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Inicio Revista Clínica de Periodoncia, Implantología y Rehabilitación Oral Cuantificación de bacterias relacionadas con la caries dental en saliva de adul...
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Vol. 6. Núm. 2.
Páginas 71-74 (agosto 2013)
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Vol. 6. Núm. 2.
Páginas 71-74 (agosto 2013)
DOI: 10.1016/S0718-5391(13)70124-1
Open Access
Cuantificación de bacterias relacionadas con la caries dental en saliva de adultos y adultos mayores
Quantification of caries-associated bacteria from saliva of adults and older adults
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R.A. Giacaman1,
Autor para correspondencia
giacaman@utalca.cl

Correspondencia autor:
, C. Muñoz-Sandoval1, E. Bravo González1, P. Farfán-Cerda1
1 Unidad de Cariología. Departamento de Rehabilitación Buco-Máxilofacial, Universidad de Talca. Talca, Chile
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Información del artículo
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Resumen
Introducción

Se considera que Streptococcus mutans (S. mutans) y Lactobacillus spp. se asocian con la caries. Otras especies del biofilm oral, como Streptococcus sanguinis (S. sanguinis) han sido sindicadas como protectoras, pero principalmente en niños. Existe escasa evidencia sobre el nivel de estas bacterias en adultos mayores.

Objetivo

Determinar si existen diferencias en los recuentos microbianos de tres especies relacionadas con la caries en pacientes adultos y adultos mayores.

Materiales y Métodos

Una muestra de pacientes por conveniencia compuesta de 63 pacientes (18 a 79 años) proporcionó saliva estimulada con la que se sembraron placas de agar MSB, MM10 SB y Agar Rogosa para el cultivo de S. mutans, S. sanguinis y Lactobacillus spp., respectivamente. Los recuentos bacterianos fueron expresados como UFC/mL.

Resultados

Los recuentos de S. mutans y Lactobacillus spp. no mostraron variaciones relacionadas con la edad (p>0.05). Los adultos mostraron mayores recuentos de S. sanguinis que los adultos mayores, 3.7 x 105±3.8 × 105UFC/mL y 5.9 × 104±9.4 × 104UFC/mL, respectivamente (p<0.05).

Conclusiones

La edad no parece afectar los niveles de especies tradicionalmente consideradas como cariogénicas. Estos resultados sugieren que la edad puede relacionarse con los patrones de colonización de S. sanguinis en el biofilm oral.

Palabras clave:
S. mutans
S. sanguinis
Lactobacillus spp.
adultos mayores
caries
biofilm oral
Abstract
Introduction

Streptococcus mutans (S. mutans) and Lactobacillus spp. have been traditionally associated with caries, regardless of the subject's age. Other oral biofilm species have been linked as caries protective, including Streptococcus sanguinis (S. sanguinis), but mainly in children. Scarce evidence exists on the levels of these bacteria in older adults.

Aim

To determine whether there are differences in the microbial counts of three caries-associated bacterial species in adults and older adults.

Methodology

A convenience sample of sixty three patients, aged 18 to 79 years, participated in the study. Stimulated saliva samples were obtained and in MSB, MM10 and Rogosa agar plates for the culture of S. mutans, S. sanguinis and Lactobacillus spp., respectively. Bacterial counts were obtained by microscopic observation (10x) of the colonies and expressed in CFU/mL.

Results

Bacterial counts of S. mutans and Lactobacillus spp. did not reveal age-related differences (p>0.05). Adults showed higher S. sanguinis counts than older adults with 3.7 x 105±3.8 × 105CFU/mL and 5.9 × 104±9.4 × 104CFU/mL, respectively (p<0.05).

Conclusions

Age does not seem to affect the levels of bacterial species traditionally associated with caries. The results suggest that age may be related to colonization patterns of S. sanguinis in the oral biofilm.

