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Original article
Effects of sevoflurane on ventilator induced lung injury in a healthy lung experimental model
Efectos de sevoflurano en la lesión pulmonar inducida por la ventilación mecánica en un modelo experimental de pulmón sano
A. Romero
Corresponding author
antonromero@hotmail.com

Corresponding author.
, A. Moreno, J. García, C. Sánchez, M. Santos, J. García
Departamento de Anestesiología, Reanimación y Cuidados Críticos, Hospital Universitario Puerta de Hierro-Majadahonda, Majadahonda, Madrid, Spain
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    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Most patients undergoing general anesthesia and a large percentage of patients admitted to intensive care units receive mechanical ventilation in order to alleviate the work of breathing while lung function is restored&#46; Incorrect use of mechanical ventilation can damage the lungs or aggravate an existing lung injury&#44; and give rise to ventilator-induced lung injury &#40;VILI&#41;<a class="elsevierStyleCrossRefs" href="#bib0135"><span class="elsevierStyleSup">1&#44;2</span></a> caused by an amplification and generalization of the systemic inflammatory response &#40;biotrauma&#41;&#46; During this inflammatory response&#44; blood and tissue levels of proinflammatory cytokines such as the tumor necrosis factor &#40;TNF-&#945;&#41;&#44; Interleukin &#40;IL&#41; 1&#946;&#44; IL-6 and la IL-8 are increased&#46;<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">3</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">VILI is mainly associated with phenomena such as the use of insufficient positive end-expiratory pressure &#40;PEEP&#41; to prevent cyclic alveolar collapse-reopening &#40;atelectrauma&#41;&#44; which increases perialveolar leukocyte infiltration<a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">4</span></a>&#59; the delivery of high alveolar pressure &#40;barotrauma&#41;&#44; which causes alveolar and perivascular edema<a class="elsevierStyleCrossRef" href="#bib0155"><span class="elsevierStyleSup">5</span></a>&#59; and the use of high respiratory frequency&#44; due to stress cycles&#46;<a class="elsevierStyleCrossRef" href="#bib0160"><span class="elsevierStyleSup">6</span></a> The damaging effect of high tidal volumes &#40;volutrauma&#41;&#44; which can induce overdistension of alveoli&#44; has also been suggested as a cause of VILI&#46;<a class="elsevierStyleCrossRefs" href="#bib0165"><span class="elsevierStyleSup">7&#44;8</span></a></p><p id="par0015" class="elsevierStylePara elsevierViewall">A classic experimental VILI induction model involves the delivery of cyclic inflation pressures &#40;&#62;30<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O&#41; at different time intervals&#46;<a class="elsevierStyleCrossRef" href="#bib0165"><span class="elsevierStyleSup">7</span></a></p><p id="par0020" class="elsevierStylePara elsevierViewall">Volatile anesthetics have been shown to have an anti-inflammatory action&#46;<a class="elsevierStyleCrossRef" href="#bib0175"><span class="elsevierStyleSup">9</span></a> Therefore&#44; volatile anesthetics attenuate ischemia&#8211;reperfusion injury in the heart&#44;<a class="elsevierStyleCrossRef" href="#bib0180"><span class="elsevierStyleSup">10</span></a> the kidney&#44;<a class="elsevierStyleCrossRef" href="#bib0185"><span class="elsevierStyleSup">11</span></a> the liver<a class="elsevierStyleCrossRef" href="#bib0190"><span class="elsevierStyleSup">12</span></a> and the lungs&#44;<a class="elsevierStyleCrossRefs" href="#bib0195"><span class="elsevierStyleSup">13&#44;14</span></a> and decrease the inflammatory response in <span class="elsevierStyleItalic">in vivo</span><a class="elsevierStyleCrossRefs" href="#bib0175"><span class="elsevierStyleSup">9&#44;15&#8211;17</span></a> and <span class="elsevierStyleItalic">in vitro</span><a class="elsevierStyleCrossRef" href="#bib0215"><span class="elsevierStyleSup">17</span></a> sepsis-induced pulmonary injury models&#46; Furthermore&#44; recent studies have shown that sevoflurane could act as a pre- and postconditioning agent in endotoxin-induced lung injury models&#46;<a class="elsevierStyleCrossRef" href="#bib0220"><span class="elsevierStyleSup">18</span></a> Nevertheless&#44; no studies have explored the possible protective effect of sevoflurane against VILI in previously healthy lungs&#46;</p><p id="par0025" class="elsevierStylePara elsevierViewall">The aim of this study is to evaluate the effect of sevoflurane on VILI&#44; analyzing histopathological damage&#44; the degree of pulmonary edema&#44; and level of inflammatory cytokines in rats&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Materials and methods</span><p id="par0030" class="elsevierStylePara elsevierViewall">We studied 20 Wistar rats with a mean weight of 215<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>35<span class="elsevierStyleHsp" style=""></span>g&#46; The animals were kept in groups of 6 in U-TEMP polyetherimide cages&#46; They were given free access to food and water&#44; with 12<span class="elsevierStyleHsp" style=""></span>h of light and 12<span class="elsevierStyleHsp" style=""></span>h of darkness&#44; a temperature of 20<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>2<span class="elsevierStyleHsp" style=""></span>&#176;C and a relative humidity of 50&#8211;70&#37;&#46; The animals were kept in these conditions for at least 1 week to allow them to acclimatize before starting the experiments&#46; The animals were handled in accordance with European and Spanish laws governing the protection of experimental