covid
Buscar en
Journal of Applied Research and Technology. JART
Toda la web
Inicio Journal of Applied Research and Technology. JART Carica papaya peel mediated synthesis of silver nanoparticles and its antibacter...
Información de la revista
Vol. 15. Núm. 5.
Páginas 413-422 (octubre 2017)
Compartir
Compartir
Descargar PDF
Más opciones de artículo
Visitas
14153
Vol. 15. Núm. 5.
Páginas 413-422 (octubre 2017)
Original
Open Access
Carica papaya peel mediated synthesis of silver nanoparticles and its antibacterial activity against human pathogens
Visitas
14153
J. Balavijayalakshmi
Autor para correspondencia
, V. Ramalakshmi
Department of Physics, PSGR Krishnammal College for Women, Coimbatore, Tamilnadu, India
Este artículo ha recibido

Under a Creative Commons license
Información del artículo
Resumen
Texto completo
Bibliografía
Descargar PDF
Estadísticas
Figuras (13)
Mostrar másMostrar menos
Tablas (2)
Table 1. Optical band gap value of silver nanoparticles.
Table 2. Zone of inhibition of silver nanoparticles using different concentrations of papaya peel extract.
Mostrar másMostrar menos
Abstract

Metallic nanoparticles are traditionally synthesized by wet chemical techniques, in which the chemicals used are quite often toxic and flammable. Ripe carica papaya peel is found to be a suitable source for green synthesis of silver nanoparticles. In the present work, a cost effective and environmental friendly technique for the green synthesis of silver nanoparticles from 1mM silver nitrate (AgNO3) solution through the extract of ripe Carica papaya peel of various concentrations (5ml, 10ml, 15ml, 20ml, 25ml) is described. The synthesized silver nanoparticles are characterized by using the UV–vis absorption spectroscopy, FT-IR, XRD, SEM and TEM. The formation of silver nanoparticles is confirmed by surface plasmon resonance, determined by UV–vis spectra at 400–435nm. The shift in the absorption bands and variation in the calculated optical band gaps for the various concentrations of papaya peels extracts are also observed. The FT-IR spectra reveal that an increase in the concentration of the papaya peel extract shifts the bands to higher wavelengths. The average crystallite size for various concentrations of papaya peel extract is observed from XRD spectral analysis and is found to be around 16–20nm, which is in good agreement with the TEM analysis. The SEM analysis shows the spherical structure of the silver nanoparticles with some agglomeration for higher concentrations of papaya peel extract. The synthesized silver nanoparticles show good antibacterial activity against human pathogens such as Escherichia coli and Staphylococcus aureus and it has many medical applications.

Keywords:
Silver nanoparticles
UV–vis
FT-IR
XRD
SEM
Antibacterial activity
Texto completo
1Introduction

The recent research based on noble metal nanoparticles (silver, gold, etc.) has become more focussed, due to the uniqueness of the optical, electrical, mechanical, magnetic, size dependent, chemical properties of these nanoparticles. The nanoparticles are very much different from those of the bulk materials (Forough & Fahadi, 2011). Due to these size dependent properties, metal nanoparticles have significant applications in electronics, optoelectronics, magnetic, biomedical and information storage systems (Banerjee, Satapathy, Mukhopahayay, & Das, 2014). A number of approaches are available for the synthesis of metal nanoparticles, such as chemical, electrochemical, photochemical and radiation. The chemical method produces toxic chemicals that may have an adverse effect in medical applications. Hence there is a need for biosynthesis of nanoparticles. The biosynthesis of metal nanoparticles is a widely accepted technology and it is a kind of bottom up approach where the main mechanism behind is reduction. The nanoparticles produced by this method are safe, cost effective and more environmental friendly when compared to the chemical methods (Johnson & Prabu, 2015). Compare to other genial biological processes, the use of agricultural waste like peel extracts for the production of nanoparticles is expedient. The fruit peels are especially easily available, efficient, affordable, eco-friendly, natural and also very rich in bioactive compound. These bioactive compounds can be used as antioxidants and antimicrobial agents, causing most researchers to aim at identifying an efficient way to extract these bioactive compounds from the fruit peels.

