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Vol. 75.
(enero 2020)
ORIGINAL ARTICLE
Open Access
SELAdb: A database of exonic variants in a Brazilian population referred to a quaternary medical center in São Paulo
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Antonio Marcondes LerarioI,II,IX, Dipika R. MohanIII,IV, Luciana Ribeiro MontenegroI,IX, Mariana Ferreira de Assis FunariI,IX, Mirian Yumie NishiI,IX, Amanda de Moraes NarcizoIX, Anna Flavia Figueredo BenedettiIX, Sueli Mieko Oba-ShinjoVII,IX, Aurélio José VitorinoVIII, Rogério Alexandre Scripnic Xavier dos SantosVIII, Alexander Augusto de Lima JorgeI,IX, Luiz Fernando OnuchicV,VI,IX, Suely Kazue Nagahashi MarieVII,IX,#, Berenice Bilharinho MendoncaI,IX,
,#
I Disciplina de Endocrinologia e Metabologia, Departamento de Clinica Medica, LIM/42, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR
II Department of Internal Medicine, Division of Endocrinology, Metabolism and Diabetes, University of Michigan, Ann Arbor, MI, USA
III Medical Scientist Training Program, University of Michigan, Ann Arbor, MI, USA
IV Doctoral Program in Cancer Biology, University of Michigan, Ann Arbor, MI, USA
V Disciplina de Medicina Molecular, Departamento de Clinica Medica, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo, SP, BR
VI Disciplina de Nefrologia, Departamento de Clinica Medica, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo, SP, BR
VII Laboratorio de Biologia Molecular e Celular, LIM/15, Departamento de Neurologia, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo, SP, BR
VIII Laboratorio de Informatica Medica - LIM/01, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo, SP, BR
IX Laboratorio de Sequenciamento em Larga Escala (SELA), Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo, SP, BR
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OBJECTIVES:

High-throughput sequencing of genomes, exomes, and disease-focused gene panels is becoming increasingly common for molecular diagnostics. However, identifying a single clinically relevant pathogenic variant among thousands of genetic polymorphisms is a challenging task. Publicly available genomic databases are useful resources to filter out common genetic variants present in the population and enable the identification of each disease-causing variant. Based on our experience applying these technologies at Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP), São Paulo, Brazil, we recognized that the Brazilian population is not adequately represented in widely available genomic databases.

METHODS:

Here, we took advantage of our 5-year experience as a high-throughput sequencing core facility focused on individuals with putative genetic disorders to build a genomic database that may serve as a more accurate reference for our patient population: SELAdb.

RESULTS/CONCLUSIONS:

Currently, our database comprises a final cohort of 523 unrelated individuals, including patients or family members managed by different clinics of HCFMUSP. We compared SELAdb with other publicly available genomic databases and demonstrated that this population is very heterogeneous, largely resembling Latin American individuals of mixed origin, rather than individuals of pure European ancestry. Interestingly, exclusively through SELAdb, we identified a spectrum of known and potentially novel pathogenic variants in genes associated with highly penetrant Mendelian disorders, illustrating that pathogenic variants circulating in the Brazilian population that is treated in our clinics are underrepresented in other population databases. SELAdb is freely available for public consultation at: http://intranet.fm.usp.br/sela

KEYWORDS:
Next Generation Sequencing
Database
Mendelian Disorders
Brazil
Population Genetics
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INTRODUCTION

Advances in high-throughput sequencing technologies in the last decade have enabled systematic genome profiling of thousands of individuals from diverse populations worldwide. Consequently, we now have several publicly available genomic databases that illustrate the profound variability across different ethnic groups, serving as rich resources for investigators seeking to elucidate the molecular basis of human diseases. Indeed, as the cost of next-generation sequencing (NGS) is rapidly declining, NGS-based approaches, including exome sequencing and disease-focused gene panels, are quickly becoming the mainstay of molecular diagnostics.

For example, a clinician who has identified a patient with phenotypic features resembling a particular familial syndrome in the absence of family history may choose to perform exome sequencing using the genomic DNA from this patient, in hopes of identifying a disease-causing genetic variant. However, such an approach routinely results in the identification of thousands of variants. Defining which of these variants is the true disease-causing allele is clinically challenging. An approach that is usually adopted to overcome this obstacle is to filter out common variants in a population. However, even this approach has limited utility, as certain populations are underrepresented in many populational databases, and the clinically relevant allele frequencies remain uncharacterized. At Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HCFMUSP), São Paulo, Brazil, we adopt NGS-based methods for the diagnosis of putative Mendelian disorders, as a standard practice. However, the most widely utilized databases (e.g. The Genome Aggregation Database (gnomAD) (1), 1000 Genomes (2)) have poor annotation and an underrepresentation of diverse individuals of South American origin (3).