Key words:
S. mutans
S. sanguinis
Lactobacillus spp.
older adults
caries
oral biofilm
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Referencias Bibliográficas
[1.]
P. Petersen, D. Bourgeois, H. Ogawa, S. Estupinan-Day, C. Ndiaye.
The global burden of oral diseases and risks to oral health.
Bull World Health Organ, 83 (2005), pp. 661-669
http://dx.doi.org//S0042-96862005000900011 | Medline
[2.]
L.B. Shrestha.
Population aging in developing countries.
Health Aff (Millwood), 19 (2000), pp. 204-212
[3.]
S.S. Socransky, S.D. Manganiello.
The oral microbiota of man from birth to senility.
J Periodontol, 42 (1971), pp. 485-496
http://dx.doi.org/10.1902/jop.1971.42.8.485 | Medline
[4.]
P.D. Marsh.
Microbiology of dental plaque biofilms and their role in oral health and caries.
Dent Clin North Am, 54 (2010), pp. 441-454
http://dx.doi.org/10.1016/j.cden.2010.03.002 | Medline
[5.]
N. Takahashi, B. Nyvad.
The role of bacteria in the caries process: Ecological perspectives.
J Dent Res, 90 (2011), pp. 294-303
http://dx.doi.org/10.1177/0022034510379602 | Medline
[6.]
G.H. Bowden, J. Ekstrand, B. McNaughton, S.J. Challacombe.
Association of selected bacteria with the lesions of root surface caries.
Oral Microbiol Immunol, 5 (1990), pp. 346-351
Medline
[7.]
P. Schüpbach, V. Osterwalder, B. Guggenheim.
Human root caries: Microbiota of a limited number of root caries lesions.
Caries Res, 30 (1996), pp. 52-64
Medline
[8.]
J. van Houte.
Role of micro-organisms in caries etiology.
J Dent Res, 73 (1994), pp. 672-681
Medline
[9.]
W.J. Loesche.
Role of Streptococcus mutans in human dental decay.
Microbiol Rev, 50 (1986), pp. 353-380
Medline
[10.]
J.M. Tanzer, J. Livingston, A.M. Thompson.
The microbiology of primary dental caries in humans.
J Dent Educ, 65 (2001), pp. 1028-1037
Medline
[11.]
R.S. Percival, S.J. Challacombe, P.D. Marsh.
Age-related microbiological changes in the salivary and plaque microflora of healthy adults.
J Med Microbiol, 35 (1991), pp. 5-11
http://dx.doi.org/10.1099/00222615-35-1-5 | Medline
[12.]
R.A. Giacaman, E. Araneda, C. Padilla.
Association between biofilm-forming isolates of mutans streptococci and caries experience in adults.
Arch Oral Biol, 55 (2010), pp. 550-554
http://dx.doi.org/10.1016/j.archoralbio.2010.05.010 | Medline
[13.]
M.J. Schaeken, T.J. Creugers, J.S. Van der Hoeven.
Relationship between dental plaque indices and bacteria in dental plaque and those in saliva.
J Dent Res, 66 (1987), pp. 1499-1502
Medline
[14.]
J. Kreth, J. Merritt, W. Shi, F. Qi.
Competition and coexistence between Streptococcus mutans and Streptococcus sanguinis in the dental biofilm.
J Bacteriol, 187 (2005), pp. 7193-7203
http://dx.doi.org/10.1128/JB.187.21.7193-7203.2005 | Medline
[15.]
J. Kreth, Y. Zhang, M.C. Herzberg.
Streptococcal antagonism in oral biofilms: Streptococcus sanguinis and Streptococcus gordonii interference with Streptococcus mutans.
J Bacteriol, 190 (2008), pp. 4632-4640
http://dx.doi.org/10.1128/JB.00276-08 | Medline
[16.]
W.J. Loesche, A. Walenga, P. Loos.
Recovery of Streptococcus mutans and Streptococcus sanguis from a dental explorer after clinical examination of single human teeth.
Arch Oral Biol, 18 (1973), pp. 571-575
Medline
[17.]
Y. Ge, P.W. Caufield, G.S. Fisch, Y. Li.
Streptococcus mutans and Streptococcus sanguinis colonization correlated with caries experience in children.
Caries Res, 42 (2008), pp. 444-448
http://dx.doi.org/10.1159/000159608 | Medline
[18.]
P.W. Caufield, A.P. Dasanayake, Y. Li, Y. Pan, J. Hsu, J.M. Hardin.
Natural history of Streptococcus sanguinis in the oral cavity of infants: Evidence for a discrete window of infectivity.
Infect Immun, 68 (2000), pp. 4018-4023
Medline
[19.]
J. van Houte, J. Lopman, R. Kent.
The final pH of bacteria comprising the predominant flora on sound and carious human root and enamel surfaces.
J Dent Res, 75 (1996), pp. 1008-1014
Medline
[20.]
D. Preza, I. Olsen, T. Willumsen, B. Grinde, B.J. Paster.
Diversity and site-specificity of the oral microflora in the elderly.
Eur J Clin Microbiol Infect Dis, 28 (2009), pp. 1033-1040