Silver nanoparticles (AgNPs) play a profound role in biosynthesis because of their distinctive properties such as good conductivity, chemical stability, catalytic and antibacterial activity (Vadlapudi, Kaladhar, Behara, Naidu, & Sujatha, 2013). Silver nanoparticles have strong inhibitory and bacterial effects, which have been used to prevent and treat various diseases (Veerasamy et al., 2011). Silver nanoparticles have been assorted into more than 200 consumer products, including clothing, medicines and cosmetics due to its medicinal and antimicrobial properties. The papaya, papaw, or pawpaw is the fruit of the plant Carica papaya, the only species in the genus Carica of the plant family Caricaceae. It is native to the tropics of the Americas but has now spread all over the world. The fresh papaya fruit has attractive striking odours, high vitamin content (i.e., vitamin A and C) and high fibre content. The other parts of papaya such as skin, pulp, seeds, stems and leaves also contain high levels of proteins, vitamins and variety of phytochemicals, including natural phenols which are used in the elaboration of cosmetics and medications (Aravind, Bhowmik, Duraivel, & Harish, 2013; Khan, Yadav, Srivastava, & Pal, 2012; Kokila, Ramesh, & Geetha, 2016).

Here, we report facile and cost effective biogenic synthesis of Ag NPs using aqueous extract of carica papaya peel and investigation of the antimicrobial of the synthesized Ag NPs.

2Materials and methods

The carica papaya fruit is collected from the local market in Coimbatore and the chemical silver nitrate is purchased from Hi-media.

2.1Preparation of peel extract

The papaya peels are washed thoroughly with distilled water to remove visible dust particles and air dried at room temperature to remove the water molecules.

About 25g of dried and finely sliced peels are taken and added into 100ml of distilled water and heated for 30min. The crude extract is then filtered out using Whatman No. 1 filter paper to get a clear bio extract (Fig. 1). Silver nanoparticles are synthesized from silver nitrate by using aqueous bio extract of carica papaya peels as reducing agent.

Fig. 1.

Carica papaya peel extract.

(0.12MB).
2.2Active constituents and synthesis mechanisms of silver nanoparticles

Papaya peel has various photochemicals especially, phenolic compounds which have antioxidant properties. The two important biologically active compounds in carica papaya are chymopapain and papain, which are extensively used for digestive disorders (Huet et al., 2006). Carica papaya derived papain, caricain, chymopain, and glycerine endopeptidase can improve acidic pH conditions and pepsin degradation. Lipase, or CPL and ahydrolase are the other active compounds of Carica Papaya, which are firmly bonded to the water-insoluble fraction of crude papain. This is considered as a “naturally immobilized” biocatalyst (De María, Sinisterra, Tsai, & Alcántara, 2006; Kokila et al., 2016).

The papaya peel extracts are rich in vitamins, amino acids, carbohydrates, β-carotene, lycopene and polyphenols. Papaya peel extracts acts as an ace source of riboflavin which helps to form the bound co-enzyme namely, Falvin Mono Nucleotide (FMN) and flavin adenine dinucleotide (FAD), which acts as a catalyst for various reduction and oxidation reactions. The other possible mechanisms for the synthesis of silver nanoparticles are nitrate reductases and shuttle quinine process (Durán, Marcato, Alves, De Souza, & Esposito, 2005; Kokila et al., 2016). In this study, the similar mechanism is carried out for the synthesis of silver nanoparticles using the extract of carica papaya.

2.3Preparation of silver nanoparticles

About 5ml of filtered bio peel extract is taken and added into 1mM of pure aqueous silver nitrate solution and stirred for an hour for the reduction of silver nitrate into silver nanoparticles. The reduction process Ag+ to Ag0 nanoparticles is followed by the colour change of the solution from yellow to brownish-yellow to deep brown depending on parameters studied such as the extract concentration. The formation of brown colour confirms the syntheses of silver nanoparticles are completed. A similar method is followed for the preparation of silver nanoparticles by using various concentrations of the carica papaya peel extract (Fig. 2).

Fig. 2.

Preparation of silver nanoparticles.

(0.09MB).
3Results and discussion3.1Visible observation

Figure 3(a–e) shows the mixture of synthesized silver nanoparticles using papaya peel extracts of various concentrations (5ml, 10ml, 15ml, 20ml and 25ml). The colour of the reaction mixture after 1h from the initiation of the reaction is different for different concentrations of carica papaya peel extract as evidenced from Figure 3. The colour of the reaction mixture for the 5ml concentration of papaya peel extract shows a reddish brown colour, which increases with increase in the peel extract concentration from 5ml to 25ml.

