result was 6h. The sensitivity, specificity, and positive and negative predictive values for RUT were, respectively, 82%, 90%, 89% and 84%. The URUT's results were similar (85%, 94%, 94% and 87%). These figures improved when patients taking PPIs were excluded (RUT: 86%, 91%, 93% and 83%; URUT: 91%, 94%, 96% and 89%). No statistically significant differences were found when comparing RUT and URUT distributions of correct diagnoses (McNemar's Test, p=0.3) but there was a tendency towards better results with the URUT.

Conclusion

The URUT is equivalent to (or slightly better than) the traditional RUT in diagnosing H. pylori infection, and provides results in less than an hour.

Keywords:
Helicobacter pylori
Urease
Gastroscopy
Diagnosis
Resumen
Introducción

El test de la ureasa (TRU) es un método simple, barato y relativamente rápido para el diagnóstico de la infección por Helicobacter pylori (H. pylori). Por tanto, es el método de elección en pacientes sometidos a gastroscopia. La mayoría de los kits requieren 24 h para obtener un resultado. En nuevo test ultrarrápido de la ureasa (TURU) de Biohit requiere menos de una hora.

Objetivo

Determinar la exactitud diagnóstica del TURU.

Método

Estudio multicéntrico, prospectivo y ciego, en el que se incluyó a pacientes dispépticos. Se obtuvieron 3 biopsias de antro y una de corpus durante la gastroscopia para análisis histológico estándar, TRU y TURU. El resultado del TURU se comprobó a los 1, 5, 30 y 60 min, mientras que el TRU se evaluó a lo largo de 24 h. La histología se utilizó como patrón oro.

Resultados

Se incluyó a 144 pacientes, 68% mujeres, edad media 49 años, el 50% fueron positivos para H. pylori. TRU y TURU diagnosticaron correctamente el 85,9% y 90,0% de los casos, respectivamente. La duración media de espera para un resultado positivo del TRU fue 6 h. La sensibilidad, la especificidad y los valores predictivos negativo y positivo para el TRU fueron, respectivamente, del 82, el 90, el 89 y el 84%. Los resultados del TURU fueron equivalentes (el 85, el 94, el 94 y el 87%). Estos resultados mejoraron al excluir los pacientes que tomaban IBP (TRU: 86, 91, 93 y 83%; TURU: 91, 94, 96 y 89%). La comparación de distribución de diagnósticos correctos entre TRU y TURU no encontró diferencias estadísticamente significativas (test de McNemar p=0,3) pero existe una tendencia a mejores resultados con el TURU.

Conclusión

El TURU es equivalente (o algo superior) al TRU tradicional en el diagnóstico de la infección por H. pylori y obtiene los resultados en menos de una hora.

Palabras clave:
Helicobacter pylori
Ureasa
Gastroscopia
Diagnóstico
Texto completo
Introduction

The management of Helicobacter pylori (H. pylori) infection requires accurate diagnostic methods. Several diagnostic methods, both direct (histology, culture and urease tests) and indirect (faecal antigen, serology and urea breath test), have been developed.1–4 Direct or “invasive” methods require the biopsy sampling while indirect or “non-invasive” methods detect secondary characteristics of the bacteria.5

Patients undergoing gastroscopy for dyspeptic symptoms are generally tested for H. pylori infection. Rapid Urease Test (RUT) is usually the method of choice as it is simple, cheap, gives an accurate result in 24h and the result can be observed in the endoscopic units without the involvement of other services.3–6 Urease kits have a media containing urea and a pH-dependant colour indicator, in which the biopsy is placed. H. pylori urease activity alters the pH causing a change of colour of the media.7

Common RUTs require up to 24h to obtain an accurate result, what limits its benefits and utility in clinical practice as the treatment prescription cannot be given in the moment, forcing the patient to come back for the diagnosis to the hospital's outpatient clinic.1,8 A quicker urease kit would increase efficiency by reducing costs and incommodities to patients.

Recently, new kits have been developed trying to improve the utility and speed of the urease method.5–7,9 Biohit® commercializes a new urease method that, according to its technical information, is able to give accurate results in 30min. Previous studies have evaluated the accuracy of different commercial kits obtaining encouraging results.10–17 It seems that Biohit®’s Ultra-Rapid Urease Test (URUT) is a promising diagnostic method but wider and more diverse evidence is still needed to recommend the systematic use URUT in clinical practice. Therefore, the aim of the present study was to evaluate and compare URUTs diagnostic accuracy for the diagnosis of H. pylori in the Spanish population.

