metricas
covid
Buscar en
Endocrinología, Diabetes y Nutrición (English ed.)
Toda la web
Inicio Endocrinología, Diabetes y Nutrición (English ed.) The rs483145 polymorphism of MC4R gene is not associated with obesity in the Chi...
Información de la revista
Vol. 69. Núm. 4.
Páginas 254-261 (abril 2022)
Visitas
984
Vol. 69. Núm. 4.
Páginas 254-261 (abril 2022)
Original article
Acceso a texto completo
The rs483145 polymorphism of MC4R gene is not associated with obesity in the Chilean population: Results of GENADIO study
El polimorfismo rs483145 del gen MC4R no se asocia con obesidad en población chilena: resultados del estudio GENADIO
Visitas
984
Lorena Mardonesa, Esteban Parra-Valenciab, Fanny Petermann-Rochac,d, María Adela Martínez-Sanguinettie, Ana María Leiva-Ordoñezf, Nicole Lasserre-Lasog, Miquel Martorellh,i, Natalia Ulloah,j, Eduardo Sanhuezaa, Francisco Pérez-Bravok, Carlos Celis-Moralesc,l,m, Marcelo Villagrána,
Autor para correspondencia
marcelo.villagran@ucsc.cl

Corresponding author.
, on behalf of the ELHOC research group (Epidemiology of Lifestyle, Health Outcomes in Chile)
a Laboratorio de Investigación en Ciencias Biomédicas, Facultad de Medicina, Universidad Católica de la Santísima Concepción, Concepción, Chile
b Departamento de Ciencias Clínicas y Pre-Clínicas, Facultad de Medicina, Universidad Católica de la Santísima Concepción, Concepción, Chile
c BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, UK
d Institute of Health and Wellbeing, University of Glasgow, Glasgow, UK
e Instituto de Farmacia, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile
f Instituto de Anatomía, Histología y Patología, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile
g Escuela de Nutrición y Dietética, Facultad de Salud, Universidad Santo Tomas, Región Metropolitana, Chile
h Centro de Vida Saludable, Universidad de Concepción, Concepción, Chile
i Departamento de Nutrición y Dietética, Facultad de Farmacia, Universidad de Concepción, Concepción, Chile
j Departamento de Bioquímica Clínica e Inmunología, Facultad de Farmacia, Universidad de Concepción, Concepción, Chile
k Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Santiago, Chile
l Centro de Investigación en Fisiología del Ejercicio (CIFE), Universidad Mayor, Santiago, Chile
m Laboratorio de Rendimiento Humano, Grupo de Estudio en Educación, Actividad Física y Salud (GEEAFyS), Universidad Católica del Maule, Talca, Chile
Ver más
Este artículo ha recibido
Información del artículo
Resumen
Texto completo
Bibliografía
Descargar PDF
Estadísticas
Tablas (3)
Table 1. Population characteristics by genotype of the MC4R gene (rs483145).
Table 2. Frequency of the MC4R genotype (rs483145).
Table 3. Association between the genotype of the MC4R gene (rs483145) and markers of obesity.
Mostrar másMostrar menos
Abstract
Introduction

The melanocortin receptor 4 (MC4R) participates in the control of appetite at the level of the central nervous system, through the leptin-melanocortin pathway. An association between different polymorphisms of the MC4R gene and obesity has been reported. However, there are few studies of the rs483145 single nucleotide polymorphism (SNP) of this gene.

Objective

To investigate its prevalence and association with adiposity markers in Chilean adults.

Methods

The prevalence of SNP rs483145, of the MC4R gene, was determined in 259 participants of the GENADIO study (genes, environment, diabetes and obesity) by means of real-time polymerase chain reaction (PCR). The association between the risk allele of MC4R (A) and adiposity markers (body weight, body mass index, fat mass percentage, hip circumference, waist circumference, waist-to-hip ratio) was performed by linear regression analysis and adjusted for confusion variables (socio-demographic and physic activity) using three statistical models.

Results

It was determined that the prevalence of the risk allele (A) of the SNP rs483145 of the MC4R gene is 24.5% in the Chilean adult population included in this study, without finding an association with any of the adiposity markers studied, both in adjusted and unadjusted models.

Conclusion

The presence of the risk allele of SNP rs483145 of the MC4R gene is not associated with adiposity markers in the Chilean adult population studied. New studies with a bigger sample size will be necessary to confirm these results.

Keywords:
MC4R
rs483145
Obesity
BMI
Resumen
Antecedentes

El receptor de melanocortina 4 (MC4R) participa en el control del apetito a nivel del sistema nervioso central, a través de la vía de la leptina-melanocortina. Se ha reportado asociación entre diferentes polimorfismos del gen MC4R y la obesidad; sin embargo, existen escasos estudios del polimorfismo de nucleótido simple (SNP) rs483145 de este gen.