Fig. 3.

Silver nanoparticles using (a) 5ml, (b) 10ml, (c) 15ml, (d) 20ml, (e) 25ml of carica papaya peel extract after 1h of incubation.

(0.16MB).

The maximum colour intensity (deep brown) is observed for the 15ml concentration of papaya peel extract. The intensity of the colour is found to decrease (shallow brown) with the increase in the extract concentration from 20 to 25ml. The deep brown colour of the reaction mixtures indicates the formation of more number of silver nanoparticles for the concentrations up to 15ml of papaya peel extract. Similarly, the shallow brown colour of the reaction mixtures indicate the formation of less number of silver nanoparticles for the concentrations above 15ml of papaya peel extract. It is observed that the synthesized silver nanoparticles are aggregated for the higher concentration of papaya peel extract. The aggregation of silver nanoparticles leads to destabilization of silver nanoparticles (Mohapatra, Kaintura, Singh, Kuriakose, & Mohapatra, 2015).

Figure 4 shows the reaction mixture of silver nanoparticles after 24h of incubation from the initiation of reaction at room temperature. It is observed that the colour of all the reaction mixtures becomes more intense (deep brown), thereby confirming the formation of more number of silver nanoparticles, as the reaction time increases exponentially.

Fig. 4.

Visible observation of silver nanoparticles using (a) 5m, (b) 10ml, (c) 15ml, (d) 20ml, (e) 25ml of papaya peel extract after 24h of incubation.

(0.15MB).
3.2UV–visible spectral analysis

Figure 5 shows the UV–visible absorption spectra of silver nanoparticles with five different concentrations (5ml, 10ml, 15ml, 20ml and 25ml) of the aqueous papaya peel extract at room temperature after 24h of incubation. The absorption (or) surface plasmon resonance (SPR) band for 5ml, 10ml, 15ml, 20ml and 25ml concentrations of papaya peel mediated silver nanoparticles are observed at 410nm, 420nm, 435nm, 422nm, 418nm respectively. The SPR peak with maximum and minimum intensity is observed at 435nm and 418nm for 15ml and 25ml concentration of papaya peel extract respectively (Mohapatra et al., 2015).

Fig. 5.

UV–visible spectra of silver nanoparticles using (a) 5ml, (b) 10ml, (c) 15ml, (d) 20ml, (e) 25ml of papaya peel extract.

(0.11MB).

From Figure 5, it is also observed that the absorption peak wavelength of AgNPs shifts to the higher wavelength side as the concentration of the papaya peel extract increases from 5 to 15ml, corresponding to the red shift. This red shift indicates a gradual increment in the mean diameter of the silver nanoparticles. It is further observed that, as the concentration of papaya peel extract increases to 20 and 25ml, the absorption wavelength of AgNPs shifts to lower wavelength side corresponding to blue shift. This blue shift indicates a gradual reduction in the mean diameter of the silver nanoparticles.

The optical band gap value for the various concentrations of carica papaya peel extracts are calculated and are listed in Table 1. The optical band gap decreases as the concentration of papaya peel extract increases from 5 to 15ml, which confirms that the size of the particle increases and thereby the energy band gap decreases. On further increase in the concentration of papaya peel extract to 20ml and 25ml, the optical band gap is found to increase thereby confirming the decrease in the size of the silver nanoparticles for these concentrations, which could also be evidenced from XRD analysis (Ghobadi, 2013; Kamat, Meisel, Weller, & Eychmuller, 1996; Schön & Simon, 1995).

Table 1.

Optical band gap value of silver nanoparticles.

Sample  Optical band gap (eV) 
5ml  4.9 
10ml  4.7 
15ml  4.6 
20ml  4.7 
25ml  4.8 
3.3Time dependent UV–visible spectral analysis

The time dependent UV–visible spectra of silver nanoparticles with different concentrations (5ml, 10ml, 15ml, 20ml, and 25ml) of papaya peel extract is shown in Figure 6. The UV–visible spectra of silver nanoparticles are recorded after time intervals of 0min, 15min, 30min, 45min, 60min and 24h from the initiation of reaction. It is observed that the incubation period of the reaction mixture shows a gradual increase in absorbance spectrum with surface resonance Plasmon resonance band. It is observed that the absorption peak intensity is lower for 0min of reaction time, which confirms that less number of silver nanoparticles is formed in the reaction mixture. The absorption peak intensity gradually increases with increase in the reaction time from 0min to 60min for all the concentrations of papaya peel extract, which indicates an increase in the concentration of silver nanoparticles in the reaction mixture (Mohapatra et al., 2015). The highest absorption peak intensity is observed after 24h of incubation from the initiation of the reaction, which indicates the complete formation of silver nanoparticles in all the reaction mixtures.