MethodsPatients

In this prospective, blind, multicenter study, a total of 144 patients (68% female, mean age 49 years) who attended digestive services for upper gastrointestinal endoscopy were consecutively enrolled. Inclusion criteria were: patients over 18 years of age suffering from dyspepsia. Exclusion criteria were: presence of hepatic, renal, lung, endocrine, metabolic, haematological or malignant diseases; previous H. pylori eradication treatment; history of alcohol or drug abuse; and pregnancy or nursing.

Proton pump inhibitor (PPI) treatment was not considered an exclusion criterion as, in clinical practice, most patients undergoing upper gastrointestinal endoscopy are taking these drugs prior to the procedure. However, sub-analyses depending on PPI intake were performed for all calculations.

The investigator in charge of each diagnostic test was blinded to the results of the other tests.

Biopsies

Three antrum and one corpus biopsies were obtained. One antrum and the corpus biopsies were used for the standard histological analysis. Biopsies were fixed in 10% formalin and separately embedded in paraffin blocks. The sections, serially cut and stained with haematoxylin & eosin, were examined with light microscopy for the histological assessment of H. pylori infection by a pathologist. One antrum biopsy was used for the diagnosis of H. pylori infection with the traditional RUT. The other biopsy was used for diagnosis with URUT.

Urease kits

RUT was performed using one out of the three most common kits in Spanish hospitals (CLO test®, Jatrox-test® and Gut plus®). Each hospital used the kit of their own routine clinical practice. The kit was checked out during 24h. Biohit®’s URUT (Biohit®, Helsinki, Finland) was checked 1min, 5min, 30min and 60min after the biopsy was included in the kit's media. Flowchart of the patients is present in Fig. 1 (STARD flowchart).

Figure 1.

Patient STARD flowchart.

(0.13MB).
Ethical issues

This study was performed with the approval and follow up by the hospitals’ Ethics Committees. The design and development followed the WMA Helsinki Declaration of 1964 and its revisions and all applicable regulations. All patients signed an informed consent.

Statistical analysis

Mean and standard deviation were calculated for quantitative variables, and percentage and 95% confidence interval (95%CI) for qualitative variables. Sensitivity, specificity, positive and negative predictive values and positive and negative likelihood ratios were calculated for all urease kits. Z-test was used to compare diagnostic success rates. McNemar test was used to compare the distribution of results for both tests. Significance was considered for p<0.05. In order to demonstrate equivalence of RUT and URUT, a sample size of 140 patients was calculated [significance level (alpha) 5%, power (beta) 90% and margin of equivalence (d) 5%].

Results

• Study population characteristics

One hundred and forty-four dyspeptic patients were included in 11 hospitals all over Spain in 2009. Average age was 49, and 69% were women. Forty-two percent of patients were taking PPIs. Fifty percent were H. pylori positive by histology. Mean waiting time for positive RUT result was 6.2h.

• Overall diagnostic success of RUT and URUT

RUT and URUT diagnosis were correct in 85.9% (95%CI=80–92%) (Table 1) and 90.0% (95%CI=85–95%) (Table 2) of the cases respectively.

Table 1.

CrossTable of Rapid Urease Tests (by commercial brand) vs. histology in the diagnosis of H. pylori infection.

    HistologyTotal 
    Negative  Positive   
All kits  Negative  62  12  74 
  Positive  54  61 
  Total  69  66  135 
CLO-test®  Negative  29  32 
  Positive  22  25 
  Total  32  25  57 
Jatrox-test®  Negative  21  25 
  Positive  22  23 
  Total  22  26  48 
Gut-plus®  Negative  11  16 
  Positive  10  14 
  Total  15  15  30 
Table 2.

CrossTable of Ultra Rapid Urease Test (URUT) (by time of assessment) vs. histology in the diagnosis of Helicobacter pylori infection.

    All KitsExcluding Gut-Plus®
    HistologyTotal  HistologyTotal 
    Negative  Positive    Negative  Positive   
URUT 1min  Negative  68  41  109  54  28  82 
  Positive  24  26  21  22 
  Total  70  65  135  55  49  104 
URUT 5min  Negative  67  27  94  54  17  71 
  Positive  41  44  35  36 
  Total  70  68  138  55  52  107 
URUT 30min  Negative  67  15  82  54  10  64 
  Positive  53  57  43  45 
  Total  71  68  139  56  53  109 
URUT 1Negative  67  10  77  54  60 
  Positive  58  62  47  49 
  Total  171  68  139  56  53  109 

• RUT by commercial brand

CLO-test®, Jatrox-test® and Gut-plus® were used for the RUT in 37%, 41% and 22% the patients respectively. Comparing their results with the gold standard, CLO-test® and Jatrox-test® each diagnosed correctly 91% of patients but Gut-plus® only 70% (Z-test=2.55, p<0.01). Therefore sub-analyses excluding those patients diagnosed with Gut-plus® were performed (Table 1). Mean time for positive result was 6.2±9.3h (CLO-test® 9.2h; Jatrox-test® 3.0h; Gut-plus® 7.3h).