animals &#40;2010&#47;63&#47;EU and RD 53&#47;2013&#41;&#44; and the study was approved by the institutional Animal Experimentation Ethics Committee&#46;</p><p id="par0035" class="elsevierStylePara elsevierViewall">The animals were anesthetized with an intraperitoneal injection of ketamine &#40;75<span class="elsevierStyleHsp" style=""></span>mg&#47;kg&#41; and diazepam &#40;5<span class="elsevierStyleHsp" style=""></span>mg&#47;kg&#41;&#46; When the target level of hypnosis had been reached&#44; the animal was intubated using a 16<span class="elsevierStyleHsp" style=""></span>G polyethylene catheter &#40;Abbott Ireland&#44; Sligo&#44; Republic of Ireland&#41;&#44; using the otoscope method<a class="elsevierStyleCrossRef" href="#bib0225"><span class="elsevierStyleSup">19</span></a> to connect the catheter to the semi-closed anesthesia breathing system &#40;Julian&#44; Dr&#228;ger&#44; L&#252;beck&#44; Germany&#41;&#46;</p><p id="par0040" class="elsevierStylePara elsevierViewall">Following this&#44; pressure-controlled mechanical ventilation was started&#44; with a peak inspiratory pressure &#40;PIP&#41; of 12<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O&#44; PEEP of 3<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O&#44; an FiO<span class="elsevierStyleInf">2</span> of 0&#46;5&#44; and a breathing rate of 30<span class="elsevierStyleHsp" style=""></span>rpm&#46;</p><p id="par0045" class="elsevierStylePara elsevierViewall">The trachea was dissected by means of a medial incision in the neck&#46; The airway was tied off using suture thread&#44; a line was placed in the left carotid artery &#40;24<span class="elsevierStyleHsp" style=""></span>G polyethylene catheter&#44; Abbott Ireland&#41; to monitor heart rate &#40;HR&#41; and mean arterial pressure with a calibrated pressure transducer&#44; and arterial blood samples were taken to determine pH&#44; PaO<span class="elsevierStyleInf">2</span> and PaCO<span class="elsevierStyleInf">2</span>&#46;</p><p id="par0050" class="elsevierStylePara elsevierViewall">A 24<span class="elsevierStyleHsp" style=""></span>G polyethylene catheter &#40;Abbott Ireland&#41; was placed in the tail vein to administer 10<span class="elsevierStyleHsp" style=""></span>mL&#47;kg&#47;h 0&#46;9&#37; physiological saline solution&#44; and a temperature probe was placed in the rectum&#46; An air heater set to 45<span class="elsevierStyleHsp" style=""></span>&#176;C was used to maintain room temperature within physiological limits &#40;37&#8211;38<span class="elsevierStyleHsp" style=""></span>&#176;C&#41;&#46;</p><p id="par0055" class="elsevierStylePara elsevierViewall">Following this&#44; the animals were randomized to one of two study groups&#58; CONTROL &#40;n<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>10&#41;&#44; which did not receive sevoflurane&#44; and SEV &#40;n<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>10&#41;&#44; which were ventilated with 3&#37; sevoflurane vaporized in 1<span class="elsevierStyleHsp" style=""></span>l&#47;min of oxygen&#46;</p><p id="par0060" class="elsevierStylePara elsevierViewall">After 30<span class="elsevierStyleHsp" style=""></span>min &#40;BASELINE time&#41;&#44; VILI was induced by increasing PIP to 35<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O with a driving pressure of 35<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O for 20<span class="elsevierStyleHsp" style=""></span>min &#40;INJURY time&#41;&#46; Following this&#44; ventilation continued for 30<span class="elsevierStyleHsp" style=""></span>min at the initial setting of 12<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O peak pressure and 3<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O PEEP &#40;POSTINJURY time&#41; in order to allow time for proinflammatory cytokine levels in lung tissue to increase&#46; During this process&#44; the SEV group continued to receive 3&#37; sevoflurane&#46; At the end of each experiment&#44; the animals were sacrificed with 50<span class="elsevierStyleHsp" style=""></span>meq&#47;kg of intravenous potassium chloride and the lungs were removed to study cytokine levels&#44; the wet&#47;dry weight ratio&#44; and to perform histopathological studies&#46;</p><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Hemodynamic and blood gas parameters</span><p id="par0065" class="elsevierStylePara elsevierViewall">HR&#44; mean arterial pressure and arterial blood gases &#40;pH&#44; PaO<span class="elsevierStyleInf">2</span> and PaCO<span class="elsevierStyleInf">2</span>&#41; were measured immediately prior to lung injury induction &#40;baseline&#41;&#44; 20<span class="elsevierStyleHsp" style=""></span>min after start of VILI&#44; and at the end of the study &#40;30<span class="elsevierStyleHsp" style=""></span>min POSTINJURY&#41;&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Determination of the wet&#47;dry lung weight ratio</span><p id="par0070" class="elsevierStylePara elsevierViewall">The wet&#47;dry weight ratio was calculated in the whole left lungs of all study animals&#46; Lungs were dehydrated in a thermostat-controlled chamber at 80<span class="elsevierStyleHsp" style=""></span>&#176;C for 72<span class="elsevierStyleHsp" style=""></span>h and the dry weight was measured&#46; The wet&#47;dry weight ratio was calculated to evaluate the percentage of pulmonary edema&#46;</p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Determination of TNF-&#945;&#44; IL-1&#946; and IL-6 in lung tissue</span><p id="par0075" class="elsevierStylePara elsevierViewall">The same lung segment &#40;lower right lobe&#41; was excised from all rats and stored at &#8722;80<span