Objetivo

Investigar su prevalencia y asociación con marcadores de adiposidad en adultos chilenos.

Métodos

La prevalencia del SNP rs483145, del gen MC4R, fue determinada en 259 participantes del estudio Genes, Ambiente, Diabetes y Obesidad (GENADIO) mediante reacción en cadena de la polimerasa (PCR) en tiempo real. La asociación del alelo de riesgo de MC4R (A) con marcadores de adiposidad (peso corporal, índice de masa corporal, porcentaje de masa grasa, perímetro de cadera, perímetro de cintura e índice cintura/cadera), se realizó mediante análisis de regresión lineal y fue ajustada por variables de confusión (sociodemográficas y de actividad física) mediante 3 modelos estadísticos.

Resultados

Se determinó que la prevalencia del alelo de riesgo (A) del SNP rs483145 del gen MC4R es del 24,5% en la población adulta chilena incluida en este estudio, sin encontrar asociación con ninguno de los marcadores de adiposidad estudiados, tanto en modelos ajustados como sin ajustar.

Conclusión

La presencia del alelo de riesgo del SNP rs483145 del gen MC4R no se asocia con marcadores de adiposidad en la población adulta chilena estudiada. Nuevos estudios que incluyan una muestra más numerosa serán necesarios para confirmar estos resultados.

Palabras clave:
MC4R
rs483145
Obesidad
IMC
Texto completo
Introduction

In recent years, obesity has become one of the most significant public health problems worldwide, accounting for more than 2.6 million deaths per year.1,2 It was initially recognised only as a risk factor for ischaemic cardiomyopathy; later, however, it was linked to diseases such as diabetes mellitus type 2, hypertension and cancer.3 Currently, 34.4% of the Chilean population is obese, and 74.2% of the adult population is overweight.4

The development of obesity is multifactorial, and combines modifiable factors, such as nutrition and physical activity, with non-modifiable factors, such as age, sex and genetic factors.5,6 At present, more than 700 genetic markers associated with common obesity have been reported, including single-nucleotide polymorphisms (SNPs) associated with the FTO gene, as they show the greatest strength of association, and melanocortin-4 receptor (MC4R) SNPs, as they were among the first to be identified.7,8

MC4R is a G-protein–coupled receptor linked to appetite control through the hypothalamic leptin–melanocortin pathway.9 Activation of MC4R occurs through its binding to molecules derived from pro-opiomelanocortin (POMC), such as the α and β forms of melanocyte-stimulating hormone (αMSH and βMSH).10 Agouti-related peptide (AGRP) is another MC4R-binding peptide that blocks anorexigenic signalling by POMC derivatives.11 Consequently, research has been conducted on the potential use of some synthetic MC4R agonists as drugs to treat obesity, especially in syndromic obesity of monogenic origin.12 Although the first agonists had adverse cardiovascular effects,13 subsequent phase-3 clinical studies of setmelanotide demonstrated significant appetite reduction and weight loss in patients with POMC or leptin receptor (LEPR) deficiency, paving the way for clinical use.14,15

Elimination of MC4R through genetic modification in mice causes obesity associated with hyperphagia and lack of satiety, while in humans mutations with loss of function are associated with severe early-onset monogenic obesity.16,17 These MC4R-inactivating mutations cause increases in body weight by up to 7 kg in homozygous individuals, but show a prevalence below 0.1% in Caucasian populations.18 In addition, MC4R has other high-prevalence genetic variants corresponding to SNPs, some of which are associated with a modest increase in the risk of obesity.15MC4R SNPs are distributed throughout the entire gene, including rs483145 and rs11872992 located in the promoter region, rs2229616 (V103I) and rs52820871 (I251 L) present in the only exon, and rs17782313 and rs12970134 located 188 kb and 150 kb from the 3′ end of the gene (distal locus), respectively.19 The latter SNPs are the most extensively studied and show an association with an increased risk of obesity in Asian, European and American people, reaching a variation by up to 0.3 kg/m2 in body mass index (BMI) per risk allele copy.20,21 By contrast, for the rs483145 SNP, just one study of association has been conducted, in a population of Pima Indians (indigenous people from Arizona, United States). This study found an increase in BMI by 0.58 kg/m2 per risk allele copy and a risk allele frequency over 80%.22 In the absence of studies linking the prevalence of the MC4R rs483145 SNP to obesity in the adult Chilean population, the objective of this study was to determine its prevalence in said population and to investigate its possible association with markers of adiposity.