Fig. 6.

Time dependent UV–visible spectra of silver nanoparticles using (a) 5ml, (b) 10ml, (c) 15ml, (d) 20ml, (e) 25ml concentration of peel extract.

(0.51MB).
3.4FT-IR analysis

The FT-IR transmission spectra of the carica papaya peel extract is shown in Figure 7. The absorption bands around 2924.09cm−1, 2862.36cm−1 and 1458.18cm−1, 979.84cm−1 may correspond to stretching or bending vibrations of C–H alkenes. The bands around 3726.47cm−1and 918.12cm−1 respectively are attributed to stretching and bending vibrations of carboxylic acids. The bands around 1188.15cm−1 and 1658.78cm−1 may be assigned to amide I and II N–H bending arising due to carbonyl stretching and peptide linkages of proteins respectively (Khalil, Ismail, El-Baghdady, & Mohamed, 2014; Venkatesan, Subramanian, Tumala, & Vellaichamy, 2014). From the FT-IR analysis, the presence of carboxyl and amide groups in the carica papaya peel extracts is confirmed. These groups are responsible for the bio-reduction of silver ions into silver nanoparticles.

Fig. 7.

FT-IR spectrum of papaya peel extract.

(0.07MB).

Figure 8 shows the FT-IR spectra of carica papaya peel mediated silver nanoparticles (AgNPs), for various concentrations (5ml, 10ml, 15ml, 20ml and 25ml) of papaya peel extract. The FT-IR spectra show characteristic absorption bands around 700–750cm−1, 950–980cm−1, 1000–1150cm−1, 1200–1300cm−1, 1330–1370cm−1, 1500–1550cm−1, 1600–1650cm−1 and 3100–3400cm−1. The bands around 700–750cm−1 may be attributed to stretching vibrations of C–Cl alkyl halides (Khalil et al., 2014). The observed bands around 950–980cm−1 may arise due to bending vibrations of C–H group alkenes. The absorption band around 1000–1150cm−1 may be assigned to C–O stretching vibrations of carboxylic acids. The bands around 1200–1300cm−1 are associated with N–H bend amines (Sivakumar, Nethradevi, & Renganathan, 2012). The bands around 1330–1370cm−1 may be due to C–N stretch amine group. The bands around 1500–1550cm−1 correspond to N–O asymmetric stretch nitro compounds. The bands around 1600–1650 may correspond to stretching vibrations of primary and secondary amines (Khalil et al., 2014; Shanmugavadivu, Kuppusamy, & Ranjithkumar, 2014; Sivakumar et al., 2012). The bands around 3100–3400cm−1may be due to CO stretch of carboxylic acids (Khalil et al., 2014).

Fig. 8.

FT-IR spectra of silver nanoparticles using (a) 5ml, (b) 10ml, (c) 15ml, (d) 20ml, (e) 25ml of papaya peel extract.

(0.11MB).

The FT-IR spectrum of silver nanoparticles is shown in Figure 8, confirms the presence of carboxyl group and amine group in the reaction mixture by the presence of the appropriate bands. It is observed that on increasing the concentration of papaya peel extracts, the bands are shifted to higher wavelength. It is further observed on comparing Figures 7 and 8, that the bands around 3100–3400cm−1 and 1600–1650cm−1, due to carboxylic and amine groups respectively, are shifted to higher wavelengths due to the binding of silver ions when compared with respect to pure carica papaya peel extract and the depth of the band decreases (Khalil et al., 2014; Sivakumar et al., 2012). These carboxyl and amide groups indicate the presence of secondary amines, which is a signature marker of proteins, thus confirming the bio-fabrication of silver nanoparticles, by the action of the proteins or phytochemicals. Therefore, it may be confirmed that, these bio-molecules are responsible for the capping and stabilization of the synthesized nanoparticles. The FT-IR analysis suggests that proteins are involved in the capping and stabilization of the synthesized silver nanoparticles.