class="elsevierStyleHsp" style=""></span>&#176;C until needed for study&#46; TNF-&#945;&#44; IL-1&#946; and IL-6 in lung tissue was measured&#46; The segment was place in saline solution and centrifuged for 10<span class="elsevierStyleHsp" style=""></span>min at 10&#44;000<span class="elsevierStyleHsp" style=""></span>rpm&#44; at 4<span class="elsevierStyleHsp" style=""></span>&#176;C&#46; Cytokine levels were measured in the supernatant by means of an ELISA test&#46;</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">Histopathological analysis</span><p id="par0080" class="elsevierStylePara elsevierViewall">The upper right lobes of all rats was excised and fixed in 10&#37; formaldehyde&#46; They were then stained with hematoxylin and eosin and embedded in paraffin for study by a histopathologist blinded to the study&#46;</p><p id="par0085" class="elsevierStylePara elsevierViewall">A 4-point qualitative ordinal variable injury scale &#40;negative<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#44; minor<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>1&#44; moderate<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>2&#44; severe<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>3&#41; was used to evaluate each of the following characteristics&#46; The 4 main characteristics based on 15 individual items are&#58; atelectasis&#44; edema &#40;septal edema&#44; interstitial edema&#44; lymphangiectasia&#44; intra-alveolar exudate&#41;&#44; inflammation &#40;alveolar neutrophil infiltration&#44; interstitial neutrophil infiltration&#44; interstitial lymphocyte infiltration&#44; granulocyte adhesion&#41;&#44; and other &#40;alveolar hemorrhage&#44; hyperemia&#44; thrombocyte aggregation&#44; fibrin deposits&#44; hyaline membrane formation&#44; peeling of the bronchial and bronchiolar epithelium&#41;&#46; A summary of the results obtained for each parameter in all animals is given below&#46; The sum of all 15 parameters was then divided by the number of animals in each group to obtain the total injury score&#46;</p></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">Statistical analysis</span><p id="par0090" class="elsevierStylePara elsevierViewall">Statistical analysis of study data was performed on SPSS<span class="elsevierStyleSup">&#174;</span> v&#46; 15&#46;0 &#40;SPSS Inc&#46;&#44; Chicago&#44; IL&#44; USA&#41;&#46; Based on the findings of earlier studies conducted by our group&#44; we determined that each group should comprise 7 animals in order to detect a difference in HR of 50<span class="elsevierStyleHsp" style=""></span>bpm or over&#44; with a <span class="elsevierStyleItalic">p</span> value of 0&#46;05&#44; a statistical power of 95&#37; in a bilateral comparison&#44; and a loss to follow-up or 0&#37;&#46;</p><p id="par0095" class="elsevierStylePara elsevierViewall">All data were grouped and summarized as mean<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>standard deviation&#46; Hemodynamic and blood gas parameters were analyzed using Shapiro&#8211;Wilk normality testing&#44; followed by repeated measures analysis of variance &#40;ANOVA&#41; testing and a Bonferroni multiple comparison test&#46; For inter-group comparison of wet&#47;dry weight ratio&#44; TNF-&#945;&#44; IL-1&#946; and IL-6&#44; the unpaired <span class="elsevierStyleItalic">t</span>-test was used&#46; Statistical significance was set at <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#46;</p></span></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0100">Results</span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0105">Hemodynamic and blood gas parameters</span><p id="par0100" class="elsevierStylePara elsevierViewall">Arterial blood gas analysis showed respiratory alkalosis in both groups at VILI time vs baseline time &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46; Difference between CONTROLs and the SEV group were not statistically significant&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><p id="par0105" class="elsevierStylePara elsevierViewall">Mean arterial pressure in the CONTROL group was significantly higher at the 3 study time points vs the SEV group &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46; No statistically significant differences in HR were observed&#46;</p></span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0110">Determination of the wet&#47;dry lung weight ratio</span><p id="par0110" class="elsevierStylePara elsevierViewall">Wet&#47;dry lung weight ratio as an indicator of the percentage of pulmonary edema was significantly lower in the SEV group vs CONTROLs &#40;79&#46;49&#37;<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1&#46;78&#37; vs 84&#46;97&#37;<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1&#46;69&#37;&#59; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;0001&#41; &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>&#41;&#46;</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0115">Determination of TNF-&#945;&#44; IL-1&#946; and IL-6 in lung tissue</span><p id="par0115" class="elsevierStylePara elsevierViewall">TNF-&#945; and IL-6 levels in lung tissue were significantly lower in the SEV group vs CONTROLs &#40;144<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>25 vs 243<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>51<span class="elsevierStyleHsp" style=""></span>pg&#47;mL for IL-6 &#91;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;0001&#93; and 14<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>4 vs 23<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>4<span class="elsevierStyleHsp" style=""></span>pg&#47;mL for