Materials and methods

A cross-sectional descriptive study was conducted that included 259 individuals with available data for the genotype of the rs483145 SNP in the MC4R gene, belonging to the Genes, Ambiente, Diabetes y Obesidad [Genes, Environment, Diabetes and Obesity] (GENADIO) study, conducted in Chile from 2009 to 2011. The GENADIO project studied a total population of 475 individuals of Mapuche or European descent (249 and 226 individuals, respectively) from the Biobío and Los Ríos regions of Chile who had no history of metabolic or cardiovascular disease and who, at the time of the evaluation, had no prescription medications.23 The sample size was calculated based on CENSO 2002, the 2002 Chilean census, according to which indigenous people represented 4.6% of the country's population. To select participants of Mapuche or European descent and exclude individuals of mixed-race ancestry, only those whose paternal and maternal surnames were both of Mapuche or European origin, respectively, were included. In addition, to select Mapuche individuals, only those whose blood group was O were included. Participants were recruited by means of open invitations to the community or through community organisations such as primary healthcare centres, schools and social clubs. The study was approved by the independent ethics committees of the Universidad de Chile [University of Chile], Universidad de Concepción [University of Concepción] and University of Glasgow, who adhered to the Declaration of Helsinki on studies in humans. All participants signed an informed consent form prior to data collection.

Determination of allelic variants of the MC4R gene

To genotype the rs483145 SNP of the MC4R gene, genomic DNA was obtained from peripheral leukocytes using the QIAamp® DNA Blood Midi Kit (QIAGEN, Ltd. UK). Allelic discrimination was performed by means of real-time polymerase chain reaction (PCR) in an ABI 7900 H T thermal cycler using TaqMan® probes (Applied Biosystems, Warrington, UK). All sample testing was performed in duplicate, with a rate of success of genotype determination of 98%.

Markers of adiposity

Anthropometric evaluation was performed by a trained professional using standardised protocols.24 Body weight and height were measured using electronic scales (Tanita TBF-300A, United States) and a stadiometer (SECA Model A800, United States) with a precision of 100 g and 1 mm, respectively. Nutritional status was classified based on the cut-off points suggested by the World Health Organization (WHO): underweight: <18.5 kg/m2; normal weight: 18.5–24.9 kg/m2; overweight: 25.0–29.9 kg/m2 and obesity: ≥30.0 kg/m2. Waist circumference (WC) was measured using non-stretch metric measuring tape (SECA Model 201, United States). The values used to define central obesity were the following: WC ≥ 94 cm in men and ≥80 cm in women.25 Hip circumference was measured around the fullest part of the hip region, at approximately the height of the pubic symphysis. Waist-to-hip ratio, determined by dividing waist circumference by hip circumference, was used to measure abdominal fat. Body composition was determined by measuring four skinfolds (biceps, subscapular, suprailiac and triceps) using a Harpenden calliper (Cranlea & Company, UK), with a precision of 0.2 mm.23,26 The Durnin–Womersley formula was used to estimate body fat percentage.26

Sociodemographic variables and physical activity

Sociodemographic data (age, sex, area of residence, ethnicity and level of education) were collected through validated surveys.19 Levels of physical activity (PA) and sedentary time were estimated by physical activity accelerometry (ActiGraph GT1M, United States). PA intensity and energy expenditure were determined using Freedson's algorithm.27

Statistical analysis

The data characterising the population studied are presented in terms of mean and standard deviation (SD) for continuous variables and percentage for categorical variables. Genotype differences were determined using regression analysis for continuous variables and the χ2 test for categorical variables. To investigate the link between the rs483145 polymorphism of the MC4R gene and markers of obesity (body weight, BMI, WC, hip circumference, waist-to-hip ratio and body fat percentage), linear regression analysis was performed. In addition, analyses of interaction between MC4R and sex and between MC4R and ethnicity were performed in order to determine whether the link between MC4R and markers of adiposity were different for men and women or for the Mapuche and non-Mapuche population. Since no evidence of a significant interaction was found (sex: p = 0.369; ethnicity: p = 0.560), it was not necessary to stratify the analyses of association by sex or ethnicity.

The rs483145 SNP genotype of the MC4R gene was coded according to an additive genetic model, where: 0 = homozygous TT for the protective allele, 1 = heterozygous AT for the risk allele and 2 = homozygous AA for the risk allele.

All analyses were adjusted for confounding variables using three statistical models: Model 0: not adjusted; Model 1: adjusted for age, sex, ethnicity, level of education and area of residence (urban/rural); and Model 2: adjusted for Model 1, but also for physical activity. The STATA® SE v14 software programme was used for all analyses. The level of significance was set at p < 0.05.