The population of Brazil, in particular, is comprised of many different ethnic groups (3,4), and provides a unique opportunity to identify novel uncharacterized disease-causing variants that are unique to this population. Illustrating this point, an unusually high incidence of pediatric adrenocortical tumors in Brazil (nearly twenty times higher than the global incidence) led to the identification of a novel variant in TP53 (p.R337H) present in nearly 0.3% of the Southeastern Brazilian population and accounting for 90% of pediatric adrenal tumors in this area (5–7). Subsequent studies demonstrated that the high prevalence of p.R337H in this population can be attributed to a founder effect (5), and highlights the critical need of regionally focused population genetics studies. Recently, investigators at the University of São Paulo Institute of Biology developed a database of exomes from 609 elderly individuals, Arquivo Brasileiro Online de Mutações (ABraOM), which serves as a powerful resource for clinicians and investigators researching this group of individuals (8). Our goal at HCFMUSP was to take advantage of our expertise as an NGS-based facility, with half a decade of experience in using exome sequencing as a diagnostic tool, to create a more representative database of our general patient population.

MATERIALS AND METHODS

Subjects. Our database includes 862 individuals associated with different clinical services (Endocrinology, Neurology, Nephrology, Psychiatric, Gastroenterology, and Rheumatology) at HCFMUSP, comprised of patients with putative Mendelian disorders of uncertain or unknown genetic causes, patients with complex disease traits, patients with sporadic tumors, or unaffected family members. We applied a kinship filter (9) to remove related individuals to produce a final cohort of 523 unrelated Brazilian individuals (240 males, 283 females).

Sequencing. Exome sequencing was performed using the Illumina HiSeq 2500 platform in Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ - 2013-2014) or Laboratório de Sequenciamento em Larga Escala da Faculdade de Medicina da Universidade de São Paulo (SELA-FMUSP - 2014-2019). Library preparation and exome capture were performed using the Nextera Rapid Capture Enrichment (Illumina, San Diego, CA) or the SureSelect Target Enrichment System All Exon +/- UTRs V4, V5, and V6 (Agilent Technologies, Santa Clara, CA), according to the manufacturer's instructions.

Bioinformatics analysis. After quality-control using FastQC(10) and adapter sequencing removal using the bbduk tool from bbmap (11), paired-end reads were aligned to the hg19/GRCh37+decoy version of the human genome using bwa-mem (12). Aligned reads were then coordinate-sorted, deduplicated, and indexed using bamsort and bammarkduplicate tools from biobambam2 (13). Sequencing errors, duplication rates, and coverage metrics were assessed using qualimap (14). For variant calling, we used an incremental joint variant calling strategy based on freebayes (15), as follows: first, we used freebayes to perform variant calling on each bam file, individually. We filtered out low-coverage and low-quality variants (<10x and QUAL<10, respectively). We performed multiallelic sites decomposition, multiallelic variants decomposition, and left normalization of InDels using vt decompose, vcfallelicprimitives (from vcflib), and vt normalize tools (16,17). After processing all vcf files, we created a joint list of variants using the vcfoverlay (16) tool from vcflib. We then used this joint list as an input for freebayes (using the -@ option) to perform a second round of variant calling. We used the “-l” switch from freebayes to restrict calls only to positions of variants reported in the joint list. This final step enabled us to report all sites from the joint list, even in the samples bearing the reference allele. Sex-specific ploidy was set to regions outside the pseudo-autosomal region of the Y chromosome. Next, we used bcftools merge (18) to combine the variant information from individual vcf files into a single file containing the sex-corrected allele frequencies for all the variants detected in the dataset. We then used hail (9) to remove related individuals from the dataset. Finally, we used SNPEff and SNPSift(19,20) to annotate these variants according to the genomic loci, the functional consequences on protein-coding genes, and dbSNP membership. A summary of the bioinformatics workflow employed to construct Sequenciamento em Larga Escala database (SELAdb) is depicted in Figure 1. Finally, we used somalier (21) to estimate the ancestry of our population in comparison with the populations included in the 1000 Genomes project, using the individuals in 1000 Genomes as a training set. We represented these ancestry data using principal component analysis (PCA) plots built using ggplot2 (22) and used a neural network classifier (21) to calculate posterior probabilities for the assignment of a sample in our cohort to the populations defined in 1000 Genomes.

