The authors regret that we have detected an error in the originally published version of the above article that led to a misinterpretation of the antibiotic susceptibility test of the Campylobacter fetus ssp. fetus (Cff) isolate. There, we indicated that the minimum inhibitory concentration to various antibiotics was assessed using the commercial kit “SensititreTM CAMPY AST Plate®”. However, this test was actually assessed using an alternative product from the same provider (“SensititreTM CAMPY2”), which has a different plate layout and reference chart. The assay was conducted with the alternative product, but the results were invertedly interpreted with the chart of the former product. Using the corresponding chart of the kit used to interpret the results, this Cff strain was resistant to nalidixic acid and sensitive to gentamicin, azithromycin, erythromycin, ciprofloxacin, clindamycin, florfenicol, and tetracycline (Table 1). We have repeated the assay three times and obtained the same results consistently. Thus, this is not a multidrug resistant strain as originally reported.
Minimal inhibitory concentration (MIC) breakpoints to determine antimicrobial susceptibility in the isolated Campylobacter fetus subsp. fetus strain.
Antimicrobial | MIC breakpoint (μg/ml) | MIC for the isolated Cff strain (μg/ml) | |
Resistant | Susceptible | ||
Gentamicin | ≥ 4 | ≤ 2 | 0.5 |
Azithromycin | ≥ 0.5 | ≤ 0.25 | 0.25 |
Erythromycin | ≥ 8 | ≤ 4 | 0.5 |
Ciprofloxacin | ≥ 1 | ≤ 0.5 | 0.25 |
Nalidixic acid | ≥ 32 | ≤ 16 | >64* |
Clindamycin | ≥ 1 | ≤ 0.5 | 0.5 |
Florfenicol | ≥ 8 | ≤ 4 | 0.5 |
Tetracycline | ≥ 2 | ≤ 1 | 0.5 |