Results

The general characteristics of the study population by genotype are found in Table 1. The three genotypes had a similar average age: 35.8 for TT, 37.6 for TA and 34.0 for AA. It was found that 66.6% of those with the TA genotype, 51.6% of those with the TT genotype and 47.0% of those with the AA genotype were female. Lower rates of Mapuche ethnicity were seen among individuals with risk genotypes (34.4% for TA and 35.2% for AA) versus carriers of the protective TT genotype (44.9%). No appreciable between-group differences were seen in other sociodemographic variables or in parameters of physical activity. Table 2 shows the allele frequencies of the rs483145 SNP, distributed according to the Hardy–Weinberg equilibrium (χ2 = 0.616) and corresponding to 0.754 for the protective allele (T) and 0.245 for the risk allele (A).

Table 1.

Population characteristics by genotype of the MC4R gene (rs483145).

  TT  TA  AA 
No.  149  93  17 
Age (years)  35.8 ± 12.6  37.6 ± 13.1  34.0 ± 14.5 
Sex, female (%)  51.68  66.67  47.06 
Geographic area, urban (%)  59.06  59.14  58.82 
Ethnicity (%)       
European  55.03  65.59  64.71 
Mapuche  44.97  34.41  35.29 
Level of education (%)       
Primary  20.13  10.87  23.53 
Secondary  40.94  53.26  64.71 
Technical/university  38.93  35.87  11.76 
BMI (kg/m2)  27.3 ± 3.6  28.0 ± 3.4  26.3 ± 4.1 
Nutritional status (%)       
Normal  26.85  19.35  47.06 
Overweight  49.66  50.54  29.41 
Obese  23.49  30.11  23.53 
Waist circumference (cm)  95.0 ± 10.5  97.6 ± 11.3  91.9 ± 11.0 
Body fat (%)  29.1 ± 4.8  29.7 ± 4.1  27.9 ± 5.2 

Data reported in terms of mean and standard deviation (SD) for continuous variables and percentage (%) for categorical variables.

BMI: body mass index; PA: physical activity.

Table 2.

Frequency of the MC4R genotype (rs483145).

rs483145  No.  Frequency (%)  Allele frequency  p value for HWE 
TT  149  57.5  0.754  0.616 
TA  93  35.9     
AA  17  6.6  0.245   

HWE: Hardy–Weinberg equilibrium.

The results for the association between the rs483145 SNP of the MC4R gene and markers of adiposity are presented in Table 3. No significant associations were identified between the risk genotype and the markers of adiposity studied (body weight, BMI, waist circumference, body fat percentage, hip circumference and waist-to-hip ratio). For body weight, values of 70.3 kg for the protective TT genotype, 71.1 kg for the TA genotype and 69.4 kg for the AA genotype were seen in the non-adjusted model (Model 0). For all other markers of adiposity, apart from waist-to-hip ratio, similar variations in increases were seen for the TA genotype and similar variations in decreases were seen for the AA genotype, in Model 0 and Model 1, but they were not statistically significant.

Table 3.

Association between the genotype of the MC4R gene (rs483145) and markers of obesity.