3.5XRD structural analysis

The XRD patterns of bio-synthesized silver nanoparticles of various concentrations of papaya peel extract are shown in Figure 9. The XRD patterns clearly show that, the silver nanoparticles formed by the bio reduction of silver ions by papaya peel broth are crystalline in nature. XRD analysis is used to determine the phase distribution, crystallinity and purity of the synthesized silver nanoparticles.

Fig. 9.

XRD spectra of silver nanoparticles using (a) 5ml, (b) 25ml, (c) 20ml, (d) 10ml, (e) 15ml of papaya peel extract.

(0.13MB).

The XRD patterns of silver nanoparticles show five distinct peaks in the spectrum of 2θ value ranging from 30° to 80°. The peaks are indexed with reference to the standard JCPDS card no. 04-783 for silver and are found to be (111), (200), (222), (220) and (311), corresponding to the cubic face of silver nanoparticles (Saravanakumar, Ganesh, Jayaprakash, & Jang, 2015). The peaks observed around 27° and 32° may due to the peel extract. These Braggs peak might have resulted due to capping agent, stabilizing the nanoparticles (Ibrahim, 2015; Roopan et al., 2013). The crystallite sizes of the silver nanoparticles using various concentrations of papaya peel extract are calculated using the Debye–Scherrer's equation (Gnanajobitha, Rajeshkumar, Kannan, & Annadurai, 2013).

The average crystallite size of silver nanoparticles synthesized using 5ml, 10ml, 15ml, 20ml and 25ml of papaya peel extract are found to be 16.1nm, 16.3nm, 17.9nm, 17.8nm, 17.7nm respectively. It is observed that the average crystallite size of the particles increases with increase in the concentration of papaya peel extract from 5ml to 15ml and with further increase in the concentration to 20ml and 25ml, the crystallite size of the particles decreases gradually. The decrease in crystallite size of the particle could also be confirmed from the UV–visible spectral analysis. It is further observed that the crystallite size obtained from the synthesized silver nanoparticles is much smaller than that reported by Kokila et al. in their article as 28nm and this reduction in crystallite size enhance the properties of silver nanoparticles.

3.6SEM analysis

Scanning electron microscopy (SEM) analysis is performed for studying the surface morphology and shapes of silver nanoparticles. Figure 10(a–e) shows the SEM images of various magnifications of silver nanoparticles synthesized using 5ml, 10ml, 15ml, 20ml and 25ml concentrations of carica papaya peel broth respectively. It is observed that the silver nanoparticles are spherical in shape and the concentration of the extract alters the size and shape of nanoparticles. The particles are uniformly distributed and no aggregations are observed for 5–15ml of peel extract. But the particles get agglomerated as the concentration of papaya extract increases above 15ml as can be evidenced from Figure 10(d–e). The agglomeration of particle leads to destabilization of silver nanoparticles (Narmadha, Hemashenbagam, Sathiya Vimal, & VasanthaRaj, 2013).

Fig. 10.

SEM images of silver nanoparticles using (a) 5ml, (b) 10ml, (c) 15ml, (d) 20ml, (e) 25ml of papaya peel extract.

(0.91MB).
3.7TEM analysis

The TEM images of silver nanoparticles synthesized using aqueous extract of carica papaya peel are shown in Figure 11. The concentration of the extract alters the shape and size of silver nanoparticles in the solution. These images suggest that the morphology of the nanoparticles is mostly spherical in shape. A few agglomerated nanoparticles are also observed in some places, thereby indicating possible sedimentation at a later time. The average particle size of silver nanoparticles is found to be from 15nm to 20nm. The results are in good agreement with the crystallite size obtained from XRD analysis.

Fig. 11.

TEM images of silver nanoparticles using papaya peel extract.

(0.91MB).
3.8Saed analysis

Figure 12 shows the selected area electron diffraction pattern (SAED) of the silver nanoparticles synthesized using carica papaya peel extract as reducing agent. The ring patterns indicate that the particles are crystalline in nature. Each ring corresponds to the different lattice planes obtained from the XRD analysis.

Fig. 12.

SAED patterns of silver nanoparticles using papaya peel extract.

