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Inicio Revista Clínica de Periodoncia, Implantología y Rehabilitación Oral Cuantificación de bacterias relacionadas con la caries dental en saliva de adul...
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Vol. 6. Núm. 2.
Páginas 71-74 (agosto 2013)
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Vol. 6. Núm. 2.
Páginas 71-74 (agosto 2013)
DOI: 10.1016/S0718-5391(13)70124-1
Open Access
Cuantificación de bacterias relacionadas con la caries dental en saliva de adultos y adultos mayores
Quantification of caries-associated bacteria from saliva of adults and older adults
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R.A. Giacaman1,
Autor para correspondencia
giacaman@utalca.cl

Correspondencia autor:
, C. Muñoz-Sandoval1, E. Bravo González1, P. Farfán-Cerda1
1 Unidad de Cariología. Departamento de Rehabilitación Buco-Máxilofacial, Universidad de Talca. Talca, Chile
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Resumen
Introducción

Se considera que Streptococcus mutans (S. mutans) y Lactobacillus spp. se asocian con la caries. Otras especies del biofilm oral, como Streptococcus sanguinis (S. sanguinis) han sido sindicadas como protectoras, pero principalmente en niños. Existe escasa evidencia sobre el nivel de estas bacterias en adultos mayores.

Objetivo

Determinar si existen diferencias en los recuentos microbianos de tres especies relacionadas con la caries en pacientes adultos y adultos mayores.

Materiales y Métodos

Una muestra de pacientes por conveniencia compuesta de 63 pacientes (18 a 79 años) proporcionó saliva estimulada con la que se sembraron placas de agar MSB, MM10 SB y Agar Rogosa para el cultivo de S. mutans, S. sanguinis y Lactobacillus spp., respectivamente. Los recuentos bacterianos fueron expresados como UFC/mL.

Resultados

Los recuentos de S. mutans y Lactobacillus spp. no mostraron variaciones relacionadas con la edad (p>0.05). Los adultos mostraron mayores recuentos de S. sanguinis que los adultos mayores, 3.7 x 105±3.8 × 105UFC/mL y 5.9 × 104±9.4 × 104UFC/mL, respectivamente (p<0.05).

Conclusiones

La edad no parece afectar los niveles de especies tradicionalmente consideradas como cariogénicas. Estos resultados sugieren que la edad puede relacionarse con los patrones de colonización de S. sanguinis en el biofilm oral.

Palabras clave:
S. mutans
S. sanguinis
Lactobacillus spp.
adultos mayores
caries
biofilm oral
Abstract
Introduction

Streptococcus mutans (S. mutans) and Lactobacillus spp. have been traditionally associated with caries, regardless of the subject's age. Other oral biofilm species have been linked as caries protective, including Streptococcus sanguinis (S. sanguinis), but mainly in children. Scarce evidence exists on the levels of these bacteria in older adults.

Aim

To determine whether there are differences in the microbial counts of three caries-associated bacterial species in adults and older adults.

Methodology

A convenience sample of sixty three patients, aged 18 to 79 years, participated in the study. Stimulated saliva samples were obtained and in MSB, MM10 and Rogosa agar plates for the culture of S. mutans, S. sanguinis and Lactobacillus spp., respectively. Bacterial counts were obtained by microscopic observation (10x) of the colonies and expressed in CFU/mL.

Results

Bacterial counts of S. mutans and Lactobacillus spp. did not reveal age-related differences (p>0.05). Adults showed higher S. sanguinis counts than older adults with 3.7 x 105±3.8 × 105CFU/mL and 5.9 × 104±9.4 × 104CFU/mL, respectively (p<0.05).

Conclusions

Age does not seem to affect the levels of bacterial species traditionally associated with caries. The results suggest that age may be related to colonization patterns of S. sanguinis in the oral biofilm.

Key words:
S. mutans
S. sanguinis
Lactobacillus spp.
older adults
caries
oral biofilm
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