Antioxidant effect of Moringa oleifera leaves in hemoglobin oxidation compare with vitamin C

Harun, Haerani; Daud, Anwar; Hadju, Veni; Arief, Credo Pratama Putra; Talebe, Tamrin; Rahma; Wahyuni, Rosa Dwi; Sumarni; Miranti; Amri, Imtihanah; Faris, Abdul; Mallongi, Anwar

doi:10.1016/j.enfcli.2019.10.033

ISSN: 1130-8621

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Vol. 30. Issue S4.

First International Conference on Nutrition and Public Health (ICNPH-2019)

Pages 18-21 (June 2020)

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Vol. 30. Issue S4.

First International Conference on Nutrition and Public Health (ICNPH-2019)

Pages 18-21 (June 2020)

DOI: 10.1016/j.enfcli.2019.10.033

Antioxidant effect of Moringa oleifera leaves in hemoglobin oxidation compare with vitamin C

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Haerani Haruna,

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queen196um@gmail.com

Corresponding author.

, Anwar Daudb, Veni Hadjuc, Credo Pratama Putra Ariefd, Tamrin Talebee, Rahmaa, Rosa Dwi Wahyunia, Sumarnia, Mirantia, Imtihanah Amria, Abdul Farisa, Anwar Mallongib

a Student Doctoral Degree of Public Health Faculty, Hasanuddin University, Indonesia

b Department of Enviromental Health, Pablic Health Faculty, Hasanuddin University, Indonesia

c Department of Nutrition, Public Health Faculty, Hasanuddin University, Indonesia

d Faculty of Medicine, Tadulako University, Indonesia

e Faculty of Ushuluddin, Islamic Institute of Datokarama, Indonesia

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Tables (2)

Table 1. Metbemoglobin levels based on ontioxidant activity of Vitamin C, MO leaves extract and saline.

Table 2. Post hoc results of methemoglobin levels in each treatment.

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Abstract

Objective

The aim of this study is to investigated the effect of MO leaves extract as an antioxidant in inhibiting methemoglobin (MetHb) formation compared with Vitamin C as positive control.

Methods

This study was an experimental study in vitro comparing the antioxidant effects of MO leaves extract with vitamin C as positive control and saline as a negative control. Hemoglobin was oxidized with potassium ferrocyanide then the antioxidant effect of MO leaf extract was determined by measuring methemoglobin levels.

Results

This study showed that MO leaves extract have antioxidant effects compared to saline, MO leaves extract 1600ppm showed no significant differences with Vitamin C 1600ppm (p=0.067). MO leaves extract 3200ppm showed no significant difference with all Vitamin C levels (p>0.05). This shows that MO leaves extract has antioxidant effects like Vitamin C.

Conclusion

M. oleifera leaves extract has antioxidant effects on hemoglobin oxidation induced by potassium ferricyanida.

Keywords:

Moringa oleifera

Antioxidants

Methemoglobin

Hemoglobin oxidation

Vitamin C

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