TNF-&#945; &#91;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;0001&#93;&#41; &#40;<a class="elsevierStyleCrossRefs" href="#fig0010">Figs&#46; 2 and 3</a>&#41;&#59; however&#44; no statistically significant differences in changes in IL-1&#946; levels in lung tissue were found between groups &#40;128<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>92 in CONTROL vs 115<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>69<span class="elsevierStyleHsp" style=""></span>pg&#47;mL in SEV&#41; &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;715&#41; &#40;<a class="elsevierStyleCrossRef" href="#fig0015">Fig&#46; 3</a>&#41;&#46;</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia><elsevierMultimedia ident="fig0015"></elsevierMultimedia></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0120">Histopathological analysis</span><p id="par0120" class="elsevierStylePara elsevierViewall">Histopathological findings were similar in the sevoflurane group &#40;<a class="elsevierStyleCrossRef" href="#fig0020">Fig&#46; 4</a>&#41; and the CONTROL group &#40;<a class="elsevierStyleCrossRef" href="#fig0025">Fig&#46; 5</a>&#41;&#44; and showed peribronchial inflammatory injury&#44; edema&#44; and centrilobular emphysema&#46; The extent of injury was based on the score obtained from the 4-point qualitative ordinal variable scale&#58; the CONTROL group had a mean score of 1&#46;2<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;33&#44; with the SEV group scoring 0&#46;8<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;36&#44; which describes a minor injury with no statistically significant difference between groups&#46;</p><elsevierMultimedia ident="fig0020"></elsevierMultimedia><elsevierMultimedia ident="fig0025"></elsevierMultimedia></span></span><span id="sec0065" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0125">Discussion</span><p id="par0125" class="elsevierStylePara elsevierViewall">Following induction of VILI with an inflation pressure of 35<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O for 20<span class="elsevierStyleHsp" style=""></span>min&#44; neither study group showed any clinically significant severe histopathological injuries or oxygen deficit on examination&#46; This indicates that neither the pressure nor the duration of delivery was sufficient to induce severe histopathological lung injury&#44; or that postinjury time was not sufficient for these changes to show up on histopathological analysis&#46; Nevertheless&#44; the wet&#47;dry lung weight ratio&#44; which quantifies mechanical stress markers &#40;pulmonary edema and IL-6 and TNF-&#945; levels&#41;&#44; shows significantly less pulmonary edema and inflammatory response in the SEV group vs CONTROLs&#46;</p><p id="par0130" class="elsevierStylePara elsevierViewall">Other experimental studies have shown that mechanical ventilation with a driving pressure of 14<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O does not induce histological changes in the lung&#44; while subjects receiving driving pressures of between 30 and 45<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O present significant perivascular and alveolar edema after 30<span class="elsevierStyleHsp" style=""></span>min of delivery&#46;<a class="elsevierStyleCrossRefs" href="#bib0135"><span class="elsevierStyleSup">1&#44;2&#44;20</span></a> In our study&#44; we observed similar histopathological injuries using a driving pressure of 35<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O for 20<span class="elsevierStyleHsp" style=""></span>min&#44; although in this case the injuries were not as severe as those reported in the foregoing studies&#46; This shows that the time of exposure to high pressure &#40;increased from 20 to 30<span class="elsevierStyleHsp" style=""></span>min&#41; and the inflation pressure within this high pressure range &#40;30&#44; 35 or 40<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O&#41; are both key factors in determining the severity of lung injury&#46;</p><p id="par0135" class="elsevierStylePara elsevierViewall">In our study&#44; the quantification of pulmonary edema by means of the wet&#47;dry lung weight ratio&#44; and the quantification and measurement of proinflammatory cytokine levels were shown to be more sensitive early markers of inflation pressure-induced lung injury than histopathological injury&#44; as this latter requires a more prolonged exposure to high pressures&#46;</p><p id="par0140" class="elsevierStylePara elsevierViewall">Reduction in the extent of pulmonary edema in the sevoflurane group coincides with the findings obtained from pulmonary ischemia-reperfusion injury<a class="elsevierStyleCrossRef" href="#bib0200"><span class="elsevierStyleSup">14</span></a> and in sepsis-induced pulmonary injury models&#46;<a class="elsevierStyleCrossRef" href="#bib0215"><span class="elsevierStyleSup">17</span></a><span class="elsevierStyleItalic">In vitro</span>&#44; sevoflurane stimulates the Na&#43;&#47;K&#43;-ATPase activity in injury-induced type <span class="elsevierStyleSmallCaps">ii</span> alveolar epithelial cells&#44; one of the functions of which is to regulate transport of ClNa and H<span class="elsevierStyleInf">2</span>O into the alveolar space&#46;<a class="elsevierStyleCrossRef" href="#bib0235"><span class="elsevierStyleSup">21</span></a> In <span class="elsevierStyleItalic">in vivo</span> experiments&#44; however&#44; there is evidence that sevoflurane does not affect water reabsorption and edema resolution&#44; but could contribute to edema formation&#46;<a class="elsevierStyleCrossRef" href="#bib0215"><span class="elsevierStyleSup">17</span></a></p><p id="par0145" class="elsevierStylePara