Figure 1.

Flow chart of data processing steps used to generate SELAdb database.

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Ethics

This study was approved by the Institutional Review Board of HCFMUSP, in accordance with the provisions in the Helsinki Declaration. Written informed consent was obtained from all individuals included in SELAdb.

RESULTSSELAdb accurately captures the diversity of the southeastern Brazilian population

As detailed in Figure 1, we analyzed exome sequencing data from 862 individuals to generate a final cohort of exomes from 523 unrelated individuals (240 males, 283 females) and a corresponding list of annotated variants with allele frequencies, which we deposited in a novel Brazilian population database, SELAdb. Among these 523 individuals, we identified 1,788,789 variants, including 1,615,436 single-nucleotide variants, 47,805 small insertions, 121,255 small deletions, and 4,293 complex substitutions (Table 1). Among these variants, 502,738 (28.1%) are not reported in dbSNP151 and, therefore, may represent novel variants present in the Brazilian population (Table 1). A total of 2,973,280 effects (alterations resulting in potential changes in protein structure, function, and/or expression, such as amino acid changes or abrogation of transcription factor binding sites) could be attributed to these 1,788,789 variants, including 581,757 targeting annotated exons. Included among these are 152,984 synonymous, 207,854 missense, 3,742 stop-gained, 256 stop-lost, 405 start-lost, and 9,653 frameshift variants (Table 2).

Table 1.

SELAdb variants classified by type and presence in other databases.

Total number of SELAdb variants  1,788,789 
Variant type  Number of variants (% of SELAdb) 
Single nucleotide polymorphism (SNP)  1,615,436 (90.3%) 
Insertion  47,805 (2.7%) 
Deletion  121,255 (6.8%) 
Mixed  4,293 (0.2%) 
Database  Number of variants annotated (% of SELAdb)Number of novel variants (% of SELAdb) 
dbSNP151  Annotated - 1,286,051 (71.9%)Novel - 502,738 (28.1%) 
1000 Genomes  Annotated - 1,205,879 (67.4%)Novel - 582,910 (32.6%) 
gnomAD  Annotated - 489,563 (27.4%)Novel - 1,299,226 (72.6%) 
ABraOM  Annotated - 915,702 (51.2%)Novel - 873,087 (48.8%) 
Table 2.

Number of effects attributed to variants in SELAdb by region and type.

Region  Count  Percentage (%) 
Downstream  402,130  13.525 
Exon  581,757  19.566 
Gene  16  0.001 
Intergenic  60,291  2.028 
Intron  1,091,611  36.714 
Motif  8,440  0.284 
Splice site acceptor  1,387  0.047 
Splice site donor  1,604  0.054 
Splice site region  35,011  1.178 
Transcript  199,903  6.723 
Upstream  374,792  12.605 
UTR 3 prime  165,649  5.571 
UTR 5 prime  50,689  1.705 
Type  Count  Percentage (%) 
3' UTR variant  165,652  5.941 
5' UTR premature start codon gain variant  6,053  0.201 
5' UTR truncation 
5' UTR variant  44,637  1.48 
TFBS ablation  75  0.002 
TFBS variant  8,365  0.277 
Bidirectional gene fusion 
Conservative inframe DEL  1,150  0.038 
Conservative inframe INS  480  0.016 
Disruptive inframe DEL  2,133  0.071 
Disruptive inframe INS  655  0.022 
Downstream gene variant  402,132  13.331 
Exon loss variant 
Frameshift variant  9,653  0.32 
Gene Fusion 
Initiator codon variant  38  0.001 
Intergenic variant  60,291  1.999 
Intragenic variant  174,620  5.789 
Intron variant  1,123,430  37.241 
Missense variant  207,854  6.89 
Noncoding transcript exon variant  164,421  5.45 
Noncoding transcript variant  90  0.003 
Protein-protein contact  476  0.016 
Sequence feature  25,193  0.835 
Splice acceptor variant  1,414  0.047 
Splice donor variant  1,675  0.056 
Splice region variant  40,619  1.347 
Start lost  405  0.013 
Stop gained  3,742  0.124 
Stop lost  256  0.008 
Stop retained variant  123  0.004 
Structural interaction variant  43,197  1.432 
Synonymous variant  152,984  5.071 
Upstream gene variant  374,792  12.424 

UTR=untranslated region, TFBS=transcription factor binding site, DEL=deletion, INS=insertion.