  TT  TA  AA  Effect of the additive genetic model  p value 
Body weight (kg)
Model 0  70.3 (68.6; 72.0)  71.1 (69.0; 73.3)  69.4 (64.4; 74.4)  0.17 (−1.89; 2.24)  0.868 
Model 1  70.0 (68.4; 71.6)  71.5 (69.4; 73.5)  69.8 (65.0; 74.5)  0.66 (−1.31; 2.63)  0.510 
Model 2  70.1 (68.2; 72.0)  70.2 (67.9; 72.6)  74.6 (68.8; 80.3)  0.10 (−2.92; 3.13)  0.944 
BMI (kg/m2)
Model 0  27.3 (26.7; 27.9)  28.0 (27.3; 28.8)  26.3 (24.5; 28.0)  0.10 (−0.62; 0.83)  0.772 
Model 1  27.4 (26.8; 27.9)  27.9 (27.1; 28.6)  26.6 (24.9; 28.3)  0.03 (−0.65; 0.72)  0.929 
Model 2  27.7 (27.1; 28.4)  27.5 (26.7; 28.4)  28.1 (26.0; 30.2)  −0.18 (−1.28; 0.92)  0.746 
Waist circumference (cm)
Model 0  95.0 (93.2; 96.8)  97.6 (95.4; 99.9)  91.9 (86.7; 97.1)  0.45 (−1.72; 2.63)  0.683 
Model 1  95.0 (93.3; 96.8)  97.6 (95.3; 99.8)  91.9 (86.7. 97.2)  0.41 (−1.77; 2.59)  0.711 
Model 2  95.0 (92.8; 97.2)  97.1 (94.2; 99.9)  96.9 (90.0; 103.7)  2.01 (−1.57; 5.61)  0.269 
Body fat percentage
Model 0  29.1 (28.4; 29.9)  29.7 (28.8; 30.7)  28.0 (25.8; 30.2)  0.01 (−0.91; 0.93)  0.988 
Model 1  29.2 (28.6; 29.9)  29.5 (28.6; 30.4)  28.3 (26.2; 30.4)  −0.14 (−1.00; 0.72)  0.540 
Model 2  29.0 (28.3; 29.9)  28.6 (27.5; 29.7)  30.0 (27.4; 32.6)  −0.43 (−1.81; 0.94)  0.534 
Hip circumference (cm)
Model 0  110.5 (108.9; 112.2)  113.3 (111.2; 115.4)  108.1 (103.2; 113.0)  0.68 (−1.36; 2.73)  0.512 
Model 1  110.6 (109.0; 112.3)  113.0 (110.9; 115.1)  108.4 (103.5; 113.2)  0.55 (−1.47; 2.58)  0.593 
Model 2  110.5 (108.3; 112.6)  112.1 (109.3; 114.8)  111.9 (105.3; 118.6)  1.60 (−1.87; 5.08)  0.363 
Waist-to-hip ratio
Model 0  0.95 (0.93; 0.96)  0.93 (0.91; 0.95)  0.92 (0.88; 0.96)  −0.01 (−0.03; 0.00)  0.106 
Model 1  0.94 (0.93; 0.96)  0.94 (0.92; 0.96)  0.91 (0.87; 0.95)  −0.01 (−0.02; 0.00)  0.179 
Model 2  0.93 (0.92; 0.95)  0.93 (0.91; 0.95)  0.92 (0.86; 0.97)  0.00 (−0.02; 0.02)  0.983 

Data reported in terms of mean and 95% CI by genotype. The additive genetic model indicates the average increase in the adiposity variable for each additional copy of the risk variant (A). This additive effect and its respective 95% CI were determined by means of linear regression. The analyses were adjusted as follows: Model 0: not adjusted; Model 1: adjusted by age, sex, ethnicity, level of education, income, socioeconomic status and area of residence (urban/rural); and Model 2: adjusted for Model 1, but also for physical activity.

95% CI: 95% confidence interval; BMI: body mass index.

Discussion

This study shows the absence of a significant association between the rs483145 SNP of the MC4R gene and markers of obesity in the adult Chilean population. The frequency of the risk allele (A) was estimated at 24.5%; this value was similar to that reported in European databases as 1000 genomes, but considerably lower than that of Pima Indians, who had a frequency of 80%.22 In addition, the rs17782313 SNP of the MC4R gene, which is more prevalent in the Caucasian population (30%), had a frequency of just 3% in Pima Indians, indicating that MC4R genetic variation depends heavily on ethnicity. In contrast with our results, the rs483145 risk allele in Pima Indians was associated with an increase by 0.58 kg/m2 in BMI per extra risk allele copy, although it was not associated with abnormalities in other markers of adiposity, such as body fat percentage or energy expenditure.22 The discrepancy in the association between the rs483145 SNP and BMI in Chileans versus Pima Indians may be linked to particular ethnic characteristics or to specific interactions with environmental factors that differ in these two populations, as demonstrated by longitudinal studies in which interactions between genetic make-up and environmental factors affected the degree of obesity present.28,29 However, the possibility that the lack of association was due to low statistical power cannot be ruled out, since the study conducted in Pima Indians included 11,268 participants compared to the 259 individuals included in this study.

Prior studies have examined the relationship between MC4R and obesity in the Chinese population, but by means of analysis of the rs17782313 SNP, a polymorphism located at the distal locus, 317 kb from the SNP studied herein.23 The rs17782313 SNP has a prevalence of the risk allele of nearly 30% in Chilean children and adults; it does not show a higher rate in individuals with obesity.30–32 Interestingly, these studies also revealed that, in both children and adults with obesity, the rs17782313 risk allele was correlated with abnormal eating behaviours, such as decreased levels of satiety and greater enjoyment of meals, but did not manage to determine any association with BMI.30 Taken together, studies of different genetic variants of MC4R conducted in the Chinese population have consistently found a high prevalence of risk variants close to 30% in various regions of the gene, with no association with obesity, possibly due to limited sample sizes (<500). In Europeans, on the other hand, the presence of the rs17782313 risk allele was accompanied by an increase by 0.2 kg/m2 in BMI, 0.66 kg in body weight and 14% in the likelihood of being obese.8 In the case of rs12970134, another distal-locus SNP, genome-wide association studies (GWAS) in Asian and European populations revealed an increase by 1 cm in waist circumference per risk allele copy.8