elsevierViewall">The antiinflammatory effect of volatile anesthetics has been explained by their involvement in preconditioning&#46;<a class="elsevierStyleCrossRef" href="#bib0175"><span class="elsevierStyleSup">9</span></a> Pharmacological preconditioning is a protection mechanism that makes cells relatively resistant to the myocellular death resulting from ischemia-reperfusion injury&#46;<a class="elsevierStyleCrossRef" href="#bib0205"><span class="elsevierStyleSup">15</span></a> In the lung&#44; volatile anesthetic-induced preconditioning protects against injury caused by both ischemia-reperfusion<a class="elsevierStyleCrossRefs" href="#bib0195"><span class="elsevierStyleSup">13&#44;14</span></a> and sepsis&#46;<a class="elsevierStyleCrossRefs" href="#bib0175"><span class="elsevierStyleSup">9&#44;15&#8211;17</span></a> Both types of injury are characterized by activation of proinflammatory cytokines&#44; such as TNF-&#945;&#44; IL-1&#946; and IL-6&#46;</p><p id="par0150" class="elsevierStylePara elsevierViewall">No significant differences in proinflammatory cytokines concentrations in bronchoalveolar lavage were found during mechanical ventilation with sevoflurane or pentobarbital<a class="elsevierStyleCrossRef" href="#bib0240"><span class="elsevierStyleSup">22</span></a>&#59; however&#44; VILI-induced inflammatory response is characterized by activation of these proinflammatory cytokines&#46;<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">3</span></a> The pulmonary homogenate obtained in our study shows less proinflammatory cytokine secretion in all cytokine secreting alveolar cells &#40;TNF-&#945; and IL-6&#44; but not IL-1&#946;&#41; in animals ventilated with sevoflurane&#46; This confirms that the same anti-inflammatory and cell protective action of sevoflurane reported in other lung injury models is also found in healthy lungs&#44; where it protects alveoli against damage induce by mechanical ventilation with high driving pressure&#46;</p><p id="par0155" class="elsevierStylePara elsevierViewall">Many alveolar cells involved in cytokine secretion&#44; such as alveolar macrophages<a class="elsevierStyleCrossRef" href="#bib0240"><span class="elsevierStyleSup">22</span></a> and polymorphonuclear leukocytes&#44;<a class="elsevierStyleCrossRef" href="#bib0210"><span class="elsevierStyleSup">16</span></a> can be affected by volatile anesthetics&#46; Type <span class="elsevierStyleSmallCaps">ii</span> alveolar epithelial cells&#44; together with inflammatory alveolar cells&#44; form part of the intra-alveolar cytokine network&#44; secreting IL-6&#44;<a class="elsevierStyleCrossRef" href="#bib0245"><span class="elsevierStyleSup">23</span></a> TNF-&#945;&#44;<a class="elsevierStyleCrossRef" href="#bib0250"><span class="elsevierStyleSup">24</span></a> and other interleukins&#46; Giraud et al&#46;<a class="elsevierStyleCrossRef" href="#bib0255"><span class="elsevierStyleSup">25</span></a> showed <span class="elsevierStyleItalic">in vitro</span> that volatile anesthetics in general&#44; and halotane in particular&#44; reduce secretion of TNF-&#945; and IL-6 in type <span class="elsevierStyleSmallCaps">ii</span> epithelial cells&#46; Subsequent <span class="elsevierStyleItalic">in vitro</span> experiments have shown the pre- and postconditioning with sevoflurane reduces expression of inflammatory mediators in alveolar epithelial cells&#46;<a class="elsevierStyleCrossRef" href="#bib0220"><span class="elsevierStyleSup">18</span></a></p><p id="par0160" class="elsevierStylePara elsevierViewall">In contrast to our results&#44; isoflurane and sevoflurane were found to reduce plasma concentrations of IL-1&#946; in a sepsis-induced lung injury model&#46;<a class="elsevierStyleCrossRef" href="#bib0205"><span class="elsevierStyleSup">15</span></a> We believe that no significant intra-group differences in IL-1&#946; levels were found in our study because samples were taken too soon to enable measurement of IL peaks in lung tissue &#40;30<span class="elsevierStyleHsp" style=""></span>min POSTINJURY&#41;&#46; This time frame was chosen to prevent loss of the IL-6 and TNF-&#945; peaks&#44; which occur earlier&#44; because alveolar macrophages secrete IL-1&#946; 8<span class="elsevierStyleHsp" style=""></span>h following lipopolysaccharide stimulation&#46;<a class="elsevierStyleCrossRef" href="#bib0260"><span class="elsevierStyleSup">26</span></a></p><p id="par0165" class="elsevierStylePara elsevierViewall">This study is subject to the limitation typical of any experimental study in rats&#46; This means that the results cannot be directly extrapolated to clinical practice in humans&#44; and more experimental studies are needed to confirm these preliminary findings&#46; Ultimately&#44; clinical trials should be conducted in human subjects to confirm our results&#46; Furthermore&#44; the VILI model used in our study was subject to a short injury exposure time &#40;20<span class="elsevierStyleHsp" style=""></span>min&#41;&#46; This means that although lung injury occurred&#44; it had no significant clinical impact on gas exchange&#46; Studies using higher pressure settings should be conducted&#44; above all with longer exposure times&#44; to show whether sevoflurane continues to exert an anti-inflammatory and cell protection effect even when lung damage is more severe and the clinical impact greater&#46;</p><p id="par0170" class="elsevierStylePara elsevierViewall">In conclusion&#44; our study shows that sevoflurane attenuates VILI in health lungs in a subclinical rat model of VILI&#46; Our findings suggest that further experimental and clinical studies are needed before the real clinical benefit of the cytoprotective effects of this anesthetic can be confirmed&#46;</p></span><span id="sec0070" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0130">Funding</span><p id="par0175" class="elsevierStylePara elsevierViewall">This study has been fully funded by departmental research funds managed by the <span class="elsevierStyleGrantSponsor" id="gs1">Instituto de Investigaci&#243;n del Hospital Puerta de Hierro</span>&#46;</p></span><span id="sec0075" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0135">Conflict of interest</span><p id="par0180" class="elsevierStylePara elsevierViewall">The authors declare they have no conflict of interest&#46;</p></span></span>"