• URUT by time checked

One minute after biopsies were placed inside the kit's media, URUT correctly diagnosed H. pylori infection in 68% of patients, 78% at 5min, 86% at 30min and 90% at 60min (Table 2).

• Accuracy depending on PPI intake

All the previous analyses were performed twice, considering all data and excluding those patients under PPI treatment. RUT and URUT (after 60min) diagnosis was correct in 93% and 97% of patients, including only those patients where the traditional RUT was not Gut-Plus® (Table 3).

Table 3.

CrossTable of Rapid and Ultra Rapid Urease Tests (RUT and URUT) vs. histology in the diagnosis of Helicobacter pylori infection in patients not taking proton pump inhibitor treatment.

    All kitsExcluding Gut-plus®
    HistologyTotal  HistologyTotal 
    Negative  Positive    Negative  Positive   
RUTNegative  30  36  27  30 
Positive  38  41  30  31 
  Total  33  44  77  28  33  61 
URUTNegative  32  36  28  29 
Positive  42  45  34  35 
  Total  34  47  81  29  35  64 

• URUT and RUT's accuracy.

Accuracy calculations were performed for RUT and URUT (at checkpoints 30 and 60min). PPI treatment and RUT brand sub-analyses were also studied and data is shown in Table 4. URUT (at 30 and 60min) and RUT's diagnostic successes were not significantly different (Z-test p>0.05). McNemar tests could not demonstrate statistically significant differences when comparing the distribution of success between RUT and URUT.

Table 4.

Rapid and Ultra Rapid Urease Test's (RUT and URUT) accuracy for the diagnosis of Helicobacter pylori infection in all subanalyses.

    Sensitivity  Specificity  PPV  NPV  PLR  NLR 
RUTAll kits  82% (75–88%)  90% (85–95%)  89% (83–94%)  84% (78–90%)  8.6 (4.0–16.4)  0.20 (0.12–0.34) 
No Gut-plus®  86% (80–93%)  94% (90–99%)  94% (89–98%)  88% (82–94%)  16 (5–47)  0.15 (0.07–0.29) 
URUTAll kits  85% (79–91%)  94% (91–98%)  94% (89–98%)  87% (81–93%)  15 (6–39)  0.16 (0.09–0.28) 
No Gut-plus®  89% (83–95%)  96% (93–100%)  96% (92–100%)  90% (84–96%)  25 (6–97)  0.12 (0.06–0.25) 
RUT
No PPI
All kits  86% (79–94%)  91% (84–97%)  93% (87–99%)  83% (75–92%)  10 (3–28)  0.15 (0.07–0.32) 
No Gut-plus®  91% (84–98%)  96% (92–100%)  97% (92–100%)  90% (82–98%)  25 (4–175)  0.09 (0.03–0.28) 
URUT 1h
No PPI
All kits  91% (85–97%)  94% (89–99%)  96% (91–100%)  89% (82–96%)  16 (4–60)  0.09 (0.04–0.24) 
No Gut-plus®  97% (93–100%)  97% (92–100%)  97% (93–100%)  97% (92–100%)  28 (4–193)  0.03 (0–0.2) 

PPV: positive predictive value; NPV: negative predictive value; PLR: positive likelihood ratio; NLR: negative likelihood ratio.

95% confidence interval is shown in parentheses.

Discussion

The present study evaluated the accuracy of RUT and URUT in the diagnosis of H. pylori infection and showed equivalent results for RUT kits (24h result) and Biohit®’s URUT at minutes 30 and 60.

Three RUT kits were studied; Jatrox-test® and CLO-test® offered equivalent results but Gut-plus® had significantly lower accuracy in the studied population. Although this lower accuracy may be due to a low sample size beta error, all the subsequent analyses were calculated both including and excluding patients diagnosed with Gut-plus® kit. Jatrox-test® was able to offer positive results quicker than the other two kits, although the 24h waiting period was still needed in order to avoid false negative results.

The accuracy of Biohit®’s URUT diagnosis at 30min was statistically equivalent to the traditionally used RUT kits. Even though the kit's manual clearly establishes the waiting period in 30min, increasing it to one hour improved sensitivity (better than RUT) in all sub-analyses and did not reduce specificity, thus it may be recommendable to wait 60min before checking the URUT result.