For SNPs associated with changes in the MC4R protein sequence, such as rs2229616 (V103I) and rs52820871 (I251 L), meta-analysis studies with more than 10,000 participants revealed that the minor alleles reduce the risk of obesity by 20% and 50% and have a frequency of 2%–7% in different populations, including American, Asian and European populations.3,8 For both SNPs, a decrease in BMI by close to 0.8 kg/m2 per risk allele copy has been reported. This effect is associated with a decreased response on the part of the MC4R gene to AGRP-mediated orexigenic signalling.19 Studies in Europeans also revealed abnormalities in food behaviour associated with non-synonymous variants and polymorphisms of the MC4R gene, such as a preference for fat consumption, lower sucrose intake and decreased response to satiety.33,34 The different studies reviewed revealed that the association between obesity and polymorphisms in MC4R and nearby loci are due to changes in the hypothalamic axis of appetite control, associated with response to POMC derivatives or AGRP, but also with regulation of macronutrient consumption in the amygdala, and with thermal homeostasis, associated with oxygen consumption in adipose tissue.9,34,35 Although the genetic variant analysed in this study is not located in the coding sequence, and therefore does not involve direct changes in MC4R protein function, its location towards the 5′ end of the gene renders it a potential cis-regulatory element with a potential impact on levels of MC4R expression. Should this be proven through, for example, expression quantitative trait loci (eQTL) studies, it might be postulated that carriers of the rs483145 SNP of MC4R could benefit from treatment with MC4R agonists which might increase signalling decreased by the effects of potentially lower receptor expression. Recently, the United States Food and Drug Administration (FDA) approved setmelanotide to treat severe obesity in patients with a POMC synthesis or leptin receptor deficiency, rendering it the first MC4R agonist to be approved for pharmacological use.

The limitations of this study included its limited sample size and the fact that it selected a population with an average age under 40 with no history of metabolic diseases. This study's limited sample size precluded analyses comparing men to women (112 and 147) or individuals of Mapuche descent to individuals of European descent (153 and 106). However, a lower prevalence of the risk allele could be seen in the Mapuche population (35% versus 65%). It should be noted that, in previously published studies in the same cohort, we identified positive associations between, on the one hand, genetic variants of FTO, TCF7 and SLC16A11 and, on the other hand, markers of obesity.36–38 The above suggests that any hidden association between the rs483145 SNP of the MC4R gene and obesity would have a significantly lower strength of association than those reported for FTO, TCF7 and SLC16A11. In addition, given that differences in food behaviours have been found for other SNPs of this gene, it would be useful to conduct similar studies for rs483145.16

Conclusions

Our results and the studies reported reveal the importance of ethnicity as a factor in the prevalence of the different SNPs of the MC4R gene, as well as the relationship thereof to markers of obesity. The different associations presented by other genetic variants of MC4R with obesity (predisposition versus protection) complexify the relationship between MC4R and obesity, revealing the need to study the prevalence of the various polymorphisms of MC4R in the different populations and their relationship to obesity, eating behaviours and metabolism. In particular, the rs483145 genetic variant was not found to be associated with obesity in the Chilean population, breaking with findings in Pima Indians.22 This study contributes new information that sheds light on the relationship between polymorphisms in the MC4R gene and obesity. This information could be taken into account in the design of primary prevention programmes and personalised treatment strategies in the Chilean population.

Conflicts of interest

The authors declare that they have no conflicts of interest.

Funding

This research has not received specific funding from public sector agencies, the commercial sector or non-profit organisations.