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              "titulo" => "Determination of the wet&#47;dry lung weight ratio"
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              "titulo" => "Determination of TNF-&#945;&#44; IL-1&#946; and IL-6 in lung tissue"
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              "titulo" => "Determination of TNF-&#945;&#44; IL-1&#946; and IL-6 in lung tissue"
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              "titulo" => "Histopathological analysis"
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    "fechaRecibido" => "2015-01-10"
    "fechaAceptado" => "2015-04-13"
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          "clase" => "keyword"
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            0 => "Anesthesia"
            1 => "Lung injury"
            2 => "Mechanical ventilation"
            3 => "Inflammation"
            4 => "Sevoflurane"
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          "palabras" => array:5 [
            0 => "Anestesia"
            1 => "Da&#241;o pulmonar"
            2 => "Ventilaci&#243;n mec&#225;nica"
            3 => "Inflamaci&#243;n"
            4 => "Sevoflurano"
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        "titulo" => "Abstract"
        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Introduction and objective</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Ventilator-induced lung injury &#40;VILI&#41; causes a systemic inflammatory response in tissues&#44; with an increase in IL-1&#44; IL-6 and TNF-&#945; in blood and tissues&#46; Cytoprotective effects of sevoflurane in different experimental models are well known&#44; and this protective effect can also be observed in VILI&#46; The objective of this study was to assess the effects of sevoflurane in VILI&#46;</p></span> <span id="abst0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Material and methods</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">A prospective&#44; randomized&#44; controlled study was designed&#46; Twenty female rats were studied&#46; The animals were mechanically ventilated&#44; without sevoflurane in the control group and sevoflurane 3&#37; in the treated group &#40;SEV group&#41;&#46; VILI was induced applying a maximal inspiratory pressure of 35<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O for 20<span class="elsevierStyleHsp" style=""></span>min without any positive end-expiratory pressure for 20<span class="elsevierStyleHsp" style=""></span>min &#40;INJURY time&#41;&#46; The animals were then ventilated 30<span class="elsevierStyleHsp" style=""></span>min with a maximal inspiratory pressure of 12<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O and 3<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O positive end-expiratory pressure &#40;time 30<span class="elsevierStyleHsp" style=""></span>min POST-INJURY&#41;&#44; at which time the animals were euthanized and pathological and biomarkers studies were performed&#46; Heart rate&#44; invasive blood pressure&#44; pH&#44; PaO<span class="elsevierStyleInf">2</span>&#44; and PaCO<span class="elsevierStyleInf">2</span> were recorded&#46; The lung wet-to-dry weight ratio was used as an index of lung edema&#46;</p></span> <span id="abst0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Results</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">No differences were found in the blood gas analysis parameters or heart rate between the 2 groups&#46; Blood pressure was statistically higher in the control group&#44; but still within the normal clinical range&#46; The percentage of pulmonary edema and concentrations of TNF-&#945; and IL-6 in lung tissue in the SEV group were lower than in the control group&#46;</p></span> <span id="abst0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Conclusions</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">Sevoflurane attenuates VILI in a previous healthy lung in an experimental subclinical model in rats&#46;</p></span>"
        "secciones" => array:4 [
          0 => array:2 [
            "identificador" => "abst0005"
            "titulo" => "Introduction and objective"
          ]
          1 => array:2 [
            "identificador" => "abst0010"
            "titulo" => "Material and methods"