In accordance to previously published data,1,7,8 the intake of PPI drugs the two weeks prior to endoscopy reduced the accuracy of all urease kits in our study. Therefore, the withdrawal of PPIs before endoscopy should be recommended to patients. The results of RUT (Jatrox-test® and CLO-test®) and URUT in our study in patients not taking PPI drugs were excellent, especially in the case of URUT's diagnosis after 60min.

Finally, URUT and RUT kits’ incorrect diagnosis mostly occurred in the same patients and no statistically significant differences were found when comparing the distribution of correct and incorrect diagnosis of both methods. This implies that traditional RUTs limitations regarding diagnostic results,1,4–8 probably due to low bacterial urease activity, are not overcome by URUT.

The main limitation of this study derives from mimicking clinical practice in Spain where there is no systematic implementations of recommendations regarding biopsy collection during endoscopy which did not allow deeper analysis of our patients.

In summary, previous studies comparing diverse ultra rapid urease tests have shown that the accuracy of ultra rapid tests is equivalent to that of traditional ones.10–15 The present study seems to indicate that the URUT version developed by Biohit® is equivalent to the standard RUT used in Spain, reaching approximately 90% sensitivity and specificity in patients naïve to eradication therapy. In general, the use of a validated URUT, as it offers equivalent results than 24h kits, may be recommended in order to start treatment earlier and avoid doubling visits to the outpatient clinic which would reduce overall costs in the management of H. pylori infection and minimize inconveniences to patients.

Conflict of interests

Dr. Gisbert has served as speaker, consultant and advisory member for or has received research funding from Almirall, Nycomed, AstraZeneca, Casen Recordati, and Allergan. Dr. McNicholl has received retribution from Allergan for formative actions. Dr. Castro-Fernandez has received retribution from Allergan for formative actions. Dr. Perez-Aísa has received retribution from Allergan and Mylan for formative actions.

Acknowledgements

CIBERehd is funded by the Instituto de Salud Carlos III. This study was not funded by any Pharmaceutical Company.

Annex
STARD checklist for reporting of studies of diagnostic accuracy

Section and Topic  Item    On page 
TITLE/ABSTRACT/
KEYWORDS 
Identify the article as a study of diagnostic accuracy (recommend MeSH heading ‘sensitivity and specificity’). 
INTRODUCTION  State the research questions or study aims, such as estimating diagnostic accuracy or comparing accuracy between tests or across participant groups. 
METHODS
Participants  The study population: The inclusion and exclusion criteria, setting and locations where data were collected. 
  Participant recruitment: Was recruitment based on presenting symptoms, results from previous tests, or the fact that the participants had received the index tests or the reference standard? 
  Participant sampling: Was the study population a consecutive series of participants defined by the selection criteria in item 3 and 4? If not, specify how participants were further selected. 
  Data collection: Was data collection planned before the index test and reference standard were performed (prospective study) or after (retrospective study)? 
Test methods  The reference standard and its rationale.   
  Technical specifications of material and methods involved including how and when measurements were taken, and/or cite references for index tests and reference standard. 
  Definition of and rationale for the units, cut-offs and/or categories of the results of the index tests and the reference standard.  7-8 
  10  The number, training and expertise of the persons executing and reading the index tests and the reference standard. 
  11  Whether or not the readers of the index tests and reference standard were blind (masked) to the results of the other test and describe any other clinical information available to the readers. 
Statistical methods  12  Methods for calculating or comparing measures of diagnostic accuracy, and the statistical methods used to quantify uncertainty (e.g. 95% confidence intervals). 
  13  Methods for calculating test reproducibility, if done.   
RESULTS
Participants  14  When study was performed, including beginning and end dates of recruitment. 
  15  Clinical and demographic characteristics of the study population (at least information on age, gender, spectrum of presenting symptoms). 
  16  The number of participants satisfying the criteria for inclusion who did or did not undergo the index tests and/or the reference standard; describe why participants failed to undergo either test (a flow diagram is strongly recommended).  9 & 15 
Test results  17  Time-interval between the index tests and the reference standard, and any treatment administered in between. 
  18  Distribution of severity of disease (define criteria) in those with the target condition; other diagnoses in participants without the target condition.  N/A 
  19  A cross tabulation of the results of the index tests (including indeterminate and missing results) by the results of the reference standard; for continuous results, the distribution of the test results by the results of the reference standard.  16-19 
  20  Any adverse events from performing the index tests or the reference standard.  N/A 
Estimates  21  Estimates of diagnostic accuracy and measures of statistical uncertainty (e.g. 95% confidence intervals).  16-19 
  22  How indeterminate results, missing data and outliers of the index tests were handled.  N/A 
  23  Estimates of variability of diagnostic accuracy between subgroups of participants, readers or centers, if done. 
  24  Estimates of test reproducibility, if done.  N/A 
DISCUSSION  25  Discuss the clinical applicability of the study findings.  11-12 