(0.15MB).
3.9Antibacterial activity

Antibacterial property of silver nanoparticles synthesized using five different concentrations of papaya peel extract against two different human pathogens Escherichia coli (gram negative) and Staphylococcus aureus (gram positive) are shown in Figure 13. The labels S, D, P, 50 and 100 represent the inhibition zones for standard antibiotic, distilled water, pure carica papaya peel extract, 50μl AgNPs and 100μl AgNPs respectively. It is observed that, the antimicrobial activity of silver nanoparticles with different papaya peel extracts is found to be significant against both the bacterial strains, while the plant extract and distilled water does not show any such activity.

Fig. 13.

Antibacterial activities of silver nanoparticles against (a) Escherichia coli (b) Staphylococcus aureus.

(0.34MB).

The inhibition zones for the silver nanoparticles with different concentrations of papaya peel extract are listed in Table 2. Ampicillin is used as a standard antibiotic to compare the results with the zone obtained using silver nanoparticles. The zone obtained using 100μl of silver nanoparticles is greater than the zone formed using 50μl of silver nanoparticles. It is observed from Figure 13 that, increase in the concentration of silver nanoparticles will result in the formation of a well defined inhibition zone. It is evident from Table 2 that the inhibition zones for both the bacterial strains depend on the concentration of papaya peel extract. A gradual increment in the diameter of the inhibition zone is observed for silver nanoparticles prepared using 5ml, 10ml and 15ml concentrations of papaya extract. This may be due to the increase in the concentration of silver nanoparticles in the reaction mixture. Further, increase in the concentrations of the peel extracts to 20ml and 25ml, the diameter of the inhibition zone obtained for E. coli and S. aureus strains get decreased. The decrease in the diameter of the inhibition zone for high concentration peel extract silver nanoparticles may be due to the presence of low concentration of silver nanoparticles in the reaction mixture. It is confirmed that the antimicrobial activity of silver nanoparticles strongly depends on the concentration of the silver nanoparticles present in the reaction mixture. The anti microbial activity for gram negative bacteria is greater than that for gram positive bacteria for all the concentrations of peel extracts (Banala, Nagati, & Karnati, 2015; Ibrahim, 2015; Saravanakumar et al., 2017). It is further evident that the best results have been achieved in our study, that the zone of inhibition has been found to be 0.75cm (75mm) for E. coli and 0.65cm (65mm) for S. aureus for 100μl is very much higher than that reported by Kokila et al. as 15mm for E. coli and 10mm for S. aureus in their study.

Table 2.

Zone of inhibition of silver nanoparticles using different concentrations of papaya peel extract.

Name of human pathogens  Standard (ampicillin) (30μl)  Zone of inhibition of AgNPs with different concentration of extract
    5ml10ml15ml20ml25m l
    50μl  100μl  50μl  100μl  50μl  100μl  50μl  100μl  50μl  100μl 
E. coli  14  0.4  0.45  0.5  0.55  0.65  0.75  0.55  0.65  0.45  0.5 
S.aureus  15  0.3  0.45  0.5  0.55  0.55  0.65  0.5  0.55  0.45  0.5 
4Conclusion

The present study describes the green synthesis of silver nanoparticles using various concentrations (5ml, 10ml, 15ml, 20ml and 25ml) of carica papaya peel extract as the reducing agent. These nanoparticles are environmental friendly and completely safe. The UV visible spectra of silver nanoparticles show absorption peaks around 400–435nm for the different concentrations of the papaya peel extract. It is observed that on increasing the concentration of the peel extract from 5ml to 15ml, the absorption peaks are being red shifted and the optical band gap is found to decrease, which implies that there is an increase in the particle size. It is further observed that, with increase in concentration of peel the extract to 20ml and 25ml, the absorption peaks are being blue shifted and the optical band gap is increased, which implies that there will be a decrease in the particle size. These variations in particle size could also be confirmed from XRD analysis. The FT-IR spectra confirm the presence of carboxyl group and amine group in the reaction mixture by showing the appropriate bands, which are responsible for the reduction of silver ions into silver nanoparticles. It is also observed that with increase in concentration of the papaya peel extract from 5ml to 25ml, the FT-IR absorption bands are shifted to higher wavelengths because of more interaction of silver ions with reducing agents. XRD spectra reveal that the silver nanoparticles are crystalline in nature and the average crystallite sizes are found to be from 16nm to 18nm which is in good agreement with TEM analysis. SEM micrographs show the spherical shape of the nanoparticles and also the agglomeration for concentrations of extract above 15ml. The antibacterial activity of silver nanoparticles was tested against two bacterial strains, E. coli and S. aureus. The zone of inhibition is found to be greater for gram negative bacteria than gram positive bacteria. The maximum zone of inhibition is obtained for the 15ml concentration of peel extract. This may due to the presence of more number of silver nanoparticles in the reaction mixture which could also be evidenced from UV–vis analysis. It may be concluded from the different characteristic analyses that the nanoparticles synthesized using 15ml concentration of extract are more effective compared to the other concentrations of papaya peel extract.