References
[1]
WHO. Obesity: preventing and managing the global epidemic. World Health Organization. Available from: http://www.who.int/nutrition/publications/obesity/WHO_TRS_894/en/. [Accessed 9 December 2019].
[2]
Collaboration NCDRF.
Trends in adult body-mass index in 200 countries from 1975 to 2014: a pooled analysis of 1698 population-based measurement studies with 19.2 million participants.
Lancet, 387 (2016), pp. 1377-1396
[3]
OCDE OplCyDE. Obesity Update 2017. Available from: https://www.oecd.org/els/health-systems/Obesity-Update-2017.pdf2017. [Accessed 9 December 2019].
[4]
MINSAL. Encuesta Nacional de Salud 2016-2017 Ministerio de Salud. Chile. 2017. Available from: http://www.minsal.cl/wp-content/uploads/2017/11/ENS-2016-17_PRIMEROS-RESULTADOS.pdf. [Accessed 9 December 2019].
[5]
F. Petermann, M.A. Martinez-Sanguinetti, M. Villagran, M. Ulloa, G. Nazar, C. Troncoso-Pantoja, et al.
From a global view to the Chilean context: which factors have influenced the development of obesity in Chile? (Chapter 1).
Rev Chil Nutr, 47 (2020), pp. 299-306
[6]
K. Rohde, M. Keller, L. la Cour Poulsen, M. Bluher, P. Kovacs, Y. Bottcher.
Genetics and epigenetics in obesity.
Metabolism, 92 (2019), pp. 37-50
[7]
S.S. Dong, Y.J. Zhang, Y.X. Chen, S. Yao, R.H. Hao, Y. Rong, et al.
Comprehensive review and annotation of susceptibility SNPs associated with obesity-related traits.
Obes Rev, 19 (2018), pp. 917-930
[8]
R.J. Loos, C.M. Lindgren, S. Li, E. Wheeler, J.H. Zhao, I. Prokopenko, et al.
Common variants near MC4R are associated with fat mass, weight and risk of obesity.
Nat Genet, 40 (2008), pp. 768-775
[9]
I. Gantz, T.M. Fong.
The melanocortin system.
Am J Physiol Endocrinol Metab, 284 (2003), pp. E468-474
[10]
Y.X. Tao.
The melanocortin-4 receptor: physiology, pharmacology, and pathophysiology.
Endocr Rev, 31 (2010), pp. 506-543
[11]
O. Ilnytska, G. Argyropoulos.
The role of the Agouti-Related Protein in energy balance regulation.
Cell Mol Life Sci, 65 (2008), pp. 2721-2731
[12]
P. Kühnen, H. Krude, H. Biebermann.
Melanocortin-4 receptor signalling: importance for weight regulation and obesity treatment.
Trends Mol Med, 25 (2019), pp. 136-148
[13]
S. Sharma, A.S. Garfield, B. Shah, B. Kleyn, I. Ichetovkin, I.H. Moeller, et al.
Current mechanistic and pharmacodynamic understanding of melanocortin-4 receptor activation.
[14]
K. Clément, E. van den Akker, J. Argente, A. Bahm, W.K. Chung, H. Connors, et al.
Efficacy and safety of setmelanotide, an MC4R agonist, in individuals with severe obesity due to LEPR or POMC deficiency: single-arm, open-label, multicentre, phase 3 trials.
Lancet Diabetes Endocrinol, 8 (2020), pp. 960-970
[15]
A. Markham.
Setmelanotide: first approval.
[16]
I.S. Farooqi, G.S. Yeo, J.M. Keogh, S. Aminian, S.A. Jebb, G. Butler, et al.
Dominant and recessive inheritance of morbid obesity associated with melanocortin 4 receptor deficiency.
J Clin Invest J, 106 (2000), pp. 271-279
[17]
D. Huszar, C.A. Lynch, V. Fairchild-Huntress, J.H. Dunmore, Q. Fang, L.R. Berkemeier, et al.
Targeted disruption of the melanocortin-4 receptor results in obesity in mice.
[18]
V. Turcot, Y. Lu, H.M. Highland, C. Schurmann, A.E. Justice, R.S. Fine, et al.
Protein-altering variants associated with body mass index implicate pathways that control energy intake and expenditure in obesity.
Nat Genet, 50 (2018), pp. 26-41
[19]
R.J. Loos.
The genetic epidemiology of melanocortin 4 receptor variants.
Eur J Pharmacol, 660 (2011), pp. 156-164
[20]
B. Xi, G.R. Chandak, Y. Shen, Q. Wang, D. Zhou.
Association between common polymorphism near the MC4R gene and obesity risk: a systematic review and meta-analysis.
[21]
D.P. Zobel, C.H. Andreasen, N. Grarup, H. Eiberg, T.I.A. Sorensen, A. Sandbæk, et al.
Variants near MC4R are associated with obesity and influence obesity-related quantitative traits in a population of middle-aged people: studies of 14,940 Danes.
Diabetes, 58 (2009), pp. 757-764
[22]
Y.L. Muller, M.S. Thearle, P. Piaggi, R. Hanson, D. Hoffman, B. Gene, et al.
Common genetic variation in and near the melanocortin 4 receptor gene (MC4R) is associated with body mass index in American Indian adults and children.
Hum Genet, 133 (2014), pp. 1431-1441
[23]