References
[1]
P. Malfertheiner, F. Megraud, C.A. O’Morain, et al.
Management of Helicobacter pylori infection-the Maastricht V/Florence Consensus Report.
[2]
N.J. Talley.
Functional dyspepsia: advances in diagnosis and therapy.
Gut Liver, 11 (2017), pp. 349-357
[3]
J. Guarner, N. Kalach, Y. Elitsur, S. Koletzko.
Helicobacter pylori diagnostic tests in children: review of the literature from 1999 to 2009.
Eur J Pediatr, 169 (2010), pp. 15-25
[4]
F. Mégraud, P. Floch, J. Labenz, P. Lehours.
Diagnostic of Helicobacter pylori infection.
Helicobacter, 21 (2016), pp. 8-13
[5]
C. Ricci, J. Holton, D. Vaira.
Diagnosis of Helicobacter pylori: invasive and non-invasive tests.
Best Pract Res Clin Gastroenterol, 21 (2007), pp. 299-313
[6]
M. Selgrad, A. Kandulski, P. Malfertheiner.
Helicobacter pylori: diagnosis and treatment.
Curr Opin Gastroenterol., 25 (2009), pp. 549-556
[7]
P. Midolo, B.J. Marshall.
Accurate diagnosis of Helicobacter pylori. Urease tests.
Gastroenterol Clin North Am, 29 (2000), pp. 871-878
[8]
J.P. Gisbert, J. Molina-Infante, J. Amador, et al.
IV Spanish Consensus Conference on Helicobacter pylori infection treatment.
Gastroenterol Hepatol, 39 (2016), pp. 697-721
[9]
J.P. Gisbert, V. Abraira.
Accuracy of Helicobacter pylori diagnostic tests in patients with bleeding peptic ulcer: a systematic review and meta-analysis.
Am J Gastroenterol, 101 (2006), pp. 848-863
[10]
D. Vaira, L. Gatta, C. Ricci, et al.
A comparison amongst three rapid urease tests to diagnose Helicobacter pylori infection in 375 consecutive dyspeptic.
Intern Emerg Med, 5 (2010), pp. 41-47
[11]
D. Vaira, N. Vakil, L. Gatta, et al.
Accuracy of a new ultrafast rapid urease test to diagnose Helicobacter pylori infection in 1000 consecutive dyspeptic patients.
Aliment Pharmacol Ther, 31 (2010), pp. 331-338
[12]
K.L. Goh, P.L. Cheah, P. Navaratnam, S.C. Chin, S.D. Xiao.
HUITAI rapid urease test: a new ultra-rapid biopsy urease test for the diagnosis of Helicobacter pylori infection.
[13]
N. van Keeken, E. van Hattum, W.A. de Boer.
Validation of a new, commercially available dry rapid urease test for the diagnosis of Helicobacter pylori infection in gastric biopsies.
Neth J Med, 64 (2006), pp. 329-333
[14]
J.M.K. da Silva-Etto, R. Mattar, C.A. Villares-Lopes, et al.
Evaluation of diagnostic accuracy of two rapid stool antigen tests using an immunochromatographic assay to detect Helicobacter pylori.
Clin Biochem, (2017), pp. 30129-30137
[Epub ahead of print], pii: S0009-9120
[15]
C.A. Tseng, W.M. Wang, D.C. Wu.
Comparison of the clinical feasibility of three rapid urease tests in the diagnosis of Helicobacter pylori infection.
Dig Dis Sci., 50 (2005), pp. 449-452
[16]
N.A. Adu-Aryee, L. Aabakken, F. Dedey, et al.
Comparison of endoscopic based diagnosis with Helicobacter urease test for Helicobacter pylori infection.
BMC Res Notes, 9 (2016), pp. 421
[17]
X. Calvet, J. Sánchez-Delgado, A. Montserrat, et al.
Accuracy of diagnostic tests for Helicobacter pylori: a reappraisal.
Clin Infect Dis, 48 (2009), pp. 1385-1391
Copyright © 2017. Elsevier España, S.L.U.. All rights reserved
Descargar PDF
Opciones de artículo
es en pt

¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

Are you a health professional able to prescribe or dispense drugs?

Você é um profissional de saúde habilitado a prescrever ou dispensar medicamentos