Información del artículo
Fundamento
La paraoxonasa (PON1) es una éster hidrolasa relacionada con la eliminación de componentes oxidados de las lipoproteínas de baja densidad (LDL). El presente estudio analiza la relación entre el título de anticuerpos contra la LDL oxidada (Ac-LDLox), la actividad PON1 en suero y sus polimorfismos genéticos en 39 individuos normales y 39 pacientes supervivientes de un infarto de miocardio
Pacientes y métodosLos valores de Ac-LDLox se determinaron por enzimoinmunoanálisis. La actividad PON1 en suero se determinó mediante la hidrólisis de paraoxon. Los polimorfismos de la PON1 en las posiciones 55 y 192 se analizaron mediante amplificación por reacción en cadena de la polimerasa y análisis de restricción
ResultadosLos resultados obtenidos no demostraron ninguna relación significativa entre los valores de Ac-LDLox ni con la actividad PON1 en suero ni con sus polimorfismos, en sujetos controles o en pacientes con infarto de miocardio
Palabras clave:
Infarto de miocardio
LDL oxidada
Paraoxonasa
Polimorfismos genéticos
Background
Paraoxonase (PON1) is an ester hydrolase related to the elimination of oxidized compounds of LDL particles. The aim of the present study was to analyze the relationship between the antibody titre against oxidized LDL (Ac-LDLox), PON1 activity in serum and its genetic polymorphisms in 39 normal subjects and 39 survivor patients of a myocardial infarction
Patients and methodsAc-LDLox levels were determined by enzymoimmunoassay. PON1 activity was measured by the hydrolysis of paraoxon. PON1 polymorphisms at positions 55 and 192 were analyzed by polymerase chain reaction and restriction isotyping
ResultsResults obtained did not show any significant relationship between Ac-LDLox levels neither with PON1 activity in serum nor with its polymorphisms, in control subjects or in patients with myocardial infarction
Key words:
Myocardial infarction
Oxidized LDL
Paraoxonase
Genetic polymorphisms
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