Then, we sought to determine the ancestry of the individuals in our cohort using the populations defined by the 1000 Genomes project as a reference. The biplot shown in Figure 2 represents the first two principal components that capture most of the variability present in the data. The European (EUR), African (AFR), and East Asian (EAS) populations encompass three well-defined clusters in the extremes of this two-dimensional space, forming the vertices of a triangle. As expected, the admixed American population (AMR) exhibits higher variability and is dispersed over a larger area of the plot, with limbs extending to the areas delimited by the EUR, AFR, and EAS populations (Figure 2). Similar to previous studies, our results indicate that SELAdb, comprising urban southeastern Brazilian individuals, forms a continuum between EUR and AFR populations, which is consistent with a high degree of intermarriage between these two populations in southeastern Brazil (3). Furthermore, we observe here that individuals in SELAdb broadly overlap with a large fraction of the AMR population (Figure 2). To better quantify and define the putative ancestry of individuals in SELAdb, we applied a neural network classifier to fit individuals in SELAdb to pre-existing EAS, SAS, EUR, AFR, and AMR categories in 1000 Genomes (Figure 3). This analysis revealed that the majority of individuals in SELAdb could, indeed, be classified as AMR (75.5%), followed by EUR (18.0%), AFR (5.2%), and EAS (1.3%) (Figure 3B).

Figure 2.

Biplot depicting the two principal components (x-axis: PC1, y-axis: PC2) capturing the highest genetic variation among different populations from 1000 Genomes (colored circles) and SELAdb individuals (red crosses). Each circle or cross represents a single individual. The populations represented by the colored circles are African (AFR), Ad-Mixed American (AMR), East Asian (EAS), European (EUR), and South Asian (SAS).

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Figure 3.

Classification of SELAdb individuals using a neural network classifier and 1000 Genomes populations as the training set. Panel A depicts a series of biplots (x-axis: PC1, y-axis: PC2) representing the PCA analysis of SELAdb individuals, which are color-coded according to the posterior probabilities of being classified as a distinct population by using 1000 Genomes, including AFR (brown, upper left), AMR (blue, upper right), EAS (yellow, lower left), and EUR (green, lower right). Panel B depicts a biplot (left; x-axis: PC1, y-axis: PC2) representing each SELAdb individual, which is color-coded according the final classification given by the neural network classifier. The final distribution of individuals in each category are represented in the donut plot (right). Consistent with the distribution of SELAdb individuals in Panel A, most SELAdb individuals are classified as AMR.

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The AMR population in 1000 Genomes is a heterogeneous group comprised of individuals from different geographic regions of Latin America, but does not include any Brazilian individuals (2). Given that the majority of individuals in SELAdb is classified as AMR, despite the absence of Brazilian individuals in this set, we sought to determine which subgroup of the AMR population in 1000 Genomes is the most similar to individuals from SELAdb. To address this question, we performed a similar PCA analysis, but this time with the AMR population separated into its constituent subpopulations, including Peruvians of Lima (PEL), Mexicans of Los Angeles (MXL), Colombians (CLM), and Puerto Ricans (PUR) (Figure 4A). From this analysis, we can appreciate that the AMR population is comprised of unique subclusters, in which PEL, MXL, CLM, and PUR populations cluster separately within the region spanned by AMR in Figure 2. This observation suggests that the broad space encompassed by AMR in the biplot may be partially explained by the number of populations of diverse origins defined as AMR, rather than by a broad heterogeneity dispersed throughout each population. Indeed, Figure 4A illustrates that the MXL and PEL form a distinct cluster that localizes closer to EAS, and farther from EUR and AFR; this observation is consistent with the theory of the Asian origin of native American populations (23). In contrast, PUR exhibit a distinct pattern, characterized by a higher influence of both EUR and AFR. Finally, CLM exhibit characteristics of both MXL/PEL and PUR. We observe here that SELAdb largely overlaps with PUR, which is consistent with the stronger influence of EUR and AFR in the southeastern Brazilian population (3). By further quantifying this overlap using a neural network classifier and the subdivided AMR groups (Figure 4B), we could observe that the majority of individuals in SELAdb are classified as PUR (60.2%). This observation suggests a similar contribution of European and African ancestries to both PUR and the southeastern Brazilian populations, which is consistent with common historical aspects of colonization of both geographical regions and recent studies defining the ancestral contributions to each population (3,24).