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        "resumen" => "<span id="abst0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Introducci&#243;n y objetivo</span><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">El da&#241;o pulmonar inducido por la ventilaci&#243;n mec&#225;nica &#40;VILI&#41; provoca una respuesta inflamatoria sist&#233;mica&#44; con elevaci&#243;n en sangre y tejidos de IL-1&#44; IL-6 y TNF-&#945;&#46; Conocidos los efectos citoprotectores de sevoflurano en diferentes modelos experimentales&#44; este podr&#237;a tener un comportamiento similar ante el VILI&#46; El objetivo de este estudio es valorar el efecto de sevoflurano en el VILI&#46;</p></span> <span id="abst0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Material y m&#233;todos</span><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Estudio experimental&#44; prospectivo&#44; controlado y aleatorizado&#46; Se utilizaron 20 ratas hembra&#46; Los animales fueron ventilados mec&#225;nicamente sin sevoflurano en el grupo control y con sevoflurano al 3&#37; en el grupo tratado &#40;grupo SEV&#41;&#46; Se provoc&#243; el VILI mediante una presi&#243;n inspiratoria m&#225;xima de 35<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O sin presi&#243;n positiva al final de la expiraci&#243;n durante 20<span class="elsevierStyleHsp" style=""></span>min &#40;tiempo LESI&#211;N&#41;&#46; Despu&#233;s&#44; los animales fueron ventilados 30<span class="elsevierStyleHsp" style=""></span>min con una presi&#243;n inspiratoria m&#225;xima de 12<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O y 3<span class="elsevierStyleHsp" style=""></span>cmH<span class="elsevierStyleInf">2</span>O de presi&#243;n positiva al final de la expiraci&#243;n &#40;tiempo 30<span class="elsevierStyleHsp" style=""></span>min POSLESI&#211;N&#41;&#46; Se registraron la frecuencia cardiaca&#44; las presiones arteriales sist&#243;lica&#44; diast&#243;lica y media&#44; el pH&#44; la PaO<span class="elsevierStyleInf">2</span> y la PaCO<span class="elsevierStyleInf">2</span>&#46; Se analiz&#243; la ratio peso h&#250;medo&#47;seco del tejido pulmonar&#46;</p></span> <span id="abst0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Resultados</span><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">No existieron diferencias estad&#237;sticamente significativas en los par&#225;metros gasom&#233;tricos ni en la frecuencia cardiaca entre los 2 grupos en estudio&#46; La PAM fue significativamente mayor en el grupo control&#44; pero dentro de l&#237;mites cl&#237;nicos normales&#46; El porcentaje de edema pulmonar y las concentraciones de TNF-&#945; e IL-6 en tejido pulmonar en el grupo de animales ventilados con sevoflurano fueron menores que en el grupo control&#46;</p></span> <span id="abst0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Conclusiones</span><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">Sevoflurano aten&#250;a el VILI en pulm&#243;n previamente sano&#44; en un modelo experimental de VILI subcl&#237;nico en ratas&#46;</p></span>"
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            "titulo" => "Material y m&#233;todos"
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        "etiqueta" => "&#9734;"
        "nota" => "<p class="elsevierStyleNotepara" id="npar0015">Please cite this article as&#58; Romero A&#44; Moreno A&#44; Garc&#237;a J&#44; S&#225;nchez C&#44; Santos M&#44; Garc&#237;a J&#46; Efectos de sevoflurano en la lesi&#243;n pulmonar inducida por la ventilaci&#243;n mec&#225;nica en un modelo experimental de pulm&#243;n sano&#46; Rev Esp Anestesiol Reanim&#46; 2016&#59;63&#58;22&#8211;28&#46;</p>"
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          "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Wet&#47;dry lung weight ratio as a marker of the percentage of pulmonary edema following establishment of a mechanical ventilation-induced lung injury in rat models anesthetized with &#40;SEV&#41; or without &#40;CONTROL&#41; sevoflurane&#46; &#42;Statistically significant &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#41; with respect to the CONTROL group&#46;</p>"
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                  \t\t\t\t</td><td class="td" title="table-entry  " align="" valign="top">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="" valign="top">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Baseline&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>30<span class="elsevierStyleHsp" style=""></span>min POSTINJURY&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">81<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>19<a class="elsevierStyleCrossRef" href="#tblfn0010"><span class="elsevierStyleSup">b</span></a>&nbsp;\t\t\t\t\t\t\n
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          "en" => "<p id="spar0070" class="elsevierStyleSimplePara elsevierViewall">Physiological parameters at baseline&#44; VILI&#44; and the end of the experiment in rats treated with &#40;SEV&#41; and without &#40;CONTROL&#41; sevoflurane&#46;</p>"
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      "titulo" => "References"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Ventilator-induced lung injury&#58; lessons from experimental studies"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "D&#46; Dreyfuss"
                            1 => "G&#46; Saumon"
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                    0 => array:2 [
                      "doi" => "10.1164/ajrccm.157.1.9604014"
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                        "volumen" => "157"
                        "paginaInicial" => "294"
                        "paginaFinal" => "323"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9445314"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
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              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
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                      "titulo" => "Ventilator-induced lung injury"
                      "autores" => array:1 [
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                          "etal" => false
                          "autores" => array:3 [
