P- 59 ROLE OF MARESINA-1 ON THE KIDNEY AND LIVER MORPHOLOGY ON A MURINE MODEL OF TYPE I DIABETES MELLITUS INDUCED WITH STREPTOZOTOCIN

Morales, Pamela Carolina; Zúñiga, Jessica Pamela; Norambuena, Ramón; González, Daniel

doi:10.1016/j.aohep.2023.101246

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THE KIDNEY AND LIVER MORPHOLOGY ON A MURINE MODEL OF TYPE I DIABETES MELLITUS INDUCED WITH STREPTOZOTOCIN" "tieneTextoCompleto" => 0 "autores" => array:1 [ 0 => array:3 [ "autoresLista" => "Pamela Carolina Morales, Jessica Pamela Zúñiga, Ramón Norambuena, Daniel González" "autores" => array:4 [ 0 => array:3 [ "nombre" => "Pamela Carolina" "apellidos" => "Morales" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "1" "identificador" => "aff0001" ] ] ] 1 => array:3 [ "nombre" => "Jessica Pamela" "apellidos" => "Zúñiga" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "2" "identificador" => "aff0002" ] ] ] 2 => array:3 [ "nombre" => "Ramón" "apellidos" => "Norambuena" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "2" "identificador" => "aff0002" ] ] ] 3 => array:3 [ "nombre" => "Daniel" "apellidos" => "González" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "2" "identificador" => "aff0002" ] ] ] ] "afiliaciones" => array:2 [ 0 => array:3 [ "entidad" => "Basical Biomedical Science Department, Department of Health Sciences, University of Talca, Talca, Chile" "etiqueta" => "1" "identificador" => "aff0001" ] 1 => array:3 [ "entidad" => "Department of Natural Resources and Veterinary Medicine, School of Veterinary Medicine. Santo Tomas University, Talca, Chile" "etiqueta" => "2" "identificador" => "aff0002" ] ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "tieneResumen" => true "resumen" => array:1 [ "en" => array:2 [ "resumen" => "Introduction and ObjectivesDiabetes mellitus (DM) is a chronic metabolic disease characterized by elevated blood glucose levels. DM1 manifests itself through hyperglycemia, the main factor inducing numerous life-threatening complications and comorbidities. DM has high morbidity and mortality associated with cardiovascular diseases, nephropathy and dyslipidemias, which are correlated with the deterioration of liver function. Maresin-1 (MaR1), a specialized pro-resolving lipid mediators has recently been described. MaR1 has a positive role in various inflammation-related pathologies particularly, it has favorable effect on chronic liver disease. This study aims to evaluate the effect of MaR1 administration on liver and kidney tissues in a murine model of DM1. Our hypothesis is that treatment produces an improvement in liver and kidney morphology. Materials and Methodsmale C57BL/6 mice where induced DM1 by Spreptozotocin injection (50mg/Kg) and MaR1 (4ng/g) diary for 4 weeks. Liver and Kidneys were processed for H&E and Masson´s Trichrome stain and morphological analyzed. An immunohistochemical study was also carried out with the use of antibodies to fibronectin and -SMA. The data was evaluated by Image J and Prism 9 software's. ResultsBoth in liver and kidney, the MaR1 animal group recovers the cito-architecture and decreased the score of fibrosis in comparison with DM group, resembling the control group. In liver tissue a decrease of lipids deposit was observed in the MaR1, concomitant with the architecture improvement. ConclusionsMar1 at 4 ng/g generates a positive response in the liver and kidney morphology, depleting the tissue damage observed in a DM1 murine model. Deeper analysis should carry on for to enlarge this results and assay Mar1 as a potential therapeutic activity against one of the most prevalent diseases in the world, the DM." "secciones" => array:4 [ 0 => array:2 [ "identificador" => "abss0001" "titulo" => "Introduction and Objectives" ] 1 => array:2 [ "identificador" => "abss0002" "titulo" => "Materials and Methods" ] 2 => array:2 [ "identificador" => "abss0003" "titulo" => "Results" ] 3 => array:2 [ "identificador" => "abss0004" "titulo" => "Conclusions" ] ] ] ] ] "idiomaDefecto" => "en" "url" => "/16652681/00000029000000S1/v2_202405310451/S1665268123003496/v2_202405310451/en/main.assets" "Apartado" => null "PDF" => "https://static.elsevier.es/multimedia/16652681/00000029000000S1/v2_202405310451/S1665268123003496/v2_202405310451/en/main.pdf?idApp=UINPBA00004N&text.app=https://www.elsevier.es/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1665268123003496?idApp=UINPBA00004N"]

ISSN: 1665-2681

Annals of Hepatology (AoH) is an international, open access journal published bi-monthly with funds from the Fundación Clínica Médica Sur. It is the official journal of the Mexican Association of Hepatology (AMH), the Latin American Association for the Study of the Liver (ALEH), the Canadian Association for the Study of the Liver (CASL) and the Czech Society of Hepatology (CSH). AoH publishes editorials, opinions, concise reviews, original articles, brief reports, letters to the editor, news from affiliated associations, clinical practice guidelines and summaries of congresses in the field of Hepatology.

Topics covered by AoH include alcoholic liver disease, autoimmune hepatitis, biliary diseases, drug-induced liver injury, genetic liver diseases, NAFLD/NASH and viral hepatitis (HAV, HBV, HCV, HDV, HEV). Our journal seeks to publish articles on basic clinical care and translational research focused on preventing rather than treating the complications of end-stage liver disease.

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Diabetes mellitus (DM) is a chronic metabolic disease characterized by elevated blood glucose levels. DM1 manifests itself through hyperglycemia, the main factor inducing numerous life-threatening complications and comorbidities. DM has high morbidity and mortality associated with cardiovascular diseases, nephropathy and dyslipidemias, which are correlated with the deterioration of liver function. Maresin-1 (MaR1), a specialized pro-resolving lipid mediators has recently been described. MaR1 has a positive role in various inflammation-related pathologies particularly, it has favorable effect on chronic liver disease. This study aims to evaluate the effect of MaR1 administration on liver and kidney tissues in a murine model of DM1. Our hypothesis is that treatment produces an improvement in liver and kidney morphology.

Materials and Methods

male C57BL/6 mice where induced DM1 by Spreptozotocin injection (50mg/Kg) and MaR1 (4ng/g) diary for 4 weeks. Liver and Kidneys were processed for H&E and Masson´s Trichrome stain and morphological analyzed. An immunohistochemical study was also carried out with the use of antibodies to fibronectin and -SMA. The data was evaluated by Image J and Prism 9 software's.

Results

Both in liver and kidney, the MaR1 animal group recovers the cito-architecture and decreased the score of fibrosis in comparison with DM group, resembling the control group. In liver tissue a decrease of lipids deposit was observed in the MaR1, concomitant with the architecture improvement.

Conclusions

Mar1 at 4 ng/g generates a positive response in the liver and kidney morphology, depleting the tissue damage observed in a DM1 murine model. Deeper analysis should carry on for to enlarge this results and assay Mar1 as a potential therapeutic activity against one of the most prevalent diseases in the world, the DM.

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