Figure 4.

Panel A: biplot (x-axis: PC1, y-axis: PC2) showing the overlap between 1000 Genomes (colored circles) and SELAdb individuals (red crosses). In this representation, the AMR population is split according to its subpopulations, including Colombians (CLM), Mexicans of Los Angeles (MXL), Peruvians of Lima (PEL), and Puerto Ricans (PUR). Panel B: Neural network classification of SELAdb individuals using the 1000 Genomes as a training set. Consistent with the distribution of SELAdb individuals in Panel A, most SELAdb individuals classify as PUR.

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SELAdb enables identification of novel, potentially disease-causing variants in a Brazilian population

Given the ability of SELAdb to identify novel variants present in the Brazilian population, that are distinct from other population databases (Table 1), we sought to evaluate its utility in identifying well documented pathogenic variants in a set of 60 genes associated with highly penetrant genetic disorders according to the American College of Medical Genetics and Genomics (ACMG SF v2.0) (25). We were particularly interested in this set of genes, as variants are related with a high risk of diseases associated with early mortality, including cardiovascular disease and familial neoplasia syndromes. Therefore, we suspected that many of these variants may be unique to the Brazilian population but absent from ABraOM database, given its inclusion criteria (8).

We identified 24 known pathogenic or likely pathogenic variants according to ClinVar (26) (Table 3). Interestingly, 11 of these variants are exclusive to SELAdb, not being reported in gnomAD and ABraOM. Thirteen variants were also reported in gnomAD and/or ABraOM (7 in gnomAD and ABraOM, 6 in gnomAD only, 0 in ABraOM only). In addition, we identified 7 variants that were predicted to be pathogenic, according to InterVar (27), among which only 4 were present in gnomAD and/or ABraOM (Table 4). These observations highlight the unique contribution of SELAdb in augmenting the spectrum of potential disease-causing variants present in the Brazilian population and illustrate its clinical and research utility.

Table 3.

SELAdb variants classified as pathogenic/likely pathogenic using ClinVar and recommended to be reported by ACMG.

Gene  Mutation  dbSNP151  SELAdb allele frequency  Other databases 
MUTYH  NM_001128425:p.479_480del  rs587778541  1/1042  gnomAD, ABraOM 
MUTYH  NM_001128425:p.Gly396Asp  rs36053993  6/1044  gnomAD, ABraOM 
PCSK9  NM_174936:p.Tyr142X  rs67608943  1/1046  gnomAD, ABraOM 
LMNA  NM_001282625:p.Cys522X  N/A  1/1044  SELAdb only 
TNNT2  NM_001001430:p.Asn271Ile  N/A  1/1046  SELAdb only 
PKP2  NM_004572:c.2578-2A>C  N/A  1/1016  SELAdb only 
BRCA2  NM_000059:p.Tyr2154fs  rs80359596  1/970  SELAdb only 
ATP7B  NM_000053:exon17:c.A3694C:p.T1232P  rs568009639  1/1046  gnomAD, ABraOM 
ATPB7  NM_000053:p.Pro1134fs  rs137853281  2/1044  gnomAD, ABraOM 
ATPB7  NM_000053:p.His1069Gln  rs76151636  1/1046  gnomAD 
ATPB7  NM_000053:p.Pro840Leu  rs768671894  1/1046  SELAdb only 
ATPB7  NM_000053:p.Leu708Pro  rs121908000  1/1034  SELAdb only 
BRCA1  NM_007294:p.Cys903X  N/A  2/1030  SELAdb only 
BRCA1  NM_007294:p.Cys64Arg  rs80357064  1/1004  SELAdb only 
LDLR  NM_000527:p.Gly55Gly  rs150644181  1/1046  gnomAD, ABraOM 
LDLR  NM_000527:p.Glu418Lys  N/A  1/1038  gnomAD 
LDLR  NM_000527:p.Gly592Glu  rs137929307  1/1038  gnomAD 
LDLR  NM_000527:p.Arg744X  rs200793488  1/1046  SELAdb only 
TNNI3  NM_000363:p.Asp196Asn  rs104894727  1/1042  gnomAD 
TNNI3  NM_000363:p.Arg145Gln  rs397516349  1/1026  gnomAD 
APOB  NM_000384:p.Ala13fs  N/A  2/1008  SELAdb only 
APOB  NM_000384:p.F2181fs  N/A  1/916  gnomAD 
PMS2  NM_000535:c.989-2A>G  rs587779347  1/890  SELAdb only 
PMS2  NM_000535:p.Met1Val  rs587779333  1/1032  gnomAD, ABraOM 