Conflict of interest

The authors have no conflicts of interest to declare.

References
[Aravind et al., 2013]
G. Aravind, D. Bhowmik, S. Duraivel, G. Harish.
Traditional and medicinal uses of Carica papaya.
Journal of Medicinal Plants Studies, 1 (2013), pp. 7-15
[Banala et al., 2015]
R.R. Banala, V.B. Nagati, P.R. Karnati.
Green synthesis and characterization of Carica papaya leaf extract coated silver nanoparticles through X-ray diffraction, electron microscopy and evaluation of bactericidal properties.
Saudi Journal of Biological Sciences, 22 (2015), pp. 637-644
[Banerjee et al., 2014]
P. Banerjee, M. Satapathy, A. Mukhopahayay, P. Das.
Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: Synthesis, characterization, antimicrobial property and toxicity analysis.
Bioresources and Bioprocessing, 1 (2014), pp. 1-10
[De María et al., 2006]
P.D. De María, J.V. Sinisterra, S.W. Tsai, A.R. Alcántara.
Carica papaya lipase (CPL): An emerging and versatile biocatalyst.
Biotechnology Advances, 24 (2006), pp. 493-499
[Durán et al., 2005]
N. Durán, P.D. Marcato, O.L. Alves, G.I. De Souza, E. Esposito.
Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains.
Journal of Nanobiotechnology, 3 (2005), pp. 1-7
[Forough and Fahadi, 2011]
M. Forough, K. Fahadi.
Biological and green synthesis of silver nanoparticles.
Turkish Journal of Engineering and Environmental Sciences, 34 (2011), pp. 281-287
[Ghobadi, 2013]
Nader Ghobadi.
Band gap determination using absorption spectrum fitting procedure.
International Nano Letters, 3 (2013), pp. 2-4
[Gnanajobitha et al., 2013]
G. Gnanajobitha, S. Rajeshkumar, C. Kannan, G. Annadurai.
Preparation and characterization of fruit-mediated silver nanoparticles using pomegranate extract and assessment of its antimicrobial activity.
Journal of Environmental Nanotechnology, 2 (2013), pp. 04-10
[Huet et al., 2006]
J. Huet, Y. Looze, K. Bartik, V. Raussens, R. Wintjens, P. Boussard.
Structural characterization of the papaya cysteine proteinases at low pH.
Biochemical and Biophysical Research Communications, 341 (2006), pp. 620-626
[Ibrahim, 2015]
H.M. Ibrahim.
Green synthesis and characterization of silver nanoparticles using banana peel extract and their antimicrobial activity against representative microorganisms.
Journal of Radiation Research and Applied Sciences, 8 (2015), pp. 265-275
[Johnson and Prabu, 2015]
I. Johnson, H.J. Prabu.
Green synthesis and characterization of silver nanoparticles by leaf extracts of Cycas circinalis, Ficus amplissima, Commelina benghalensis and Lippia nodiflora.
International Nano Letters, 5 (2015), pp. 43-51
[Kamat et al., 1996]
P.V. Kamat, D. Meisel, H. Weller, A. Eychmuller.
Semiconductor nanoclusters – Physical, chemical, and catalytic aspects.
1st ed., Elsevier Science, (1996), pp. 103
[Khalil et al., 2014]
M.M.H. Khalil, E.H. Ismail, K.Z. El-Baghdady, D. Mohamed.
Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity.
Arabian Journal of Chemistry, 7 (2014), pp. 1131-1139
[Khan et al., 2012]
J.A. Khan, J. Yadav, Y. Srivastava, P.K. Pal.
Invitro evaluation of antimicrobial properties of Carica papaya.
International Journal of Biology, Pharmacy and Allied Sciences, 1 (2012), pp. 933-945
[Kokila et al., 2016]
T. Kokila, P.S. Ramesh, D. Geetha.