C.A. Celis-Morales, F. Perez-Bravo, L. Ibanes, R. Sanzana, E. Hormazabal, N. Ulloa, et al.
Insulin resistance in Chileans of European and indigenous descent: evidence for an ethnicity x environment interaction.
[24]
A. Scafoglieri, J. Tresignie, S. Provyn, M. Marfell-Jones, T. Reilly, I. Bautmans, et al.
Prediction of segmental lean mass using anthropometric variables in young adults.
J Sports Sci, 30 (2012), pp. 777-785
[25]
Y. Kimura, M. Kobayashi, M. Asari, I. Higuchi, K. Narumi, A. Furugen, et al.
Genetic variations in the monocarboxylate transporter genes (SLC16A1, SLC16A3, and SLC16A11) in the Japanese population.
Drug Metab Pharmacokinet, 33 (2018), pp. 215-218
[26]
E. Apud, P.R. Jones.
Validity of skinfold thickness as an estimation of fatty mass, with reference to the equations of Durnin and Womersley.
Rev Med Chil, 108 (1980), pp. 807-813
[27]
P.S. Freedson, E. Melanson, J. Sirard.
Calibration of the Computer Science and Applications, Inc. accelerometer.
Med Sci Sports Exerc, 30 (1998), pp. 777-781
[28]
M. Brandkvist, J.H. Bjorngaard, R.A. Odegard, B.O. Asvold, E.R. Sund, G.A. Vie.
Quantifying the impact of genes on body mass index during the obesity epidemic: longitudinal findings from the HUNT Study.
BMJ, 366 (2019), pp. l4067
[29]
M. Villagran, F. Petermann, M.A. Martínez-Sanguinetti, C. Celis-Morales.
The interaction of our genes with the obesogenic environment [Article in Spanish].
Rev Med Chile, 147 (2019), pp. 1491-1496
[30]
M. Valladares, P. Dominguez-Vasquez, A.M. Obregon, G. Weisstaub, R. Burrows, A. Maiz, et al.
Melanocortin-4 receptor gene variants in Chilean families: association with childhood obesity and eating behavior.
Nutr Neurosci, 13 (2010), pp. 71-78
[31]
J.A. Vega, G. Salazar, M.I. Hodgson, L.R. Cataldo, M. Valladares, A.M. Obregon, et al.
Melanocortin-4 receptor gene variation is associated with eating behavior in Chilean adults.
Ann Nutr Metab, 68 (2016), pp. 35-41
[32]
J. Ho-Urriola, I.P. Guzman-Guzman, S.V. Smalley, A. González, G. Weisstaub, P. Dominguez-Vasquez, et al.
Melanocortin-4 receptor polymorphism rs17782313: association with obesity and eating in the absence of hunger in Chilean children.
Nutrition, 30 (2014), pp. 145-149
[33]
L.A. Lotta, J. Mokrosinski, E. Mendes de Oliveira, C. Li, S.J. Sharp, J. Luan, et al.
Human gain-of-function MC4R variants show signaling bias and protect against obesity.
[34]
L. Morell-Azanza, A. Ojeda-Rodriguez, J. Giuranna, M.C. Azcona-SanJulian, J. Hebebrand, A. Marti, et al.
Melanocortin-4 receptor and lipocalin 2 gene variants in Spanish children with abdominal obesity: effects on BMI-SDS after a lifestyle intervention.
[35]
A.A. van der Klaauw, J.M. Keogh, E. Henning, C. Stephenson, S. Kelway, V.M. Trowse, et al.
Divergent effects of central melanocortin signalling on fat and sucrose preference in humans.
Nat Commun, 7 (2016), pp. 13055
[36]
F. Petermann, M. Villagrán, C. Troncoso, L. Mardones, A.M. Leiva, M.A. Martinez, et al.
Association between FTO (ns9939609) genotype and adiposity markers in Chilean adults [Article in Spanish].
Rev Med Chil, 146 (2018), pp. 717-726
[37]
F. Petermann-Rocha, N. Lasserre-Laso, M. Villagrán, L. Mardones, M.A. Martinez, A.M. Leiva, et al.
Association of the TCF7L2 (RS7903146) genotype with adiposity and metabolic markers in the Chilean adult population [Article in Spanish].
Rev Med Chil, 147 (2019), pp. 965-976
[38]
L. Mardones, F. Petermann-Rocha, M.A. Martinez-Sanguinetti, A.M. Leiva, C. Troncoso-Pantoja, M. Martorell, et al.
Genetic variants in the SLC16A11 gene are associated with increased BMI and insulin levels in nondiabetic Chilean population.
Arch Endocrinol Metab, (2021),

Please cite this article as: Mardones L, Parra-Valencia E, Petermann-Rocha F, Martínez-Sanguinetti MA, Leiva-Ordoñez AM, Lasserre-Laso N, et al. El polimorfismo rs483145 del gen MC4R no se asocia con obesidad en población chilena: resultados del estudio GENADIO. Endocrinol Diabetes Nutr. 2022;69:254–261.

Descargar PDF
Opciones de artículo
es en pt

¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

Are you a health professional able to prescribe or dispense drugs?

Você é um profissional de saúde habilitado a prescrever ou dispensar medicamentos