NOTE. Regarding the BRCA1 mutation NM_007294:p.Cys903X, with allele frequency 2/1030: both alleles are present in the same individual in SELAdb. The phenotype of this individual is described in Freire et al. Eur. J. Med. Genet. 2018 (PMID: 29133208).

Table 4.

SELAdb variants that were classified as pathogenic using InterVar and recommended to be reported by ACMG.

Gene  Mutation  dbSNP151  SELAdb allele frequency  Other databases 
PCSK9  NM_174936:p.Gln387X  N/A  1/1020  SELAdb only 
CACNA1S  NM_000069:p.Arg1702X  rs550371466  1/1044  gnomAD 
RYR2  NM_001035:p.Cys1914X  N/A  1/1030  SELAdb only 
SCN5A  NM_198056:p.Glu1053Lys  rs137854617  1/1010  gnomAD, ABraOM 
RET  NM_020630:p.Val804Met  rs79658334  1/934  gnomAD, ABraOM 
MYBPC3  NM_000256:p.Gly278X  N/A  1/978  SELAdb only 
ATP7B  NM_000053:p.Gly170X  N/A  1/1022  gnomAD 
DISCUSSION

The Brazilian population is highly admixed, being comprised of several different ethnic groups, and inadequately represented in publicly available genomic databases (3,4). Here, we took advantage of our 5-year experience as a large-scale sequencing core facility to build a representative local genomic database for the southeastern Brazilian population, SELAdb. Although many individuals included in SELAdb are patients or family members with rare Mendelian disorders, contributing to the identification of novel disease-causing variants (28–40), our analyses demonstrate that it adequately represents our local patient population. Through ancestry analysis, we observed that the population captured by SELAdb bears diverse genetic influences, which are characteristic of the admixed southeastern Brazilian population, similar to previous reports (3). In this analysis, we also identified a large overlap between SELAdb individuals and the 1000 Genomes AMR population (Figures 2-3), especially regarding PUR individuals (Figure 4). Taken together, our analyses illustrate the importance of regional population databases in better representing individuals of diverse origin.

Our effort adds value to another recently launched genetic database on Brazilian individuals, ABraOM (8). However, given the focus of ABraOM on healthy elderly individuals, pathogenic variants that are present in our patient population may be underrepresented. In contrast, our database is focused on a population of patients or family members with putative genetic diseases in whom we have identified a spectrum of known and potentially novel pathogenic variants, as illustrated in Tables 3 and 4. We believe that SELAdb may be informative regarding the prevalence of pathogenic variants in the southeastern Brazilian population and facilitate future genetics studies on Brazilian individuals.

In conclusion, SELAdb is a publicly available database that is representative of our regional patient population. We believe that, in addition to ABraOM, SELAdb will be a valuable resource for investigators using genomics data from the Brazilian population. SELAdb is rapidly increasing in size; updates and improvements, including more detailed phenotypic annotations associated with specific variants, are expected to be implemented every six months. The data can be freely accessed at http://intranet.fm.usp.br/sela.

AUTHOR CONTRIBUTIONS

Lerario AM was responsible for the conceptualization, data analysis, manuscript writing and supervision. Mohan DR was responsible for the data analysis and manuscript writing. Benedetti AFF was responsible for the data analysis and website construction. Montenegro LR, Funari MFA, Nishi MY, Narcizo AM, Oba-Shinjo SM were responsible for the data generation. Vitorino AJ, Santos RASX were responsible for the website and database construction. Jorge AAL, Onuchic LF, Marie SKN and Mendonça BB were responsible for the project coordination, supervision and manuscript editing.

ACKNOWLEDGMENTS

This work was supported by grants from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Grants 2013/02162-8 and 2014/50137-5).

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No potential conflict of interest was reported.

Contributed equally to this study.

Copyright © 2020. CLINICS
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