                            0 => "J&#46;D&#46; Ricard"
                            1 => "D&#46; Dreyfuss"
                            2 => "G&#46; Saumon"
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                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
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                        "fecha" => "2002"
                        "volumen" => "8"
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                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12205401"
                            "web" => "Medline"
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                  "contribucion" => array:1 [
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                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "H&#46;D&#46; Held"
                            1 => "S&#46; Boettcher"
                            2 => "L&#46; Hamann"
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                    0 => array:2 [
                      "doi" => "10.1164/ajrccm.163.3.2003001"
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                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "2001"
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                  "contribucion" => array:1 [
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                          "autores" => array:6 [
                            0 => "R&#46;B&#46; Goodman"
                            1 => "R&#46;M&#46; Strieter"
                            2 => "D&#46;P&#46; Martin"
                            3 => "K&#46;P&#46; Steinberg"
                            4 => "J&#46;A&#46; Milberg"
                            5 => "R&#46;J&#46; Maunder"
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                      "titulo" => "Experimental pulmonary edema due to intermittent positive pressure ventilation with high inflation pressures&#46; Protection by positive end-expiratory pressure"
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                        0 => array:2 [
                          "etal" => false
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                            1 => "D&#46;F&#46; Tierney"
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                    0 => array:2 [
                      "doi" => "10.1164/arrd.1974.110.5.556"
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                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/4611290"
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                0 => array:2 [
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                      "titulo" => "Effects of decreased respiratory frequency on ventilator-induced lung injury"
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                          "etal" => true
                          "autores" => array:6 [
                            0 => "J&#46;R&#46; Hotchkiss Jr&#46;"
                            1 => "L&#46; Blanch"
                            2 => "G&#46; Murias"
                            3 => "A&#46;B&#46; Adams"
                            4 => "D&#46;A&#46; Olson"
                            5 => "O&#46;D&#46; Wangensteen"
                          ]
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                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1164/ajrccm.161.2.9811008"
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                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "2000"
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                        "paginaInicial" => "463"
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                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10673186"
                            "web" => "Medline"
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                      ]
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                ]
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              "etiqueta" => "7"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "High inflation pressure pulmonary edema&#46; Respective effects of high airway pressure&#44; high tidal volume&#44; and positive end-expiratory pressure"
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                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "D&#46; Dreyfuss"
                            1 => "P&#46; Soler"
                            2 => "G&#46; Basset"
                            3 => "G&#46; Saumon"
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                        ]
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                    ]
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                    0 => array:2 [
                      "doi" => "10.1164/ajrccm/137.5.1159"
                      "Revista" => array:6 [
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                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/3057957"
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                      "titulo" => "The concept of &#8220;baby lung&#8221;"
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                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "L&#46; Gattinoni"
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                            5 => "R&#46;C&#46; Thompson"
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                        "paginaInicial" => "61"
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Article information
ISSN: 23411929
Original language: English
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