http://dx.doi.org/10.1007/s10096-009-0743-3 | Medline
[21.]
U. Heintze, G. Frostell, F. Lindgärde, E. Trell.
Secretion rate and buffer effect of resting and stimulated whole saliva in relation to general health.
Swed Dent J, 10 (1986), pp. 213-219
Medline
[22.]
O.G. Gold, H.V. Jordan, J. Van Houte.
A selective medium for Streptococcus mutans.
Arch Oral Biol, 18 (1973), pp. 1357-1364
Medline
[23.]
S.A. Syed, W.J. Loesche.
Survival of human dental plaque flora in various transport media.
Appl Microbiol, 24 (1972), pp. 638-644
Medline
[24.]
M. Rogosa, J.A. Mitchell, R.F. Wiseman.
A selective medium for the isolation and enumeration of oral lactobacilli.
J Dent Res, 30 (1951), pp. 682-689
Medline
[25.]
C.G. Emilson.
Prevalence of Streptococcus mutans with different colonial morphologies in human plaque and saliva.
Scand J Dent Res, 91 (1983), pp. 26-32
Medline
[26.]
W. Little, D. Korts, L. Thomson, W. Bowen.
Comparative recovery of Streptococcus mutans on ten isolation media.
J Clin Microbiol, 5 (1977), pp. 578-583
Medline
[27.]
S.A. Syed, W.J. Loesche.
Efficiency of various growth media in recovering oral bacterial flora from human dental plaque.
Appl Microbiol, 26 (1973), pp. 459-465
Medline
[28.]
C. Llena-Puy.
The role of saliva in maintaining oral health and as an aid to diagnosis.
Med Oral Patol Oral Cir Bucal, 11 (2006), pp. E449-E455
Medline
[29.]
S. Contardo, N. Díaz, O. Lobos, C. Padilla, R.A. Giacaman.
Oral colonization by Streptococcus mutans and its association with the severity of periodontal disease in adults.
Rev Clin Periodoncia Implantol Rehabil Oral, 4 (2011), pp. 9-13
[30.]
J. van Houte.
Role of micro-organisms in caries etiology.
J Dent Res, 73 (1994), pp. 672-681
Medline
[31.]
D. Preza, I. Olsen, J.A. Aas, T. Willumsen, B. Grinde, B.J. Paster.
Bacterial profiles of root caries in elderly patients.
J Clin Microbiol, 46 (2008), pp. 2015-2021
http://dx.doi.org/10.1128/JCM.02411-07 | Medline
[32.]
MINSAL. Diagnostic of the Oral Health Situation. Available at: http://www.redsalud.gov.cl/archivos/salud_bucal/perfilepidemiologico.pdf. Accessed: Feb 2, 2012; 2007.
[33.]
J. Carlsson, H. Grahnen, G. Jonsson, S. Wikner.
Establishment of Streptococcus sanguis in the mouths of infants.
Arch Oral Biol, 15 (1970), pp. 1143-1148
Medline
[34.]
J.S. van der Hoeven, P.J. Camp.
Mixed continuous cultures of Streptococcus mutans with Streptococcus sanguis or with Streptococcus oralis as a model to study the ecological effects of the lactoperoxidase system.
Caries Res, 27 (1993), pp. 26-30
Medline
[35.]
J.D. De Stoppelaar, J. Van Houte, O. Backer Dirks.
The relationship between extracellular polysaccharide-producing streptococci and smooth surface caries in 13-year-old children.
Caries Res, 3 (1969), pp. 190-199
Medline
[36.]
W.J. Loesche, R.N. Hockett, S.A. Syed.
The predominant cultivable flora of tooth surface plaque removed from institutionalized subjects.
Arch Oral Biol, 17 (1972), pp. 1311-1325
Medline
[37.]
Y.P. Pan, Y. Li, P.W. Caufield.
Phenotypic and genotypic diversity of Streptococcus sanguis in infants.
Oral Microbiol Immunol Denmark, (2001), pp. 235-242
[38.]
S. Gizani, W. Papaioannou, A.D. Haffajee, K. Kavvadia, M. Quirynen, L. Papagiannoulis.
Distribution of selected cariogenic bacteria in five different intra-oral habitats in young children.
Int J Paediatr Dent England, (2009), pp. 193-200
[39.]
H. Tong, W. Chen, J. Merritt, F. Qi, W. Shi, X. Dong.
Streptococcus oligofermentans inhibits Streptococcus mutans through conversion of lactic acid into inhibitory H2O2: A possible counteroffensive strategy for interspecies competition.
Mol Microbiol England, (2007), pp. 872-880
[40.]
W.J. Loesche.
Chemotherapy of dental plaque infections.
Oral Sci Rev, 9 (1976), pp. 65-107
Medline
[41.]
S.M. Alaki, W.J. Loesche, M.A. da Fonesca, R.J. Feigal, K. Welch.
Preventing the transfer of Streptococcus mutans from primary molars to permanent first molars using chlorhexidine.
Pediatr Dent, 24 (2002), pp. 103-108
Medline
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