Biosynthesis of AgNPs using Carica papaya peel extract and evaluation of its antioxidant and antimicrobial activities.
Ecotoxicology and Environmental Safety, 134 (2016), pp. 467-473
[Mohapatra et al., 2015]
B. Mohapatra, R. Kaintura, J. Singh, S. Kuriakose, S. Mohapatra.
Biosynthesis of high concentration, stable aqueous dispersions of silver nanoparticles using Citrus limon extract.
Advanced Materials Letters, 6 (2015), pp. 228-234
[Narmadha et al., 2013]
E. Narmadha, N. Hemashenpagam, S. Sathiya Vimal, S. Vasantha Raj.
Characterization of papaya fruit mediated silver nanoparticles and evaluation of its antimicrobial and wound healing activity.
International Research Journal of Pharmacy, 4 (2013), pp. 166-169
[Roopan et al., 2013]
S.M. Roopan, G. Rohit Madhumitha, A. Rahuman, C. Kamaraj, A. Bharathi, T.V. Surendra.
Low-cost and eco-friendly phyto-synthesis of silver nanoparticles using Cocos nucifera coir extract and its larvicidal activity.
Industrial Crops and Products, 43 (2013), pp. 631-635
[Saravanakumar et al., 2015]
A. Saravanakumar, M. Ganesh, J. Jayaprakash, H.T. Jang.
Biosynthesis of silver nanoparticles using Cassia tora leaf extract and its antioxidant and antibacterial activities.
Journal of Industrial and Engineering Chemistry, 28 (2015), pp. 277-281
[Saravanakumar et al., 2017]
A. Saravanakumar, M.M. Peng, M. Ganesh, J. Jayaprakash, M. Mohankumar, H.T. Jang.
Low-cost and eco-friendly green synthesis of silver nanoparticles using Prunus japonica (Rosaceae) leaf extract and their antibacterial, antioxidant properties.
Artificial Cells, Nanomedicine, and Biotechnology, 45 (2017), pp. 1165-1171
[Schön and Simon, 1995]
G. Schön, U. Simon.
A fascinating new field in colloid science: Small ligand-stabilized metal clusters and possible application in microelectronics.
Colloid & Polymer Science, 273 (1995), pp. 101-117
[Shanmugavadivu et al., 2014]
M. Shanmugavadivu, S. Kuppusamy, R. Ranjithkumar.
Synthesis of pomegranate peel extract mediated silver nanoparticles and its antibacterial activity.
Open Journal of Advanced Drug Delivery, 2 (2014), pp. 174-182
[Sivakumar et al., 2012]
P. Sivakumar, C. Nethradevi, S. Renganathan.
Synthesis of silver nanoparticles using Lantana camara fruit extract and its effect on pathogens.
Asian Journal of Pharmaceutical and Clinical Research, 5 (2012), pp. 97-101
[Vadlapudi et al., 2013]
V. Vadlapudi, D.S.V.G.K. Kaladhar, M. Behara, G.K. Naidu, B. Sujatha.
Green synthesis of silver and gold nanoparticles.
International Journal of Chemical Studies, 1 (2013), pp. 22-31
[Veerasamy et al., 2011]
R. Veerasamy, T.Z. Xin, S. Gunasagaran, T.F.W. Xiang, E.F.C. Yang, N. Jeyakumar, et al.
Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities.
Journal of Saudi Chemical Society, 15 (2011), pp. 113-120
[Venkatesan et al., 2014]
B. Venkatesan, V. Subramanian, A. Tumala, E. Vellaichamy.
Rapid synthesis of biocompatible silver nanoparticles using aqueous extract of Rosa damascena petals and evaluation of their anticancer activity.
Asian Pacific Journal of Tropical Medicine, 7 (2014), pp. S294-S300

Peer Review under the responsibility of Universidad Nacional Autónoma de México.

Copyright © 2017. Universidad Nacional Autónoma de México, Centro de Ciencias Aplicadas y Desarrollo Tecnológico
Descargar PDF
Opciones de artículo